Age and metabolic risk factors associated with oxidatively damaged DNA in human peripheral blood mononuclear cells

Løhr, Mille; Jensen, Annie Aarup; Eriksen, Louise; Grønbæk, Morten; Loft, Steffen; Möller, Peter

doi:10.18632/oncotarget.3202

Cited by 36 publications

(28 citation statements)

References 55 publications

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“…Moreover, body weight loss resulted in a reduction in the level of DNA damage [104][105][106][107]. The amount of DNA with oxidative damage was associated with levels of cholesterol, triglycerides and HbA1c [108]. In the present study a relationship between DNA damage and both, total cholesterol and LDLcholesterol was also observed.…”

Section: Discussionsupporting

confidence: 70%

TNF-α G-308A genetic variants, serum CRP-hs concentration and DNA damage in obese women

Włodarczyk

Ciebiera²

2019

Mol Biol Rep

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Obesity is associated with inflammation, which can disturb genome stability. Tumor necrosis factor (TNF-α) polymorphism was found to affect TNF-α protein production and inflammation. Therefore, the present study illustrates the relationship between TNF-α polymorphism, the degree of inflammation assessed by serum high sensitivity C-reactive protein concentration (CRP-hs) and basal DNA damage in patients with obesity (BMI 30-34.9 kg/m 2) and control subjects with proper body mass (BMI < 25 kg/m 2). A total of 115 participants (75 obese premenopausal women; and 40 age-, and gender-matched controls) were included. Biochemical parameters (serum concentrations of total-cholesterol, HDL-cholesterol, LDL-cholesterol, triglycerides, glucose, apolipoprotein AI, CRP-hs) and endogenous DNA damage (determined by comet assay) were measured. TNF-α G-308A polymorphism (rs1800629) was analyzed by PCR-RFLP (PCR-restriction fragments length polymorphism). An effect of TNF-α genotype on serum CRP-hs concentration was noted (p = 0.031). In general, carriers of the rare A allele of the TNF-α G-308A polymorphism had significantly lower endogenous DNA damage and serum CRPhs concentrations than GG homozygotes, however, the protective effect of the A allele was especially visible in non-obese women. Serum CRP-hs concentrations and levels of DNA damage (% DNA in tail) were significantly higher in obese than in controls (p = 0.001 and p < 0.0001, respectively). The adjusted multiple linear regression analyses revealed a significant, independent impact of obesity on DNA damage (p = 0.00000) and no effect of other covariates i.e. age, TNF-α genotype and serum CRP-hs concentration. Our study showed that obesity has a significant impact on the levels of endogenous DNA damage. Obesity abolished the protective effect of A allele of the TNF-α G-308A polymorphism on DNA damage and on inflammation development observed in non-obese A allele carriers.

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Section: Discussionsupporting

confidence: 70%

TNF-α G-308A genetic variants, serum CRP-hs concentration and DNA damage in obese women

Włodarczyk

Ciebiera²

2019

Mol Biol Rep

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“…Lohr et al also confirmed this finding. Authors of that study indicated that the blood pressure was not associated with the oxidatively damaged DNA levels among individuals with MetS (23). By contrast, Najarzadeh et al (24) and Bitla et al (19) showed a significant correlation among these variables.…”

Section: Discussionmentioning

confidence: 90%

“…Age increase especially in women can elevate the risk of OxS (1,5,23). Previous data also revealed higher protein oxidation level in women compared to men.…”

Section: Mda Tac ----------------------------------------------------mentioning

confidence: 98%

Association of lipid peroxidation and antioxidant status with metabolic syndrome in Iranian healthy elderly women

et al. 2017

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Abstract.The interconnection between aging and metabolic syndrome (MetS) and their effect on oxidative stress (OxS) status lacks adequate information. Additionally, the agerelated changes of antioxidant defenses and OxS in senior women with MetS in comparison to healthy senior women are not yet established. We analyzed the correlation between oxidative defense status and OxS with MetS components. Through further examination of MetS and aging, we aimed to determine their independent effects on OxS and oxidative defense status. This community-based cross-sectional study was conducted in the rural area of Babol, Iran. A total of 75 women of ≥60 years of age with MetS along with 89 women with similar conditions without the MetS, serving as the control group, were studied. Blood glucose, lipid profile, malondialdehyde (MDA) and total antioxidant capacity (TAC) were determined. Data were analyzed using multiple linear regression, ANOVA and independent t-tests. MDA and TAC levels independently showed a significant correlation with triglyceride (TG), waist circumference, fasting blood glucose and high-density lipoprotein cholesterol (HDL-C). As suggested by the standardized B (0.810, -0.783, P<0.001; -0.052, P<0.001, 0.047, P<0.01), TG followed by HDL-C were the most strongly correlated factors with MDA and TAC. Furthermore, MetS and age were independent risk factors for antioxidant activity reduction and OxS. However, MetS had a much higher predictive power than age (standardized B 0.573 for MetS and 0.376 for age, P<0.001). Aging and MetS, both lead to OxS, but the impact of MetS on this disorder was far greater than the effect of age. However, their cumulative effects can lead to a worsening of the situation.Therefore, early diagnosis and treatment of MetS, especially in the elderly can prevent any adverse impact of MetS.

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“…We usually find substantially higher levels of FPG-sensitive sites than of hOGG1-sensitive sites in human PBMC samples, although the levels of FPG-and hOGG1-sensitive sites are similar and high after treatment with the photosensitizer Ro19-8022 or potassium bromate inducing specifically 8-oxodG [16][17][18].…”

Section: Collection and Analysis Of Biomarker Samplesmentioning

confidence: 84%

Controlled exposure to diesel exhaust and traffic noise – Effects on oxidative stress and activation in mononuclear blood cells

Hemmingsen

Möller

Jantzen

et al. 2015

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

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Age and metabolic risk factors associated with oxidatively damaged DNA in human peripheral blood mononuclear cells

Cited by 36 publications

References 55 publications

TNF-α G-308A genetic variants, serum CRP-hs concentration and DNA damage in obese women

TNF-α G-308A genetic variants, serum CRP-hs concentration and DNA damage in obese women

Association of lipid peroxidation and antioxidant status with metabolic syndrome in Iranian healthy elderly women

Controlled exposure to diesel exhaust and traffic noise – Effects on oxidative stress and activation in mononuclear blood cells

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