The study focused on gender pairing and results feedback of the cooperative behavior of college students. Randomly selected 84 college students, Males and females equally divided, using 3 (male-male pairing, male-female pairing and female-female pairing) multiply by 2 (feedback, no feedback results) completely randomized design, the dependent variable indicators was cooperation behavior frequency. The results showed that: (1) the cooperative behavior of university students on gender pairing had a significant difference; the frequency of cooperative behavior was higher in the case of female. (2)The results feedback affect cooperative behavior frequency, results feedback of the average cooperative behavior frequency was higher than no results feedback. (3)The interaction of gender pairing factors and results feedback was not significant.