AFLP genetic diversity analysis in Russian wheat aphid resistant wheat accessions

Srinivas, Girish; Huang, Yinghua; Carver, Brett F.; Mornhinweg, D. W.

doi:10.1007/s10681-011-0605-9

Cited by 4 publications

(3 citation statements)

References 34 publications

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“…The incorporation of resistance to insect pests in genetic materials is a methodology recommended by the ease of use and cost however, one must have prior knowledge of the main pests that affect the crop. Srinivas et al [11] describe that genetic improvement of crops for tolerance to biotic and abiotic factors is a major focus of breeding programs worldwide, because it is considered that the incorporation of insect resistance is considered the most effective and environmentally safe control method.…”

Section: Resultsmentioning

confidence: 99%

Ecological Survey of Pests and Natural Enemies in the Sour Passion Fruit Progenies

Rosado

Bruckner

Reis

et al. 2019

JEAI

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Aims: This experiment was to identify the major pests, natural enemies and verify the regarding the incidence of insect pests of progenies of Passiflora edulis to pests. Study Design: Experimental design was completely randomized blocks with twenty-three progenies and three replicates with four plants per plot. Place and Duration of Study: Experimental evaluations of the Federal University of Viçosa/Rio Paranaíba University Campus, Rio Paranaíba county, Minas Gerais, Brazil from May to September 2011. Methodology: The population fluctuation of insects, for characterization of their occurrence and identification of progenies with respect to their degree of resistance. Results: Among the monitored pests stood out, A. vanillae vanillae, D. juno juno e Dasiops sp. The correlation between the amount of Dione juno juno and the attacked leaf had a higher magnitude of occurrence in relation Agraulis vanillae vanillae. Conclusion: It was found that there were no differences between the sour passion fruit progenies and resistance to pests, and to verify that ants are important predators of pests of sour passion fruit.

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Section: Resultsmentioning

confidence: 99%

Ecological Survey of Pests and Natural Enemies in the Sour Passion Fruit Progenies

Rosado

Bruckner

Reis

et al. 2019

JEAI

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“…To raise the density of microsatellite markers available on a wheat genetic map, Somers et al (2004) constructed a high density microsatellite consensus map by mapping common microsatellites on each chromosome in 4 different mapping populations. Microsatellites can be exchanged between laboratories, and are highly transferable between populations (Song et al 2005, Sun et al 1998, 2003.…”

Section: İntroductionmentioning

confidence: 99%

Evaluation of Genetic Diversity Using Ssr Markers and Link With Drought Response of Azerbaijani Durum Wheat (Triticum Durum L.) Genotypes

Mammadova,

AMRI,

AKPAROV

et al. 2023

Preprint

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Genetic diversity of 46 azerbaijani durum wheat (triticum durum l.) Genotypes were screened using simple sequence repeats (ssrs). These accessions were collected from various bioclimatic regions of azerbaijan. Out of the used twenty-two primers, 13 primers showed polymorphism and were selected for the analyses. Among the genotypes under study, 31 alleles were detected. Highest number of alleles was detected in locus gwm 335 (on chromosome 5b) and on locus gwm 445 (on chromosome 2a) with 5 and 4 alleles, respectively. The lowest number of alleles was determined in locus gwm 617 with only 1 allele. For a, b and d genomes, the total number of alleles detected were 14, 15 and 2, respectively. Pic value between studied ssr markers was 0.912 and this result shows high genetic diversity between azerbaijani durum wheat genotypes. Therefore, these primers can be recommended for studying genetic diversity of azerbaijani durum wheat accessions. The genetic structure of the genotypes was analyzed and the upgma dendrogram revealed five major clusters using nei genetic distance index. The results revealed that ssr markers can efficiently evaluate genetic variation in the wheat samples. Based on the previous characterization of drought response of these genotypes, links could be established between the ssr markers and drought tolerance. If some of the ssr markers are confirmed for their association s with drought tolerance, then, they can be used as markers for identification of drought tolerant varieties needed to enhance wheat productivity for farmers dealing with harsh conditions.

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“…DNA-based molecular markers have provided a powerful method for genetic diversity assessment (Badr, El-Shazly & Mekki, 2012; Srinivas et al, 2012) and cultivar identification (Anand, Prabhu & Singh, 2012; Karaagac et al, 2014). Although genetic diversity of ryegrass accessions have been evaluated by amplified fragment length polymorphism (AFLP) (Guthridge et al, 2001), restricted fragment length polymorphisms (RFLP) (Sato et al, 1995), sequence-related amplified polymorphism (SRAP) (Huang et al, 2014a; Huang et al, 2014b) and randomly amplified polymorphic DNA (RAPD) (Bolaric et al, 2005; Vieira et al, 2004), simple sequence repeat (SSR) markers are still considered as a powerful tool for characterizing the genetic variability of cultivars.…”

Section: Introductionmentioning

confidence: 99%

Genetic variability evaluation and cultivar identification of tetraploid annual ryegrass using SSR markers

Nie

Huang

et al. 2019

PeerJ

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Annual ryegrass (Lolium multiflorum) is a widely used cool-season turf and forage grass with high productivity and ornamental characteristics. However, the abundant intra-cultivar genetic variability usually hampers the application of conventional techniques for cultivar identification. The objectives of this study were to: (1) describe an efficient strategy for identification of six tetraploid annual ryegrass cultivars and (2) investigate the genetic diversity based on SSR markers. A total of 242 reliable bands were obtained from 29 SSR primer pairs with an average of 8.3 bands for each primer pair and the average value of polymorphic information content (PIC) was 0.304. The result of analysis of molecular variance (AMOVA) revealed that 81.99% of the genetic variation occurred in within-cultivars and 18.01% among-cultivars. The principal coordinate analysis (PCoA) showed that the first two principal axes explain 8.57% (PC1) and 6.05% (PC2) of total variation, respectively. By using multi-bulk strategy based on different filtering thresholds, the results suggested that bands frequency of 40% could be used as a reliable standard for cultivar identification in annual ryegrass. Under this threshold, 12 SSR primer pairs (00-04A, 02-06G, 02-08C, 03-05A, 04-05B, 10-09E, 12-01A, 13-02H, 13-12D, 14-06F, 15-01C and 17-10D) were detected for direct identification of six tetraploid annual ryegrass cultivars, which could be incorporated into conservation schemes to protect the intellectual property of breeders, ensure purity for consumers, as well as guarantee effective use of cultivars in future.

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AFLP genetic diversity analysis in Russian wheat aphid resistant wheat accessions

Cited by 4 publications

References 34 publications

Ecological Survey of Pests and Natural Enemies in the Sour Passion Fruit Progenies

Ecological Survey of Pests and Natural Enemies in the Sour Passion Fruit Progenies

Evaluation of Genetic Diversity Using Ssr Markers and Link With Drought Response of Azerbaijani Durum Wheat (Triticum Durum L.) Genotypes

Genetic variability evaluation and cultivar identification of tetraploid annual ryegrass using SSR markers

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