Age-Associated Mitochondrial Dysfunction in the Heart Accompanied by Constitutive NO-Synthases Uncoupling on the Background of Oxidative and Nitrosative Stress

Strutynska, Nataliya A.; Kotsiuruba, A V; Budko, Alina Yu.; Mys, Lidiya A.; Сагач, В. Ф.

doi:10.1615/intjphyspathophys.v8.i2.50

Cited by 5 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another important regulator is the gas transmitter H 2 S (hydrogen sulfi de), which is known for its vasodilator, antioxidant, antiapoptotic and pro-angiogenic properties, which provide cytoprotective and anti-infl ammatory effects [16,22]. A number of studies point out the unity of various ways of H 2 S synthesis: enzymatic and non-enzymatic (the transformation of thiols and thiol-containing compounds) [5,15,23]. The four most important enzymes involved in formation of hydrogen sulfi de are: cystathionine β-synthase -CBS, cystathionine γ-lyase -CSE, 3-mercaptopyruvate sulfurtransferase -3-MST, which cooperates with cysteine aminotrans- [17].…”

Section: Introductionmentioning

confidence: 99%

Atb 340 (A Modulator of Sulfite Oxidase Activity) Reduces Oxidative Stress During Hyperglycemia and in Stress Exposed Gastric Mucosa in Old Rats

Pavlovskiy

Zayachkivska

Lutsyk

et al. 2018

NTSN MS

View full text Add to dashboard Cite

Introduction. Sulfi te oxidase (SO) is one of the mitochondrial enzymes involved in the synthesis of H2S and multiple physiological processes, including oxidative stress. The dominant sulfi de oxidation products vary in a tissue-specifi c manner, and SO/oxidative stress which is involved in gastric mucosa (GM) injury is unclear. Our studies have shown potent cytoprotective and anti-infl ammatory effects of H2S-rich compounds, including hybrid nonsteroidal anti-infl ammatory drugs (H 2 S-NSAIDs) but their effects on age-related metabolic dysfunction in the gastric mucosa (GM) exposed to long-term postprandial hyperglycemia with high-fructose diet (HFD) is still unknown. Aim.To characterize the activity of sulfi te oxidase (SO) and the role of oxidative stress in the GM of older rats exposed to HFD and assess the therapeutic effect of the recently synthesized H 2 S donor, hybrid NSAID, H 2 S-acetylsalicylic acid (H 2 S-ASA, ATB 340) on the GM of adult vs old rats.Material and methods. Adult (n=24) and old (n=43) rats were divided into control groups with a standard diet (SD) and experimental groups with a 28-day high fructose level diet (HFD, V. Kozar, 2008) with and without acute stress induction (Takagi, 1964). During 19-28 day, HFD groups consumed per os: a) placebo (1.0 ml of saline per os); b) NaHS, 100 μmol/kg/day; c) placebo and stress induction; d) NaHS, 100 μmol/kg/day and stress induction; e) ASA, 10 mg/kg/ day and stress induction; f) conventional aspirin (ASA), 10 mg/kg/day and NaHS, 100 μmol/kg/ day and stress induction; g) ATB-340, 17.5 mg/kg/day and stress induction. The integrity of the GM was analyzed based on the histological severity index, which was calculated with account for morphological studies of GM samples. The indicator of oxidative stress, malondialdehyde (MDA) and the activity of SO in the GM were assessed using the standard biochemical methods. Results.We have observed a different SO expression in the GM of adult vs old rats with SD and HFD with and without WIS. The effect of HFD resulted in an increase in the SO activity in old rats by 15%, whereas in adults this value was 14%. MDA content changes were similar. In animals that received NaHS, the changes were similar, whereas the SO activity in old rats was 23% higher. Stress induction reduced the SO activity by 35% in old animals and by 22% in adults. The difference in the MDA content amounted to 41%. The effect of exogenous NaHS in old rats under stress was the highest when combined with ASA, while the MDA content was the highest when classical aspirin was used, which also coincided with similar changes in histological severity index. ОРИГІНАЛЬНІ ДОСЛІДЖЕННЯ: ФУНДАМЕНТАЛЬНІ НАУКИ ORIGINAL RESEARCH: BASIC SCIENCES Праці НТШ Медичні науки 2018, Том 54, № 2 NTSN MS 2018, Vol. 54, 2 Оригінальні дослідження: фундаментальні науки Original research: basic sciences Conclusion. Sulfi te oxidase is the essential contributor to age-related oxidative stress in the GM under research. The use of ATB-340 (H 2 S-aspirin) increases the H 2 S conten...

show abstract

Section: Introductionmentioning

confidence: 99%

Atb 340 (A Modulator of Sulfite Oxidase Activity) Reduces Oxidative Stress During Hyperglycemia and in Stress Exposed Gastric Mucosa in Old Rats

Pavlovskiy

Zayachkivska

Lutsyk

et al. 2018

NTSN MS

View full text Add to dashboard Cite

show abstract

Aminoguanidine Restores Endogenous Hydrogen Sulfide Synthesis, Constitutive Nitric Oxide Synthesis, Endothelium-Dependent Vasodilation and Inhibits Mitochondrial Permeability Transition Pore Opening in the Heart of Old Rats

Strutynska¹,

Korkach²,

Mys³

et al. 2019

Fiziol Zh

View full text Add to dashboard Cite

Effects of Glutathione on the Expression of Atp-Sensitive Potassium Channels, Mitochondrial Pores, and on Oxidative Stress in the Heart of Old Rats

Strutynska¹,

Goshovska²,

Korkach³

et al. 2020

Fiziol Zh

View full text Add to dashboard Cite

The effects of a single injection of the reduced form of glutathione on the expression of the KCNJ8 and KCNJ11 genes encoding Kir6.1 and Kir6.2 subunits of ATP-sensitive potassium (KATP) channels and on Ca2+-induced nonspecific mitochondrial permeability transition pores (mPTP) opening in the heart of old rats were studied. Changes in biochemical parameters characterizing the intensity of oxidative processes in organelles at the action of glutathione were also studied. The expression levels of Kir6.1 і Kir6.2 subunits KATP channels were determined using reverse transcription and quantitative PCR. It was shown that after the administration of glutathione to old rats, the expression of KATP channel subunits significantly increased, namely for Kir6.1 9.3 times, for Kir6.2 2.6 times. The use of glutathione in older animals inhibited mPTP opening: reduced the amplitude of spontaneous and Ca2+-induced swelling of mitochondria. An important consequence of the action of glutathione during aging was a decrease in the rate of generation of superoxide (.О2-) and hydroxyl (.ОН) radicals, as well as the content of hydrogen peroxide respectively in 1.8, 2.5 and 3.2 times compared with these figures in older animals without treatment. Glutathione also reduced lipid peroxidation, particularly pools of diene conjugates (2.5-fold) and malonic dialdehyde (1.8-fold). Thus, glutathione significantly increases the expression of KCNJ8 and KCNJ11 genes encoding Kir6.1 and Kir6.2 subunits of KATP channels in the heart of rats, regulates MP, preventing its opening, and reduces oxidative stress, indicating its important role in myocardial protection.

show abstract

Age-Associated Mitochondrial Dysfunction in the Heart Accompanied by Constitutive NO-Synthases Uncoupling on the Background of Oxidative and Nitrosative Stress

Cited by 5 publications

References 0 publications

Atb 340 (A Modulator of Sulfite Oxidase Activity) Reduces Oxidative Stress During Hyperglycemia and in Stress Exposed Gastric Mucosa in Old Rats

Atb 340 (A Modulator of Sulfite Oxidase Activity) Reduces Oxidative Stress During Hyperglycemia and in Stress Exposed Gastric Mucosa in Old Rats

Aminoguanidine Restores Endogenous Hydrogen Sulfide Synthesis, Constitutive Nitric Oxide Synthesis, Endothelium-Dependent Vasodilation and Inhibits Mitochondrial Permeability Transition Pore Opening in the Heart of Old Rats

Effects of Glutathione on the Expression of Atp-Sensitive Potassium Channels, Mitochondrial Pores, and on Oxidative Stress in the Heart of Old Rats

Contact Info

Product

Resources

About