The paper provides the data on new records of 24 protected and two rare in St. Petersburg fungi, and on 15 basidiomycete species new to St. Petersburg (Amanita excelsa, Amylocystis lapponica, Antrodia hyalina, Dendrothele acerina, Granulobasidium vellereum, Hypochnicium wakefieldiae, Kneiffiella floccosa, Macrolepiota excoriata, Mycena erubescens, Psathyrella pygmaea, Rhizochaete sulphurina, Sidera vulgaris, Sistotrema raduloides, Skeletocutis stellae, Tricholoma matsutake). New data on mycobiota of some protected areas has been obtained. Amylocystis lapponica, Antrodia piceata, Skeletocutis stellae, Tricholoma matsutake are recommended for inclusion in the list of protected species of St. Petersburg.