Age and Intrinsic Fitness Affect the Female Rotator Cuff Tendon Tissue

Thierbach, Manuela; Heyne, E.; Schwarzer, Michael; Koch, Lauren G.; Britton, Steven L.; Wildemann, Britt

doi:10.3390/biomedicines10020509

Cited by 3 publications

(1 citation statement)

References 55 publications

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“…All primer sequences except the scleraxis primer (ordered from Qiagen, Cat No. : QT01596028) were designed using Primer3 (Version 0.4.0) and NetPrimer software and produced by TIB Molbiol, Berlin, Germany; sequences are as described previously [ 34 ]. The 18s rRNA gene was chosen as the housekeeping gene.…”

Section: Methodsmentioning

confidence: 99%

Wrap It! Preventive Antimicrobial Treatment Shows No Negative Effects on Tenocytes and Tendons—A Comprehensive Approach

Thierbach

Müller

Stange

et al. 2023

JCM

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Although the rate of infection after the reconstruction of a ruptured anterior cruciate ligament (ACL) is low, prophylactic incubation of the graft with vancomycin (Vanco-wrap or vancomycin soaking) is routinely performed. A cytotoxic effect of vancomycin is reported for several cell types, and the prophylactic treatment might prevent infection but harm the tissue and cells. Aim: A comprehensive study was performed to investigate the effect of vancomycin on tendon tissue and isolated tenocytes using cell viability, molecular and mechanical analysis. Material and methods: Rat tendons or isolated tenocytes were incubated in increasing concentrations of vancomycin (0–10 mg/mL) for different times, and cell viability, gene expression, histology and Young’s modulus were analyzed. Results: The clinically used concentration of vancomycin (5 mg/mL for 20 min) had no negative effect on cell viability in the tendons or the isolated tenocytes, while incubation with the toxic control significantly reduced cell viability. Increasing the concentration and prolonging the incubation time had no negative effect on the cells. The expression of Col1a1, Col3a1 and the tenocyte markers mohawk, scleraxis and tenomodulin was not affected by the various vancomycin concentrations. The structural integrity as measured through histological and mechanical testing was not compromised. Conclusion: The results proved the safe application of the Vanco-wrap on tendon tissue. Level of evidence: IV.

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Section: Methodsmentioning

confidence: 99%

Wrap It! Preventive Antimicrobial Treatment Shows No Negative Effects on Tenocytes and Tendons—A Comprehensive Approach

Thierbach

Müller

Stange

et al. 2023

JCM

Self Cite

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Tough and anisotropic Janus hydrogel for tendon injury repair with controlled release of bFGF in tendon microenvironment

Jiang,

Zhu,

et al. 2024

Chemical Engineering Journal

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Decellularization of Dense Regular Connective Tissue—Cellular and Molecular Modification with Applications in Regenerative Medicine

Data,

Kulus,

Ziemak

et al. 2023

Cells

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Healing of dense regular connective tissue, due to a high fiber-to-cell ratio and low metabolic activity and regeneration potential, frequently requires surgical implantation or reconstruction with high risk of reinjury. An alternative to synthetic implants is using bioscaffolds obtained through decellularization, a process where the aim is to extract cells from the tissue while preserving the tissue-specific native molecular structure of the ECM. Proteins, lipids, nucleic acids and other various extracellular molecules are largely involved in differentiation, proliferation, vascularization and collagen fibers deposit, making them the crucial processes in tissue regeneration. Because of the multiple possible forms of cell extraction, there is no standardized protocol in dense regular connective tissue (DRCT). Many modifications of the structure, shape and composition of the bioscaffold have also been described to improve the therapeutic result following the implantation of decellularized connective tissue. The available data provide a valuable source of crucial information. However, the wide spectrum of decellularization makes it important to understand the key aspects of bioscaffolds relative to their potential use in tissue regeneration.

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Age and Intrinsic Fitness Affect the Female Rotator Cuff Tendon Tissue

Cited by 3 publications

References 55 publications

Wrap It! Preventive Antimicrobial Treatment Shows No Negative Effects on Tenocytes and Tendons—A Comprehensive Approach

Wrap It! Preventive Antimicrobial Treatment Shows No Negative Effects on Tenocytes and Tendons—A Comprehensive Approach

Tough and anisotropic Janus hydrogel for tendon injury repair with controlled release of bFGF in tendon microenvironment

Decellularization of Dense Regular Connective Tissue—Cellular and Molecular Modification with Applications in Regenerative Medicine

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