AflSte20 Regulates Morphogenesis, Stress Response, and Aflatoxin Biosynthesis of Aspergillus flavus

Li, Ding; Qin, Ling; Wang, Yinchun; Xie, Qingchen; Li, Na; Wang, Shihua; Yuan, Jun

doi:10.3390/toxins11120730

Cited by 14 publications

(10 citation statements)

References 46 publications

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“…Both iturin A and fengycin reduced the relative abundance of Aspergillus and Gibberella. Aspergillus not only causes huge losses to agricultural production, but also has a harmful effect on the products via metabolic substance such as aflatoxins, which are the most toxic mycotoxins and are extremely harmful to human health (Li et al, 2019;Parish et al, 2019). Gibberella is a parasitic and pathogenic fungus of certain plants, and causes a variety of plant diseases, such as rice and corn ear rot (Parker et al, 2017).…”

Section: Effects Of Fengycin and Iturin A On Soil Microbial Community Structurementioning

confidence: 99%

Activity of Fengycin and Iturin A Isolated From Bacillus subtilis Z-14 on Gaeumannomyces graminis Var. tritici and Soil Microbial Diversity

et al. 2021

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Bacillus subtilis Z-14 can inhibit phytopathogenic fungi, and is used as a biocontrol agent for wheat take-all disease. The present study used the soil-borne fungus Gaeumannomyces graminis var. tritici (Ggt), which causes wheat take-all disease, and the soil microbial community as indicators, and investigated the antifungal effects of fengycin and iturin A purified from strain Z-14 using high performance liquid chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, respectively. The results showed that fengycin destroyed the internal structure of Ggt cells by digesting the cytoplasm and organelles, forming vacuoles, and inducing hyphal shrinkage and distortion. Iturin A induced cell wall disappearance, membrane degeneration, intracellular material shrinkage, and hyphal fragmentation. A biocontrol test demonstrated a 100% control effect on wheat take-all when wheat seedlings were treated with fengycin at 100 μg/ml or iturin A at 500 μg/ml. Iturin A and fengycin both reduced the relative abundance of Aspergillus and Gibberella. At the genus level, iturin A reduced the relative abundance of Mortierella and Myrothecium, while fengycin reduced that of Fusarium. Only fengycin treatment for 7 days had a significant effect on soil bacterial diversity.

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Section: Effects Of Fengycin and Iturin A On Soil Microbial Community Structurementioning

confidence: 99%

Activity of Fengycin and Iturin A Isolated From Bacillus subtilis Z-14 on Gaeumannomyces graminis Var. tritici and Soil Microbial Diversity

et al. 2021

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“…In S. cerevisiae , Msb2 regulated the activity of p21‐activated protein kinases (PAKs) Ste20 and Cla4 (Tanaka et al ., 2014). Similarly, the report from previous study further verified that the loss of PAKs Aflste20 would lead to the impaired colonization and reduced AFB 1 production (Li et al ., 2019). It hints that genes in HOG pathway are important for AFB 1 production in A. flavus .…”

Section: Discussionmentioning

confidence: 99%

The membrane mucin Msb2 regulates aflatoxin biosynthesis and pathogenicity in fungus Aspergillus flavus

Qin

Zhao

et al. 2020

Microbial Biotechnology

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As a pathogenic fungus, Aspergillus flavus can produce carcinogenic aflatoxins (AFs), which poses a great threat to crops and animals. Msb2, the signalling mucin protein, is a part of mitogen-activated protein kinase (MAPK) pathway which contributes to a range of physiological processes. In this study, the roles of membrane mucin Msb2 were explored in A. flavus by the application of gene disruption. The deletion of msb2 gene (Dmsb2) caused defects in vegetative growth, sporulation and sclerotia formation when compared to WT and complement strain (Dmsb2 C) in A. flavus. Using thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) analysis, it was found that deletion of msb2 down-regulated aflatoxin B 1 (AFB 1) synthesis and decreased the infection capacity of A. flavus. Consistently, Msb2 responds to cell wall stress and osmotic stress by positively regulating the phosphorylation of MAP kinase. Notably, Dmsb2 mutant exhibited cell wall defect, and it was more sensitive to inhibitor caspofungin when compared to WT and Dmsb2 C. Taking together, these results revealed that Msb2 plays key roles in morphological development process, stresses adaptation, secondary metabolism and pathogenicity in fungus A. flavus.

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“…According to the dry weight of the mycelium, the samples were loaded on a TLC plate in the same proportion as the dry weight ( 59 ). The HPLC method referred to the aforementioned method ( 71 ), and aflatoxin was detected by an HPLC system (Water) on a MYCOTOX reversed phase C18 column.…”

Section: Methodsmentioning

confidence: 99%

The regulatory role of the Aspergillus flavus core retromer complex in aflatoxin metabolism

Wang¹,

Wang²,

Liu³

et al. 2022

Journal of Biological Chemistry

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AflSte20 Regulates Morphogenesis, Stress Response, and Aflatoxin Biosynthesis of Aspergillus flavus

Cited by 14 publications

References 46 publications

Activity of Fengycin and Iturin A Isolated From Bacillus subtilis Z-14 on Gaeumannomyces graminis Var. tritici and Soil Microbial Diversity

Activity of Fengycin and Iturin A Isolated From Bacillus subtilis Z-14 on Gaeumannomyces graminis Var. tritici and Soil Microbial Diversity

The membrane mucin Msb2 regulates aflatoxin biosynthesis and pathogenicity in fungus Aspergillus flavus

The regulatory role of the Aspergillus flavus core retromer complex in aflatoxin metabolism

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