AFLP Fingerprinting Analysis of Citrus Cultivars and Wild Accessions from Oman Suggests the Presence of Six Distinct Cultivars

Abstract

A study was conducted to evaluate genetic relatedness of 27 citrus cultivars and 6 wild citrus accessions using AFLP fingerprinting. The 27 citrus cultivars belonged to Citrus sinensis, C. aurantifolia, C. aurantium, C. paradise, C. reticulata, C. limon, C. latifolia, C. maxima, C. limettoides, C. limetta, C. medica and C. Jambhiri. The wild cultivars were obtained from Oman while the other cultivars originated from Oman and other countries. AFLP analysis using 4 primer pair combinations resolved 910 polymorphic alleles. All citrus cultivars and accessions had low genetic diversity (H = 0.0281 to 0.1300), with the percent polymorphic loci ranging from 8 to 35%. Populations of the six wild citrus accessions showed a very low level of genetic diversity (< 0.0700). Cluster analysis of the 33 cultivars and accessions showed that they share a high level of genetic similarity (81‒99%; mean = 92%). The six wild accessions clustered into two main clusters, with the analysis indicating that the six wild accessions may make up six distinct cultivars. The study provides information on the phylogeny of citrus cultivars and citrus diversity in Oman, a country through which citrus moved in the past from Asia to different African and European countries. In addition, it shows that some distinct citrus cultivars are present in this part of the world.

Sequences analysis of chloroplast psbA-trnH region in Citrus L. (Rutaceae) species from the Aegean region of Turkey

“…There are many studies in Citrus using various molecular markers, e.g. RAPD (Coletta Filho et al 1998;Nicolosi et al 2000), ISSR (Fang and Roose 1997), AFLP (Al-Nadabi et al 2018), SSR (Jannati et al 2009), as well as chloroplast DNA (Nicolosi et al 2000;Penjor et al 2010;Wali et al 2013;Oueslati et al 2016;Uchoi et al 2016;Sevindik and Yalçın 2018) and nuclear ribosomal DNA (nrDNA) markers (Amar et al 2014;Sun et al 2015). In this study, we obtained psbA-trnH sequences ranging from 426 to 470 nucleotides for 11 specimens.…”