Agarose-based microwell array chip for high-throughput screening of functional microorganisms

Zhang, Leicheng; Chen, Peng; Zhou, Zhuoyue; Hu, Yihong; Sha, Qiuyue; Zhang, Houjin; Liu, Xin; Du, Wei; Feng, Xiaojun; Liu, Bi‐Feng

doi:10.1016/j.talanta.2018.08.090

Cited by 19 publications

(16 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The throughput of the device can be further increased by decreasing the volume or diameter of the microwell if permitted by the desired bacterial assays. For example, some studies maximized the number of microwells by fabricating a picoliter- or femtoliter-scale microwell array for partitioning cells as much as possible . Although the reducing microwell volume improves the partitioning, the basic functions of microwells are still considered for potential application in analytical or biological assays such as injecting additional chemicals and errorless automated extraction.…”

Section: Results and Discussionmentioning

confidence: 99%

“…For example, some studies maximized the number of microwells by fabricating a picoliteror femtoliter-scale microwell array for partitioning cells as much as possible. 31 Although the reducing microwell volume improves the partitioning, the basic functions of microwells are still considered for potential application in analytical or biological assays such as injecting additional chemicals and errorless automated extraction. Because of the engineering trade-off, we adopted nanoliter-scale microwell arrays and then made a breakthrough in this study.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Double-Sided Microwells with a Stepped Through-Hole Membrane for High-Throughput Microbial Assays

Bae

Kim

et al. 2020

Anal. Chem.

View full text Add to dashboard Cite

To improve the throughput of microwell arrays for identifying immense cellular diversities even at a single-bacteria level, further miniaturization or densification of the microwells has been an obvious breakthrough. However, controlling millions of nanoliter samples or more at the microscale remains technologically difficult and has been spatially restricted to a single open side of the microwells. Here we employed a stepped through-hole membrane to utilize the bottom as well as top side of a high-density nanoliter microwell array, thus improving spatial efficiency. The stepped structure shows additional effectiveness for handling several millions of nanoliter bacterial samples in the overall perspectives of controllability, throughput, simplicity, versatility, and automation by using novel methods for three representative procedures in bacterial assays: partitioning cells, manipulating the chemical environment, and extracting selected cells. As a potential application, we show proof-of-concept isolation of rare cells in a mixed ratio of 1 to around 106 using a single chip. Our device can be further applied to various biological studies pertaining to synthetic biology, drug screening, mutagenesis, and single-cell heterogeneity.

show abstract

Section: Results and Discussionmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Double-Sided Microwells with a Stepped Through-Hole Membrane for High-Throughput Microbial Assays

Bae

Kim

et al. 2020

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…Furthermore, Inoue et al [ 5 ] have studied the Escherichia coli division growth at the single‐cell level by creating micro‐chambers from microwells. Lastly, Zhang et al [ 154 ] used an agarose‐based microwell array chip for the isolation of the lipolytic enzyme expressing bacteria, which was further utilized for the screening of other enzyme activities with suitable fluorogenic substrates. They proposed that this agarose‐based microwell chip would be useful for high‐throughput screening analysis due to the less reagent consumption and reduced operational time.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

A review on microwell and microfluidic geometric array fabrication techniques and its potential applications in cellular studies

2020

View full text Add to dashboard Cite

The ability to trap precise quantities of cells or particles into confined areas has numerous applications for biological purposes. In particular, single cell trapping has received considerable attention because it permits the study of heterogeneity in a population, while trapping larger groups of cells have been used to form aggregates. Among several methods, the use of microwell offers a simple platform to capture cells or particles using hydrodynamic forces. This review examines the use of microwells in both static and microfluidic environments, and the application of microfluidic geometric arrays for trapping. This paper discusses the design and fabrication methods of microwells and compares the trapping and release techniques used in both static and microfluidicsintegrated microwells. Finally, we will summarize novel microfluidic geometric arrays used to capture cells or particles through hydrodynamic trapping.

show abstract

“…Many researchers proposed several approaches to minimize cell loss in the developed microwell arrays and to allow the efficient formation of 3D culture products until recently [1, 13, 21–35]. In this study, we describe a 3D MCTs formation method using novel concave microwell arrays with a cell-loss-free (CLF) platform.…”

Section: Introductionmentioning

confidence: 99%

A cell-loss-free concave microwell array based size-controlled multi-cellular tumoroid generation for anti-cancer drug screening

et al. 2019

View full text Add to dashboard Cite

The 3D multi-cellular tumoroid (MCT) model is an in vivo -like, avascular tumor model that has received much attention as a refined screening platform for drug therapies. Several types of research have been efforted to improve the physiological characteristics of the tumor microenvironment (TME) of the in vivo -like MCTs. Size-controlled MCTs have received much attention for obtaining highly reproducible results in drug screening assays and achieving a homogeneous and meaningful level of biological activities. Here, we describe an effective method for fabricating the size-controlled in vivo -like MCTs using a cell-loss-free (CLF) microwell arrays. The CLF microwell arrays was fabricated by using the simple operation of laser carving of a poly (methyl methacrylate) (PMMA) master mold. We also demonstrated the biophysicochemical effect of tumor microenvironment (TME) resident fibroblasts through the expression of TGFβ, αSMA, Type I-, IV collagen, angiogenesis related markers on tumorigenesis, and confirmed the drug response of MCTs with anti-cancer agents. This technology for the fabrication of CLF microwell arrays could be used as an effective method to produce an in vitro tumor model for cancer research and drug discovery.

show abstract

Agarose-based microwell array chip for high-throughput screening of functional microorganisms

Cited by 19 publications

References 39 publications

Double-Sided Microwells with a Stepped Through-Hole Membrane for High-Throughput Microbial Assays

Double-Sided Microwells with a Stepped Through-Hole Membrane for High-Throughput Microbial Assays

A review on microwell and microfluidic geometric array fabrication techniques and its potential applications in cellular studies

A cell-loss-free concave microwell array based size-controlled multi-cellular tumoroid generation for anti-cancer drug screening

Contact Info

Product

Resources

About