Agarose gel serum protein electrophoresis in cats with and without lymphoma and preliminary results of tandem mass fingerprinting analysis

Gerou-Ferriani, Magda; McBrearty, Alix; Burchmore, Richard; Jayawardena, K. G. I.; Eckersall, P.D.; Morris, Joanna S.

doi:10.1111/j.1939-165x.2011.00310.x

Cited by 23 publications

(20 citation statements)

References 26 publications

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“…Because dysproteinemia is associated with several diseases, it is important to determine the differences in protein electrophoretograms between healthy animals and cases affected by various diseases. Serum protein electrophoresis has been intensively studied in small animals medicine [4,5]. However, published reports about the changes in serum protein fractions in farm animals, including cows affected by some frequent diseases as mastitis, metritis and hoof diseases are very limited.…”

Section: Introductionmentioning

confidence: 99%

The electrophoretic pattern of serum proteins in dairy cows with inflammatory diseases

2017

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The objective of the study was to evaluate the electrophoretic pattern of serum proteins in dairy cows suffering from various infl ammatory diseases, and to study the infl uence of these diseases on the concentrations of protein fractions. Fourty dairy cows with clinical signs of various infl ammatory diseases were used in the study, including cows with post-partum metritis (n=10), mastitis (n=7), and hoof diseases (n=23). The cows were of a black pied Holstein-Friesian breed, Slovak spotted breed and their crossbreeds at the age of 3.5 to 8 years. Fourteen clinically healthy dairy cows were taken as the control group. The cows were blood sampled for the determination of total serum proteins and serum protein fractions. The protein fractions were divided into albumin, α 1 -, α 2 -, β 1 -, β 2 -, and γ-globulins. In cows with post-partum metritis we found signifi cantly lower concentrations of albumin (P<0.001) and signifi cantly higher values of α 1 -globulins (P<0.01) compared with healthy animals. Signifi cantly higher concentrations of β 1 -globulins were observed in cows with mastitis (P<0.001), while the γ-globulin fraction was non-signifi cantly higher. In cows with hoof diseases we recorded signifi cantly lower values of albumin (P<0.001), signifi cantly higher concentrations of α 1 -and β 1 -globulins (P<0.001), and non-signifi cantly higher β 2 -and γ-globulins. Moreover, in the electrophoretic pattern of serum proteins we found β-γ bridging in thirteen cows with hoof diseases. Presented data suggest marked infl uence of infl ammatory diseases on the concentrations of serum protein fractions in dairy cows. However, further investigations are needed to establish the diagnostic utility of serum protein electrophoresis in bovine clinical practice in relation to infl ammatory diseases.

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Section: Introductionmentioning

confidence: 99%

The electrophoretic pattern of serum proteins in dairy cows with inflammatory diseases

2017

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“…In human diagnostics, characteristic electrophoresis patterns have been described for numerous cases, including acute and chronic inflammation and malignant tumours (16). Protein electrophoresis has been widely used as a diagnostic test in human and veterinary medicine for over 40 years (7).…”

Section: Introductionmentioning

confidence: 99%

“…For this purpose, the sample is stained to reveal characteristic bands whose width and intensity of colour provide information about the amount of protein in a given fraction (49). This is considerably facilitated by computer analysis of the image (16). The result obtained is the percentage share of each fraction in the sample and the output is a characteristic graph enabling visual analysis of the result.…”

Section: Introductionmentioning

confidence: 99%

Application of native agarose gel electrophoresis of serum proteins in veterinary diagnostics

Jania

Andraszek

2016

Journal of Veterinary Research

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Electrophoretic techniques, used to separate mixtures of electrically charged particles, are widely used in science. One of these techniques, native protein electrophoresis in an agarose gel, is applied in human and veterinary medicine. Changes in the proportions of individual protein fractions correspond to significant changes in the physiology of the body. Although the pattern obtained by electrophoretic separation rarely indicates a specific disease, it provides valuable information for the differential diagnosis. Decades of research on the types of patterns obtained in the case of particular diseases have led to the accumulation of substantial knowledge. The paper presents the available information on this topic. Serum protein electrophoresis is recommended in cases of increased levels of total protein in order to reveal the nature of the process. The basic information which can be obtained from electrophoretic separation includes the immune status of the organism. Both increased antigenic stimulation and immunodeficiency are clearly visible in electropherograms. Moreover, the level of heterogeneity of the corresponding protein fractions can help to distinguish between infectious diseases and cancer -multiple myeloma -the latter producing a homogeneous immunoglobulin fraction. Analysis of other protein fractions helps to detect or confirm an ongoing inflammatory process and provides information regarding liver function. Even when the concentration of total protein is within the reference range, this analysis can be recommended as a basic laboratory test.

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“…In routine electrophoresis, serum cat proteins are separated into albumin, α 1 -, α 2 -, β 1 -, β 2 -, and γ-globulin fractions (Baker and Valli 1988). However, according to Gerou-Ferriani et al (2011), the globulin fractions in normal cats may be further divided into α 1a -, α 1b -, α 2a -, α 2b -, β 1 -and β 2 -subzones. In healthy horses, some authors (Kohn 1957;Riond et al 2009;Gundasheva 2015) clearly separated on the serum protein electrophoretogram six different bands, including albumin, α 1 -and α 2 -globulins, β 1 -and β 2 -globulins, and γ-globulins.…”

Section: Normal Serum Protein Patternmentioning

confidence: 99%

Serum proteins and their diagnostic utility in veterinary medicine: a review

Tóthová¹,

Nagy²,

Kováč³

2016

Vet. Med.

134

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ABSTRACT:The evaluation of serum proteins and their electrophoretic pattern is a well-established laboratory method in the diagnosis of many diseases in humans, which has replaced the biochemical determination of the concentrations of albumin and the ratio of albumin to globulins. The measurement of serum proteins may be an important diagnostic tool for the detection, diagnosis, and monitoring of various diseases and pathological processes. The results of serum protein electrophoresis can be one of the most useful diagnostic aids in a wide spectrum of diseases, including infectious and inflammatory diseases, renal or gastrointestinal disorders, immunodeficiency states, as well as paraproteinaemias caused by lymphoid or plasma cell neoplasia. Although many studies have been carried out to determine the usefulness of the determination of serum proteins and their electrophoretic pattern in various disease conditions and disorders also in animals, serum protein evaluation is still a relatively little-used diagnostic tool in veterinary medicine. In this article, methods of serum protein determination, their diagnostic utility in animal care practice and their different patterns in dysproteinaemias and paraproteinaemias are reviewed.

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Agarose gel serum protein electrophoresis in cats with and without lymphoma and preliminary results of tandem mass fingerprinting analysis

Cited by 23 publications

References 26 publications

The electrophoretic pattern of serum proteins in dairy cows with inflammatory diseases

The electrophoretic pattern of serum proteins in dairy cows with inflammatory diseases

Application of native agarose gel electrophoresis of serum proteins in veterinary diagnostics

Serum proteins and their diagnostic utility in veterinary medicine: a review

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