Ag/MoO3–Pd-mediated gasochromic reaction: An efficient dual-mode photoelectrochemical and photothermal immunoassay

Lu, Liling; Hu, Xuehan; Zeng, Ruijin; Lin, Qianyun; Huang, Xue; Wei, Qing; Tang, Dianping; Knopp, Dietmar

doi:10.1016/j.bios.2023.115267

Cited by 41 publications

(10 citation statements)

References 32 publications

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“…Although the tremendous potential of polarity-switchable PEC sensing platforms has been certified, relying on only a single signal output might be influenced by operators, instruments, and measurement conditions. , For further improving the reliability and accuracy of the analytical protocols, various strategies with dual-mode, relatively independent signals generated by various response mechanisms have been proposed recently. For dual-readout strategies, the two relatively independent signals obtained are not only distinctive but also able to verify and support each other, which facilitates the acquisition of more accurate and diverse information in the field of point-of-care (POC) detection. − Up to now, the ingeniously developed dual-mode strategies with PEC signal as one readout involves PEC-colorimetric, PEC-photothermal, PEC-electrochemical, PEC-fluorescent dual-mode signals, etc. Among these dual-readout analyses, a dual-readout sensing platform combining PEC with photothermal performance is of great value.…”

Section: Introductionmentioning

confidence: 99%

Cation Exchange Reaction-Mediated Photothermal and Polarity-Switchable Photoelectrochemical Dual-Readout Biosensor

Lu,

Zeng,

Lin

et al. 2023

Anal. Chem.

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112

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Cation exchange (CE) is a burgeoning method for controlled crystal synthesis; however, its applications in bioanalysis are still in their infancy. Herein, we explored the transformation of ZnIn 2 S 4 in properties after the CE reaction with Cu 2+ ions; furthermore, the discrepancy was employed to design a dual-readout detection system of photothermal and polarity-switchable photoelectrochemical (PEC) immunoassays to realize reliable detection of carcinoembryonic antigen (CEA). In the presence of CEA, the CuO nanoparticles (CuO NPs) employed as dual-signal response probes would bond to the microplates and be acidolyzed by HCl to release Cu 2+ , which could replace Zn 2+ and In 3+ via the CE reaction. After the CE reaction is completed, the photocurrent would switch from a weak anodic photocurrent to a cathode one by using a 635 nm laser as a signal amplifier, while the photothermal signal would be enhanced with 808 nm laser illumination. On the basis of the polarity-switchable PEC strategy, CEA could be accurately detected from 0.1 to 50 ng mL −1 with a limit of detection (LOD) of 48 pg mL −1 (S/N = 3). Moreover, the photothermal assay for CEA detection possesses a linear range from 0.5 to 100 ng mL −1 with a LOD of 0.21 ng mL −1 . In addition, the designed sensing platform only relies on devices with portability that are permitted for point-of-care detection.

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Section: Introductionmentioning

confidence: 99%

Cation Exchange Reaction-Mediated Photothermal and Polarity-Switchable Photoelectrochemical Dual-Readout Biosensor

Lu,

Zeng,

Lin

et al. 2023

Anal. Chem.

Self Cite

112

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“…In order to improve the accuracy of the biosensing, dual-mode biosensors have spurred tremendous enthusiasm on account of their self-calibration function to exclude the influence of systematic error; the interverifiable dual-response generated by independent signal transduction endows the biosensor with self-calibration function, demonstrating higher reliability than single-mode system. − Notably, electrochemical (EC)/fluorescent (FL) dual-mode system has the advantages of simplicity and rapidity in EC measurement, while FL sensing adopts light as the output signal to increase sensitivity. − So far, most EC/FL dual-mode biosensors are manufactured by integrating two signal probes and relatively independent signal transduction patterns . Notwithstanding no interference between the two separate signaling routes, the adoption of diverse signal probes will bring inevitable blemishes during the construction of biosensors, such as cumbersome signal probe assembly and uncontrollable fluctuation in different batches .…”

Section: Introductionmentioning

confidence: 99%

Thermodynamic Allosteric Switch-Actuated 3D DNA Nanomachine for Ultrasensitive Electrochemical/Fluorescent Dual-Mode Biosensing of a Transcription Factor

Wang,

Zhang,

Liu

et al. 2024

ACS Appl. Bio Mater.

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Herein, we reported an innovative thermodynamic allosteric switch-actuated 3D DNA nanomachine for selective, sensitive, and accurate electrochemical (EC)/fluorescent (FL) dual-mode biosensing of a microphthalmia-associated transcription factor (MITF). The thermodynamic allosteric switch was ingeniously customized as a hairpin probe (HP) that was in dynamic equilibrium but rapidly interconverting conformations. At the "inactive state", the MITF-binding region and the switch part were "sequestered". Upon the introduction of MITF, an MITF-HP complex promptly formed, and the equilibrium of HP thermodynamically inclined from the "inactive state" toward the "active state" conformation. Immediately, the exposed switch on HP effectively actuated the 3D DNA nanomachine and synchronously produced the restriction site for Nb.BbvCI nicking endonuclease. After the autonomous conveying of the 3D DNA nanomachine by means of the high-efficiency circularly nicking endonuclease signal amplification (NESA), not only was MB-S1 in the supernatant used for FL measurements but also MB-SP/MNs/S2 in the precipitate was adapted for EC analysis, significantly improving the utilization of output products derived from the 3D DNA nanomachine. Accordingly, benefiting from the efficient DNA nanomachine signal amplification manner and the self-calibration function of a dual-mode bioassay, the constructed biosensor exhibits superior sensitivity and accuracy for MITF determination.

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“…Examples include photoelectrochemistry (PEC) and electrochemistry (EC), fluorescence (FL), and chemiluminescence (CL) . In recent years, dual-mode immunoassay platforms developed by combining the photothermal effects of nanomaterials have provided new ideas for the detection of disease biomarkers. − …”

Section: Introductionmentioning

confidence: 99%

Construction of a Dual-Mode Immune Platform Based on the Photothermal Effect of AgCo@NC NPs for the Detection of α-Fetoprotein

Wang,

Jiang,

et al. 2023

Anal. Chem.

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Compared with the accuracy of a single signal and the limitation of environmental applicability, the application value of dual-mode detection is gradually increasing. To this end, based on the photothermal effect of Ag/Co embedded N-rich mesoporous carbon nanomaterials (AgCo@NC NPs), we designed a dual-mode signal response system for the detection of α-fetoprotein (AFP). First, AgCo@NC NPs act as a photothermal immunoprobe that converts light energy into heat driven by a near-infrared (NIR) laser and obtains temperature changes corresponding to the analyte concentration on a hand-held thermal imager. In addition, this temperature recognition system can significantly improve the efficiency of Fenton-like reactions. AgCo@NC NPs act as peroxidase mimics to initiate the generation of poly N-isopropylacrylamide (PNIPAM, resistance enhancer) by cascade catalysis and the degradation of methylene blue (MB), thus enabling electrochemical testing. The dual-mode assay ranges from 0.01 to 100 and 0.001−10 ng/mL, with lower limits of detection (LOD) of 3.2 and 0.089 pg/mL, respectively, and combines visualization, portability, and high efficiency, opening new avenues for future clinical diagnostics and inhibitor studies.

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Ag/MoO3–Pd-mediated gasochromic reaction: An efficient dual-mode photoelectrochemical and photothermal immunoassay

Cited by 41 publications

References 32 publications

Cation Exchange Reaction-Mediated Photothermal and Polarity-Switchable Photoelectrochemical Dual-Readout Biosensor

Cation Exchange Reaction-Mediated Photothermal and Polarity-Switchable Photoelectrochemical Dual-Readout Biosensor

Thermodynamic Allosteric Switch-Actuated 3D DNA Nanomachine for Ultrasensitive Electrochemical/Fluorescent Dual-Mode Biosensing of a Transcription Factor

Construction of a Dual-Mode Immune Platform Based on the Photothermal Effect of AgCo@NC NPs for the Detection of α-Fetoprotein

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