Agarose-Degrading Characteristics of a Deep-Sea Bacterium Vibrio Natriegens WPAGA4 and Its Cold-Adapted GH50 Agarase Aga3420

Zhang, Mengyuan; Wang, Jianxin; Zeng, Runying; Wang, Dingquan; Wang, Wenxin; Tong, Xiufang; Qu, Wu

doi:10.3390/md20110692

Cited by 7 publications

(2 citation statements)

References 69 publications

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“… Cao et al [ 46 ] Enzymatic modification GH50 Agarosease Aga3420 Efficient production of high purity neoagarobiose (NA2) in a low temperature environment. Zhang et al [ 47 ] Physical modification Electronic irradiation Material properties of agarose hydrogels can be adjusted at a low doses of high energy electron irradiation. Krömmelbein et al [ 41 ] Ultrasound Drug delivery 1-MHz Ultrasound High frequency ultrasound sonication enhances the internal diffusivity of agarose gels and aid drug delivery.…”

Section: Extraction and Modification Of Agarosementioning

confidence: 99%

Extraction, Modification and Biomedical Application of Agarose Hydrogels: A Review

Jiang

Chen

et al. 2023

Marine Drugs

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Numerous compounds present in the ocean are contributing to the development of the biomedical field. Agarose, a polysaccharide derived from marine red algae, plays a vital role in biomedical applications because of its reversible temperature-sensitive gelling behavior, excellent mechanical properties, and high biological activity. Natural agarose hydrogel has a single structural composition that prevents it from adapting to complex biological environments. Therefore, agarose can be developed into different forms through physical, biological, and chemical modifications, enabling it to perform optimally in different environments. Agarose biomaterials are being increasingly used for isolation, purification, drug delivery, and tissue engineering, but most are still far from clinical approval. This review classifies and discusses the preparation, modification, and biomedical applications of agarose, focusing on its applications in isolation and purification, wound dressings, drug delivery, tissue engineering, and 3D printing. In addition, it attempts to address the opportunities and challenges associated with the future development of agarose-based biomaterials in the biomedical field. It should help to rationalize the selection of the most suitable functionalized agarose hydrogels for specific applications in the biomedical industry.

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Section: Extraction and Modification Of Agarosementioning

confidence: 99%

Extraction, Modification and Biomedical Application of Agarose Hydrogels: A Review

Jiang

Chen

et al. 2023

Marine Drugs

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“…Unfortunately, the contribution of HGT to the pathway of agarose biodegradation in deep‐sea strains has been rarely studied until now. In previous work, we have studied the enzyme activity properties and enzyme activities of the four agarases obtained from Vibrio natriegens WPAGA4 from deep‐sea sediments [27, 28]. In current work, the phylogeny and expression level of agarases, degradation products, and GI in the genome of V. natriegens WPAGA4 was studied to reveal the agarose degradation pathway and the importance of HGT for the agarose degradation in V. natriegens WPAGA4, thereby widening our understanding of agarose utilization in deep‐sea environments.…”

Section: Introductionmentioning

confidence: 99%

Agarose biodegradation by deep‐sea bacterium Vibrio natriegens WPAGA4 with the agarases through horizontal gene transfer

Zhang,

Tong,

Wang

et al. 2023

J Basic Microbiol

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This study aimed to reveal the importance of horizontal gene transfer (HGT) for the agarose‐degrading ability and the related degradation pathway of a deep‐sea bacterium Vibrio natriegens WPAGA4, which was rarely reported in former works. A total of four agarases belonged to the GH50 family, including Aga3418, Aga3419, Aga3420, and Aga3472, were annotated and expressed in Escherichia coli cells. The agarose degradation products of Aga3418, Aga3420, and Aga3472 were neoagarobiose, while those of Aga3419 were neoagarobiose and neoagarotetraose. The RT‐qPCR analysis showed that the expression level ratio of Aga3418, Aga3419, Aga3420, and Aga3472 was stable at about 1:1:1.5:2.5 during the degradation, which indicated the optimal expression level ratio of the agarases for agarose degradation by V. natriegens WPAGA4. Based on the genomic information, three of four agarases and other agarose‐degrading related genes were in a genome island with a G + C content that was obviously lower than that of the whole genome of V. natriegens WPAGA4, indicating that these agarose‐degrading genes were required through HGT. Our results demonstrated that the expression level ratio instead of the expression level itself of agarase genes was crucial for agarose degradation by V. natriegens WPAGA4, and HGT occurred in the deep‐sea environment, thereby promoting the deep‐sea carbon cycle and providing a reference for studying the evolution and transfer pathways of agar‐related genes.

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Agarose Degrading Potential and Whole Genome Sequence Analysis of Marine Bacterium Aliagarivorans sp. Strain DM1 Isolated from the Arabian Sea

Kumar,

Meenakshi,

Chaudhary

2024

Curr Microbiol

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Agarose-Degrading Characteristics of a Deep-Sea Bacterium Vibrio Natriegens WPAGA4 and Its Cold-Adapted GH50 Agarase Aga3420

Cited by 7 publications

References 69 publications

Extraction, Modification and Biomedical Application of Agarose Hydrogels: A Review

Extraction, Modification and Biomedical Application of Agarose Hydrogels: A Review

Agarose biodegradation by deep‐sea bacterium Vibrio natriegens WPAGA4 with the agarases through horizontal gene transfer

Agarose Degrading Potential and Whole Genome Sequence Analysis of Marine Bacterium Aliagarivorans sp. Strain DM1 Isolated from the Arabian Sea

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