Age- and Sex-related changes of peritoneal free cells in mice: Quantitative morphologic study.

Honma, Sato; Abe, Kazuhiro; Ito, Takashi

doi:10.1679/aohc1950.43.127

Cited by 3 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Coulter counter and channelyzer analysis: The total number and size distribution curve of pleural cells were obtained by a Coulter counter and channelyzer (Model ZBI) as previously described in detail (ABE et al, 1979a;HoNMA et al, 1980). For the purpose of comparison, the peritoneal cells were also dealt with in the same way.…”

Section: Methodsmentioning

confidence: 99%

“…However, the peritoneal cavity contains not only macrophages but also other kinds of free cells. In the mouse, as has been reported previously, peritoneal free cells are composed mainly of three kinds of cells; small lymphocytes, medium-sized mononuclear cells and macrophages (ABE et al, 1979a, b;HONMA et al, 1980). The pleural cavity, like the peritoneal cavity, is generally considered to contain macrophages ( VAN FURTH, 1976), but little information is available about its normal cellular constituents.…”

mentioning

confidence: 92%

See 1 more Smart Citation

Pleural free cells in the mouse: Quantitative and qualitative cell morphology.

Honma

Abe

Ito

1982

Arch. Histol. Jap., Arch. Histol. Jpn

Self Cite

View full text Add to dashboard Cite

Summary. Free cells in the pleural cavity of the mouse were studied by quantitative and qualitative morphological procedures.The size distribution curve of the cells, obtained by a Coulter counter and channelyzer, indicated that the pleural free cells were composed mainly of two populations, which were morphologically classified into small and large types. The small cells were about twice as numerous in females as in males, and the large type of cells exhibited no significant difference in number between the two sexes. Therefore, the total number of pleural cells was significantly greater in females than in males.The small pleural cells were similar in cytological features to the peritoneal mediumsized mononuclear cells described in previous papers (ABE et a!., 1979a, b) with the exception of their size. Typical small lymphocytes were also included among the small type of pleural cells, but they were very few in number. The large type pleural cells were macrophages. The pleural macrophages were somewhat variable in cell features, particularly in surface configuration, as compared with peritoneal macrophages.

show abstract

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 92%

Pleural free cells in the mouse: Quantitative and qualitative cell morphology.

Honma

Abe

Ito

1982

Arch. Histol. Jap., Arch. Histol. Jpn

Self Cite

View full text Add to dashboard Cite

show abstract

Peritoneal cells in mice: Quantitative and qualitative cell morphology

1979

View full text Add to dashboard Cite

Peritoneal free cells from 86 adult mice of both sexes were studied quantitatively and qualitatively.Peritoneal free cells were examined by use of a Coulter counter and channelyzer. The size-distribution curves of peritoneal free cells thus obtained show three peaks, which were almost constant in position in all the cases examined. Thus, peritoneal free cells can be classified into three major populations according to cell size. The total cell numbers of the three populations, composed of small, medium-sized and large cells are, on the average, 1.60 x lo6, 2.47 x lo6 and 2.90 x lo6 in males and 2.05 x lo6, 5.57 x lo6 and 3.86 x lo6 in females a t 65 days of age. The number of medium-sized cells is significantly greater in females than in males.Morphologically, three types of peritoneal cells, which constitute the three populations, can be distinguished cytologically and cytochemically, when examined by light microscopy and scanning and transmission electron microscopy. These type I, 11, and 111 cells correspond closely to the three components in the size distribution curves in cell size.Type I cells are small lymphocytes. Type I1 cells are medium-sized, round mononuclear cells which are distinguishable from lymphocytes by their ultrastructural characteristics, and they constitute a major population which can be separated from the other two populations. Type I11 cells are macrophages. In the peripheral cytoplasm, they have a labyrinth of tubules which open on the cell surface.Macrophages have recently attracted much attention in relation to immune response (van Furth, '75; Nelson, '76). The peritoneal cavity is known to contain so many free macrophages that macrophages are generally obtained from the peritoneal cavity for experimental study (Stuart et al., '73). However, the peritoneal cavity contains other kinds of free cells in addition to macrophages (Felix and Dalton, '55; Padawer and Gordon, '56; Bennett, '65; Carr, '67; Davis and McGowan, '68; Gallily and Ben-Ishay, '74; Daems and Koerten, '78). Therefore, exact knowledge of free cells in the peritoneal cavity is needed for studies using peritoneal free cells. However, very little is known about cellular constituents other than macrophages in the peritoneal cavity (Catanzaro and Graham, '74; Gallily and Ben-Ishay, '74). In this study we attempted to examine the peritoneal free cells qualitatively and quantitatively. MATERIALS AND METHODSA total of 86 mice of strain dd, of both sexes, ranging in age from 40 to 65 days, were used. The mice were deeply anesthesized with chloroform, and 3-5 ml of one of the following fixing fluids was injected into the peritoneal cavity: 5% formalin in 0.1 M phosphate buffer (pH 7.5) or 2% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.5). After the peritoneal cavity was gently shaken or prodded, the total volume of the injected fluid containing peritoneal free cells was aspirated and examined in the following ways.Total number and size-distribution of peritoneal free cells were obtained with a Coulter counter (Model ZB...

show abstract

Effects of hydrocortisone on peritoneal free cells in mice.

Honma

Abe

Ito

1981

Arch. Histol. Jap., Arch. Histol. Jpn

View full text Add to dashboard Cite

Effects of hydrocortisone on peritoneal free cells in the mouse were examined by quantitative morphologic procedures. Mice of both sexes at 60 to 65 days of age received one, two, or four successive subcutaneous injections of 0.5mg hydrocortisone every 24hrs. After one injection, peritoneal free cells showed a rapid decrease in number during the first 3hrs. They then increased up to about 1.5 times the control value at 24hrs and returned to the normal level at 48hrs in both sexes. After two injections, peritoneal cells showed a significant decrease in number in females during the first 2 days. Thereafter they returned to normal in both sexes at 8 to 12 days. The three major types of peritoneal cells, type I, II and III cells, which have been described in pervious papers (ABE et al., 1979a, b; HONMA et al., 1980), differed in response to hydrocortisone. Type I cells (small lymphocytes) were markedly reduced in number immediately after hydrocortisone injection and remained depleted even 12 days after injection. Type II cells (medium-sized mononuclear cells) showed the most remarkable response to hydrocortisone. Changes in the total number of peritoneal cells following hydrocortisone injection were ascribed mainly to those of type II cells. Type III cells (macrophages) did not show any significant changes in number after injection.

show abstract

Age- and Sex-related changes of peritoneal free cells in mice: Quantitative morphologic study.

Cited by 3 publications

References 22 publications

Pleural free cells in the mouse: Quantitative and qualitative cell morphology.

Pleural free cells in the mouse: Quantitative and qualitative cell morphology.

Peritoneal cells in mice: Quantitative and qualitative cell morphology

Effects of hydrocortisone on peritoneal free cells in mice.

Contact Info

Product

Resources

About