Agar Medium to Define Acid Producing Bacteria

Shank, J. L.; Silliker, J. H.

doi:10.1128/jb.73.5.625-626.1957

J Bacteriol

1957

DOI: 10.1128/jb.73.5.625-626.1957

|View full text |Cite

Agar Medium to Define Acid Producing Bacteria

J. L. Shank

J. H. Silliker

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1966

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

The Congo Red Reaction in Bacteria and Its Usefulness in the Identification of Rhizobia

Hahn¹

1966

Can. J. Microbiol.

View full text Add to dashboard Cite

Congo red was found in this research to form colloidal complexes with di-and tri-valent cations in the presence of acid and alkali. The complexes formed at a pH of 2–3 were uniformly blue; those formed at a pH greater than 12 varied in color with the ion involved, a fuchsia complex being formed with magnesium. The congo red reaction in bacteria (e.g. coloration of the growth in media containing the dye) is explained as due to the adsorption of Congo red on the surface of the bacterial cell, to the ions which predominate at the surface, and to the production of acid or alkali by the bacterium during growth. The temperature of incubation and composition of the growth medium influence colony coloration by affecting the metabolism of the organism.A negative form of selection for rhizobia is suggested using a nitrogen-deficient and a nitrogen-rich carbohydrate medium containing 0.25 g Congo red per liter of agar and 0.025 g Congo red per liter of broth, with incubation at 28 °C and 37 °C respectively. Color differentiation among the rhizobial strains tested, four normal and seven mutant varieties of Rhizobium meliloti and Rhizobium trifolii, occurred on the nitrogen-rich agar medium when incubation was carried out at 25 °C to 28 °C.

show abstract

The Congo Red Reaction in Bacteria and Its Usefulness in the Identification of Rhizobia

Hahn¹

1966

Can. J. Microbiol.

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Agar Medium to Define Acid Producing Bacteria

Cited by 1 publication

References 1 publication

The Congo Red Reaction in Bacteria and Its Usefulness in the Identification of Rhizobia

The Congo Red Reaction in Bacteria and Its Usefulness in the Identification of Rhizobia

Contact Info

Product

Resources

About