After the Prestige oil spill modifications in NO production and other parameters related to the immune response were detected in hemocytes of Mytilus galloprovincialis

Novas, A. J.; Barcia, Ramiro; Ramos-Martı́nez, Juan Ignacio

doi:10.1016/j.aquatox.2007.09.011

Cited by 16 publications

(8 citation statements)

References 38 publications

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“…Oxidative activity was evaluated through the production of reactive oxygen species and expressed in Xow cytometric arbitrary units (A.U. Novas et al (2007) reported a longterm alteration of hemocyte-related immune parameters in the mussel Mytilus galloprovincialis, including a decrease in nitric oxide production and an increased sensitivity of hemocytes to apoptosis and necrosis. In contrast, Ordás et al (2007) did not observe an eVect of the fuel oil spilled by the Prestige on hemocyte immune parameters when M. galloprovincialis was maintained in an aquarium and exposed to the fuel oil for 4 months.…”

Section: Discussionmentioning

confidence: 99%

Hemocyte parameters of the Pacific oyster Crassostrea gigas a year after the Hebei Spirit oil spill off the west coast of Korea

et al. 2010

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In marine bivalves, hemocytes support various physiological functions, including immune defense, nutrient transport, shell repair, and homeostatic maintenance. Although the eVects of marine contaminants on the immunological functions of bivalves have been extensively investigated, the impacts of oil spills are not well understood. Therefore, we investigated hemocyte parameters in the PaciWc oyster Crassostrea gigas 13 months after the Hebei Spirit oil spill (December 2007) oV the west coast of Korea. The parameters studied included hemocyte concentration and mortality, relative proportion of hemocyte populations, and immunological functions such as phagocytosis and oxidative activity using Xow cytometry. These immune-related parameters in oysters damaged by the oil spill were also compared to control oysters that were collected from an area unaVected by the spill. The Xow cytometry study indicated that granulocyte population, phagocytic capacity, and reactive oxygen species production in oysters exposed to crude oil 13 months prior were depressed compared to the unexposed control oysters. Our data suggest that immunocompetence in oysters aVected by the oil spill had not fully recovered 1 year after the accident, although more detailed studies on the physiology and disease resistance should be performed.

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Section: Discussionmentioning

confidence: 99%

Hemocyte parameters of the Pacific oyster Crassostrea gigas a year after the Hebei Spirit oil spill off the west coast of Korea

et al. 2010

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“…Bivalves and samples were constantly kept on ice. The syringe was prefilled with sterile Alseve medium (20.8 g L −1 glucose; 8 g L −1 sodium citrate; 3.36 g L −1 EDTA; 22.5 g L −1 NaCl; pH 7; 920 mOsm; Novas et al [42]), used as anticoagulant and nutritive medium for the cells (1:5, medium:hemolymph), except for phagocytic activity assays.…”

Section: Hemocyte Analysesmentioning

confidence: 99%

Separate and combined effects of neurotoxic and lytic compounds of Alexandrium strains on Mytilus edulis feeding activity and hemocyte function

Bianchi

Langeloh

Tillmann

et al. 2019

Fish & Shellfish Immunology

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Multiple toxic and bioactive compounds produced by Alexandrium spp. cause adverse effects on bivalves, but these effects are frequently difficult to attribute to a single compound class. To disentangle the effect of neurotoxic vs lytic secondary metabolites, we exposed blue mussels to either a paralytic shellfish toxin (PST) producing Alexandrium spp. strain, or to an exclusively lytic compound (LC) producing strain, or a strain containing both compound classes, to evaluate the time dependent effects after 3 and 7 days of feeding. Tested parameters comprised signs of paralysis, feeding activity, and immune cell integrity (hemocyte numbers and viability; lysosomal membrane destabilization) and function (ROS production). Both compound classes caused paralysis and immune impairment. The only effect attributable exclusively to PST was increased phagocytic activity after 3 days and impaired feeding activity after 7 days, which curtailed toxin accumulation in digestive glands. Lysosomal membrane destabilization were more closely, but not exclusively, matched with LC exposure. Effects on circulating hemocyte integrity and immune related functions were mostly transient or remained stable within 7 days; except for increased lysosomal labialization and decreased extracellular ROS production when mussels were exposed to the toxin combination. M. edulis displays adaptive fitness traits to survive and maintain immune capacity upon prolonged exposure to environmentally relevant concentrations of PST and/or LC producing Alexandrium strains.

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“…해산 이매패류의 건강도를 평가하는 방법으로는 비만도 (condition index) 조사 (Pampanin et al 2005;Uddin et al 2007;Park et al 2008;Chávez-Villalba et al 2010), 조직 병리학적(histopathology) 관찰 (Ngo et al 2003(Ngo et al , 2004Gorinstein et al 2008;Park et al 2008;Yang et al 2010), 소화맹낭위축도(digestive gland atrophy) 조사 (Winstead et al 1995;Kang et al 2010), 체조성분 (biochemical composition) 분석 (Dridi et al 2007;Kang et al 2007Kang et al , 2011Li et al 2010), 혈구 면역력 측정 Park et al 2006;Novas et al 2007;da Silva et al 2008;Flye-Sainte-Marie et al 2009;Hégaret et al 2009, Li et al 2010) 등의 다양한 방법이 이용되고 있다. 해산 이매패류는 체액성 인자(humoral effectors)들과 혈구(hemocyte)로 구성된 선천 면역 시스템(innate immune system)을 보유하고 있으며, 혈구는 모든 조직세포 사이를 자유롭게 순환하며 내부 면역 외에도 영양분의 소화와 이 동, 패각과 조직의 수선, 분비 등에 관여를 하며 생리적 안 정상태를 유지하기 위해 다양한 변화를 나타낸다 (Cheng 2000;Chu 2000).…”

Section: 서 론unclassified

“…또한 중금속 외에도 해상에 원유 유출 사고로 인해 해산 이매패류의 혈구 기능들이 저하된 연구들도 있 다. 1996년 영국 Wales 해안에서 발생한 Sea Empress 유 류 유출로 인해 진주담치(Mytilus edulis)는 혈구의 식세포 능력과 과산화 이온(superoxide anion) 생산력이 줄어들었 으며 (Dyrynda et al 2000), 2002년 스페인에서 발생한 Prestige 유류 유출 사고로 지중해담치(M. galloprovincialis) 의 혈구 내 일산화질소(nitric oxide) 생산이 감소되었다 (Novas et al 2007…”

Section: 서 론unclassified

Assessment of Immune Parameters of the Wild Pacific Oyster (Crassostrea gigas) using a Flow Cytometry and Neutral Red Retention Assay

Hong¹,

Kang²,

Kim³

et al. 2012

Ocean and Polar Research

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Hemocyte parameters of the wild Pacific oyster Crassostrea gigas inhabiting intertidal zones in small bays (Gwangyang and Jinhae Bay) on the southern coast of Korea were evaluated using flow cytometry and neutral red retention (NRR) assay. Morphological features, cell count, mortality, DNA damage, phagocytosis, and lysosomal membrane stability of hemocytes were analyzed. Three types of hemocytes were identified in the oyster hemolymph: granulocytes, hyalinocytes, and blast-like cells. Immune related functions of hemocyte including phagocytosis and lysosomal membrane stability were significantly different among the study areas (P < 0.05), while cell count, mortality, and DNA damage of hemocytes were not significantly different. In Gwangyang Bay, phagocytosis of granulocytes and lysosomal membrane stability of oyster hemocytes inhabiting inside bay were significantly lower than those of oyster hemocytes in outside bay (P < 0.05), indicating that oysters in inside bay of Gwangyang were relatively suppressed the immunological function in hemocytes. Contrary to Gwangyang Bay, immune parameters of oyster hemocytes in Jinhae Bay not showed the difference between sampling sites. In conclusion, flow cytometry and NRR assay using oyster hemocyte has a powerful tool to investigate the cell level in a short time due to no-preprocessing of material.

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After the Prestige oil spill modifications in NO production and other parameters related to the immune response were detected in hemocytes of Mytilus galloprovincialis

Cited by 16 publications

References 38 publications

Hemocyte parameters of the Pacific oyster Crassostrea gigas a year after the Hebei Spirit oil spill off the west coast of Korea

Hemocyte parameters of the Pacific oyster Crassostrea gigas a year after the Hebei Spirit oil spill off the west coast of Korea

Separate and combined effects of neurotoxic and lytic compounds of Alexandrium strains on Mytilus edulis feeding activity and hemocyte function

Assessment of Immune Parameters of the Wild Pacific Oyster (Crassostrea gigas) using a Flow Cytometry and Neutral Red Retention Assay

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