Ag and peptide co-decorate polyetheretherketone to enhance antibacterial property and osteogenic differentiation

Yu, Yue; Sun, Yimin; Zhou, Xiong; Mao, Yurong; Liu, Yunxiu; Ye, Ling; Kuang, Li; Yang, Jing; Deng, Yi

doi:10.1016/j.colsurfb.2020.111492

Cited by 35 publications

(18 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other AMPs than GL13K were coated on PEEK substrates [ 17 , 30 , 31 , 32 , 33 ]. The conventional peptide coating procedure for a PEEK surface involves roughing using sandpaper, plasma spraying, and alkaline and sulfonating or acid treatments to activate the PEEK surface [ 7 , 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

Facilitating GL13K Peptide Grafting on Polyetheretherketone via 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide: Surface Properties and Antibacterial Activity

Kumar

Tuong

et al. 2021

IJMS

View full text Add to dashboard Cite

In the present work, the antimicrobial peptide (AMP) of GL13K was successfully coated onto a polyetheretherketone (PEEK) substrate to investigate its antibacterial activities against Staphylococcus aureus (S. aureus) bacteria. To improve the coating efficiency, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) was mixed with a GL13K solution and coated on the PEEK surface for comparison. Both energy-dispersive X-ray spectroscopy (EDX) and X-ray photoelectron spectroscopy (XPS) data confirmed 30% greater peptide coating on PEEK/GL13K-EDC than PEEK without EDC treatment. The GL13K graft levels are depicted in the micrograms per square centimeter range. The PEEK/GL13K-EDC sample showed a smoother and lower roughness (Rq of 0.530 µm) than the PEEK/GL13K (0.634 µm) and PEEK (0.697 µm) samples. The surface of the PEEK/GL13K-EDC was more hydrophilic (with a water contact angle of 24°) than the PEEK/GL13K (40°) and pure PEEK (89°) samples. The pure PEEK disc did not exhibit any inhibition zone against S. aureus. After peptide coating, the samples demonstrated significant zones of inhibition: 28 mm and 25 mm for the PEEK/GL13K-EDC and PEEK/GL13K samples, respectively. The bacteria-challenged PEEK sample showed numerous bacteria clusters, whereas PEEK/GL13K contained a little bacteria and PEEK/GL13K-EDC had no bacterial attachment. The results confirm that the GL13K peptide coating was able to induce antibacterial and biofilm-inhibitory effects. To the best of our knowledge, this is the first report of successful GL13K peptide grafting on a PEEK substrate via EDC coupling. The present work illustrates a facile and promising coating technique for a polymeric surface to provide bactericidal activity and biofilm resistance to medical implantable devices.

show abstract

Section: Introductionmentioning

confidence: 99%

Facilitating GL13K Peptide Grafting on Polyetheretherketone via 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide: Surface Properties and Antibacterial Activity

Kumar

Tuong

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…The results are consistent with the above analysis, that both PDA-coating and the sustained release of pBMP-2 from the microspheres on PEEK would promote cell differentiation. [62][63][64] The NPEEK-PDA-(PLGA-NH 2 @pBMP-2) scaffold not only possessed favorable biocompatibility, but also obtained a positive osteogenic induction effect on rBMSCs, making it a potential restoration material for bone defects.…”

Section: Discussionmentioning

confidence: 99%

Construction of a BMP‐2 gene delivery system for polyetheretherketone bone implant material and its effect on bone formation in vitro

Qin

Gan

et al. 2022

J Biomed Mater Res

View full text Add to dashboard Cite

Polyetheretherketone (PEEK) has been widely investigated for improving its biological inert to enable it to achieve stronger osteogenic capability and to be a promising material in implant fields. The most important mechanism that makes a successful implantation is osteointegration. Surface modification is an appropriate method to maintain the excellent mechanical properties of PEEK and simultaneously endow PEEK certain biological characters. In this work, we attempted to shape the nanotopography of PEEK surface by nitrogen low-temperature plasma and polydopamine coating on the surface as a secondary reaction platform to bond the aminated poly (lactic-co-glycolic acid) (PLGA) microspheres encapsulating the BMP-2 gene for enhancing the biological activity. Scanning electron microscope, atomic force microscopy, X-ray photoelectron spectroscopy and water contact angle (CA) measurements were applied to characterize the surface of modified or untreated PEEK. Surface characterization showed that the modification was successfully performed on PEEK including a rougher and more hydrophilic surface with nanotopographic features. The influence on cell adhesion, proliferation and differentiation was evaluated by culturing of rat bone marrow mesenchymal stem cells on different modified PEEK substrates in vitro. The biological results indicated that the low-temperature plasma treatment and PDA-coating on PEEK significantly promoted cell adhesion and proliferation. And the osteogenic differentiation was effectively improved by BMP-2 gene releasing from PLGA-NH 2 microspheres. The results showed that this novel biological surface modification endowed PEEK with outstanding bioactivity and osteogenic ability, providing a theoretical basis for application in the field of implantation. K E Y W O R D S biomaterial, improved cell adhesion, PLGA-NH 2 microspheres, promoted osteogenic differentiation 1 | INTRODUCTION For repairing bone defects resulting from surgery, trauma, diseases, and congenital malformations, titanium-based alloys have become the Shuang Qin and Zhengkuan Lu contributed equally to this work

show abstract

“…Some scholars deposited silver nanoparticles on the PEEK surface by PDA-assisted deposition method, and good antibacterial effect was observed in vitro and in vivo experiments. [39][40][41][42]49 In addition, some scholars fixed silver ions on the HAp membrane through the chelating ability of inositol phosphate (IP6) to play an antibacterial role. 43,44 The inhibition ring experiment showed a prominent silver ions inhibition zone around the sample, and the size was positively correlated with the concentration.…”

Section: Silvermentioning

confidence: 99%

Modification strategies for improving antibacterial properties of polyetheretherketone

Gao

Han

et al. 2022

J of Applied Polymer Sci

View full text Add to dashboard Cite

Polyetheretherketone (PEEK) is a semi‐crystalline thermoplastic polymer that has been gradually used in the repair of bone defects because of its well chemical stability, reliable biocompatibility, excellent X‐ray radiolucency, and low modulus. However, PEEK is a biologically inert material, and planktonic bacteria can adhere to its surface and eventually form biofilms, allowing for the rapid development of implant‐related infections. This review mainly introduces two important antibacterial modification strategies: antibacterial coating and surface morphology modification. Antibacterial coating include antibiotics, natural extracts, antimicrobial peptides, polymers, metals and metal oxides, selenium, fluoride, silicon nitride, and graphene oxide. These coatings can exert their antibacterial effect by inhibiting bacterial adhesion, killing bacteria by contact, or actively releasing antimicrobial substances. In terms of antibacterial surface topography, different surface microstructures have their respective antibacterial functions. Conical or cylindrical structures can exert stress on the bacterial surface causing the rupture of the cell membrane to kill bacteria. Nano protrusions or particles smaller than the size of bacteria can inhibit the adhesion of bacteria by reducing the contact area of bacteria and PEEK. The pit structure close to the size of bacteria can prevent the contact between adjacent bacteria and delay or prevent the formation of biofilm.

show abstract

Ag and peptide co-decorate polyetheretherketone to enhance antibacterial property and osteogenic differentiation

Cited by 35 publications

References 28 publications

Facilitating GL13K Peptide Grafting on Polyetheretherketone via 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide: Surface Properties and Antibacterial Activity

Facilitating GL13K Peptide Grafting on Polyetheretherketone via 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide: Surface Properties and Antibacterial Activity

Construction of a BMP‐2 gene delivery system for polyetheretherketone bone implant material and its effect on bone formation in vitro

Modification strategies for improving antibacterial properties of polyetheretherketone

Contact Info

Product

Resources

About