Agar Gel Alkaline Electrophoresis As A Cost Effective Screening Modality For Abnormal Hemoglobins

Rentapalli, Babu Rao; Gade, Jayalakshmi; Sulemani, Dawood; Ganji, Suresh Babu; Thandu, Pavani Kiranmai; Revupalli, Suneetha; Kavali, Sudhakar; Ramtenki, Anitha

doi:10.9790/0853-1604083943

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…Initial recommended manual method of Alkaline gel electrophoresis can be used where cost is a limiting factor. 21 However the precision and accuracy of HbA2 measurements using densitometric scanning of electrophoretic gels is poor, especially when compared with HPLC techniques which showed a CV of 33.6% for densitometric scanning of electrophoretic gels at a HbA2 concentration of 2.41%. For column chromatography, the CV is 14.6% at a mean HbA2 concentration of 3.21%, and for HPLC, the CV is 4.3% at a mean HbA2 of 3.47%.…”

Section: Hemoglobin Electrophoresismentioning

confidence: 99%

Utility of basic hematological indices in early detection of hemoglobinopathies in paediatric population of southern India.

VM,

2023

J Pathol Nep

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Background: Cases diagnosed as Microcytic Hypochromic Anaemia in paediatric age group will be subjected to basic hematological indices and further confirmed in cases of high suspicion through Hemoglobin Electrophoresis and High-Performance Liquid Chromatography. This study aimed to identify simple indices and cost-effective methods to detect undiagnosed cases of thalassemia syndromes being treated for nutritional deficiency anemias. Materials and Methods: The study included 100 pediatric patients, from a period of January 2018 to June 2020. Case selection was based on children whose hemoglobin levels were below the cutoff value according to age. They were further subjected to Sehgal’s and Mentzer’s indices and suggestive cases were subjected to Gel Electrophoresis (Both Alkaline and Acid) and High-Performance Liquid Chromatography. Results: 43% of the cases showing abnormality on screening hematological indices were further subcategorized on gel electrophoresis and confirmed on HPLC. 27% of the cases were diagnosed to be β Thalassemia Trait, which constituted the most frequent type of hemoglobinopathy detected. There were 8% of the cases diagnosed to be as Sickle Cell disease which included major and minor forms. 5% of the study cases were diagnosed as β Thalassemia major. 3% of the cases were diagnosed as HbE disease, HbE trait and ȣβ Thalassemia. Conclusions: Hematological indices can be used effectively for early detection of suspected cases of hemoglobinopathies.

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Section: Hemoglobin Electrophoresismentioning

confidence: 99%

Utility of basic hematological indices in early detection of hemoglobinopathies in paediatric population of southern India.

VM,

2023

J Pathol Nep

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“…Hemoglobin electrophoresis can differentiate between HbS and HbC, which are the most clinically significant variants. However, electrophoresis does not distinguish between hemoglobin variants with the same electrical charges and gives the same migration patterns, such as HbD and HbG, which comigrate with HbS; HbE and Hb0-Arab have similar migration to the HbC molecules [ 30 ]. Furthermore, alkaline electrophoresis can be affected by the presence of large amounts of hemoglobin F in newborns, which can dominate the smaller electrophoresis band.…”

Section: Current Techniques To Diagnose and Monitor Scdmentioning

confidence: 99%

Techniques for the Detection of Sickle Cell Disease: A Review

2021

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Sickle cell disease (SCD) is a widespread disease caused by a mutation in the beta-globin gene that leads to the production of abnormal hemoglobin called hemoglobin S. The inheritance of the mutation could be homozygous or heterozygous combined with another hemoglobin mutation. SCD can be characterized by the presence of dense, sickled cells that causes hemolysis of blood cells, anemia, painful episodes, organ damage, and in some cases death. Early detection of SCD can help to reduce the mortality and manage the disease effectively. Therefore, different techniques have been developed to detect the sickle cell disease and the carrier states with high sensitivity and specificity. These techniques can be screening tests such as complete blood count, peripheral blood smears, and sickling test; confirmatory tests such as hemoglobin separation techniques; and genetic tests, which are more expensive and need to be done in centralized labs by highly skilled personnel. However, advanced portable point of care techniques have been developed to provide a low-cost, simple, and user-friendly device for detecting SCD, for instance coupling solubility tests with portable devices, using smartphone microscopic classifications, image processing techniques, rapid immunoassays, and sensor-based platforms. This review provides an overview of the current and emerging techniques for sickle cell disease detection and highlights the different potential methods that could be applied to help the early diagnosis of SCD.

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Sickle Cell Anemia and Related Hemoglobinopathies

Gupta

2024

Decision Making Through Problem Based Learning in Hematology

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Agar Gel Alkaline Electrophoresis As A Cost Effective Screening Modality For Abnormal Hemoglobins

Abstract: Alkaline agar gel electrophoresis is a basic manual laborious technique for identifying

Cited by 4 publications

References 12 publications

Utility of basic hematological indices in early detection of hemoglobinopathies in paediatric population of southern India.

Utility of basic hematological indices in early detection of hemoglobinopathies in paediatric population of southern India.

Techniques for the Detection of Sickle Cell Disease: A Review

Sickle Cell Anemia and Related Hemoglobinopathies

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