CuO nano akes with dimension of 50-150 nm × 200 nm were successfully immobilized on the surface of cellulose bers by an ultrafast (5 min reaction time), scalable microwave assisted method. The achievement of high retention of CuO upto 70% has been phenomenal as the immobilization was carried out in the absence of any linker, binder or retention aid. The successful recyclability of the CuO immobilized paper matrices upto ve consecutive cycles while retaining 87% of immobilized CuO nanostructures in paper matrices has been unprecedented. The antimicrobial activities of the paper matrices have been investigated by deactivating both G. trabeum and E. coli . The CuO immobilized paper matrices have desisted the growth of G. trabeum for upto 28 days, which is phenomenal and completely deactivated E. coli of the CFU in order of 10 7 in the presence of visible light with exposure time of 2 h only.