AFLP‐based PCR markers that differentiate spot and net forms of <i>Pyrenophora teres</i>

Leišová, L.; Kučera, L.; Minarikova, V.

doi:10.1111/j.1365-3059.2005.01117.x

Cited by 43 publications

(35 citation statements)

References 23 publications

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“…The markers used proved to be highly polymorphic and revealed clear differences in the distribution of alleles for 91 % of the loci tested between the two fungal types. This provides further evidence that the two formae specialis of P. teres are indeed genetically distinct from one another as has been shown in previous studies (Williams et al 2001;Leisova et al 2005a;Bakonyi & Justesen 2007;Keiper et al 2008). Because the forms of net blotch can be difficult to differentiate based on disease symptoms or spore morphology, the 14 STM markers that amplified distinct PTT-or PTM-specific allelic fragments can thus potentially be used as diagnostic markers for accurate disease diagnosis.…”

Section: Discussionmentioning

confidence: 61%

Genetic structure of South Australian Pyrenophora teres populations as revealed by microsatellite analyses

2010

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Section: Discussionmentioning

confidence: 61%

Genetic structure of South Australian Pyrenophora teres populations as revealed by microsatellite analyses

2010

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“…The amplification with four designed PCR primer pairs provided P. teres form-specific products. No cross-reaction was observed with DNA of several other species, such as P. tritici-repentis and P. graminea (Leisova et al 2005). Pyrenophora graminea is the causal agent of barley leaf stripe disease (Mokrani et al 2012).…”

Section: Molecular Phylogenymentioning

confidence: 91%

“…The data suggested that the RAPD technique can be used to tag genetic determinants for virulence in P. teres f. teres (Weiland et al 1999). Specific polymerase chain reaction (PCR) primers were developed from amplified fragment length polymorphism (AFLP) fragments of P. teres, in order to distinguish the two forms, P. teres f. teres (which cause net form blotch on barley leaves) and P. teres f. maculata (which causes spot form); the two forms are morphologically very similar in culture (Leisova et al 2005). The PCR assay was certified with 60 samples of Pyrenophora species.…”

Section: Molecular Phylogenymentioning

confidence: 99%

One stop shop: backbones trees for important phytopathogenic genera: I (2014)

et al. 2014

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Many fungi are pathogenic on plants and cause significant damage in agriculture and forestry. They are also part of the natural ecosystem and may play a role in regulating plant numbers/density. Morphological identification and analysis of plant pathogenic fungi, while important, is often hampered by the scarcity of discriminatory taxonomic characters and the endophytic or inconspicuous nature of these fungi. Molecular (DNA sequence) data for plant pathogenic fungi have emerged as key information for diagnostic and classification studies, although hampered in part by non-standard laboratory practices and analytical methods. To facilitate current and future research, this study provides phylogenetic synopses for 25 groups of plant pathogenic fungi in the Ascomycota, Basidiomycota, Mucormycotina (Fungi), and Oomycota, using recent molecular data, up-to-date names, and the latest taxonomic insights. Lineagespecific laboratory protocols together with advice on their application, as well as general observations, are also provided. We hope to maintain updated backbone trees of these fungal lineages over time and to publish them jointly as new data emerge. Researchers of plant pathogenic fungi not covered by the present study are invited to join this future effort. Bipolaris, Botryosphaeriaceae,

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“…Wu et al (2003) using RFLP profiles were unable to separate the two forms and reported that the genetic distance between isolates of the two forms was similar to that of isolates among the same form. However, a recent report by Leisova et al (2005) based on AFLP data suggests that the two forms are distinct species.…”

Section: Introductionmentioning

confidence: 87%

Pathogenic variation in populations of Drechslera teres f. teres and D. teres f. maculata and differences in host cultivar responses

et al. 2006

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The current study examined the variability in the pathogenicity of populations of Drechslera teres f. teres and D. teres f. maculata (the net and spot forms of D. teres) from Ireland and northern Europe. A population of progeny isolates from a mating of net and spot forms was also examined. Significant variation in virulence was found both between and among net form and spot form isolates (p<0.001). In the Irish population, significant differences were found between the net and spot forms, with the spot form isolates more virulent (p<0.05). Progeny isolates were significantly more virulent than net form or spot form populations (p<0.001). Significant differences were found in cultivar reactions, with cv. Botnia most susceptible to both forms of the pathogen (p<0.001). Cultivar Boreal 94145, although quantitatively resistant, was found to be very susceptible to both forms of the pathogen and to progeny isolates. Cultivars CI 5791, CI 2330 and CI 9819 were all less susceptible to infection by both forms, but were more susceptible to spot form isolates. Significant correlations were found between whole plants and detached leaf experiments for the net form isolates only (p<0.001). This study illustrates the importance of including both net form and spot form isolates in resistance studies and the need for a clearer understanding for the genetic basis of resistance to the net and spot forms. It also highlights the limitations of using a detached leaf assay for screening of net blotch of barley.

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AFLP‐based PCR markers that differentiate spot and net forms of Pyrenophora teres

Cited by 43 publications

References 23 publications

Genetic structure of South Australian Pyrenophora teres populations as revealed by microsatellite analyses

Genetic structure of South Australian Pyrenophora teres populations as revealed by microsatellite analyses

One stop shop: backbones trees for important phytopathogenic genera: I (2014)

Pathogenic variation in populations of Drechslera teres f. teres and D. teres f. maculata and differences in host cultivar responses

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