Age-dependent changes and interrelations of number of cells and biochemical parameters (glucose, triglycerides, TBARS, calcium, phosphorus) in cultured human vein endothelial cells

Carrera-Rotllan, J; Estrada-Garcı́a, L

doi:10.1016/s0047-6374(98)00007-4

Cited by 11 publications

(3 citation statements)

References 22 publications

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“…The determination of lipid peroxidation was assessed by determining the thiobarbituric acid reactive species according to the method described by Ohkawa et al [29] and modified by Carrera-Rotllan and Estrada-Garcia [30]. After 72 hours of incubation, an aliquot of each treatment was mixed with a reaction medium containing 2-thiobarbituric acid (TBA) (0.8%) and incubated at 95°C for 1 hour.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Stability and In Vitro Security of Nanoemulsions Containing Eucalyptus globulus Oil

Godoi

Quatrin

Sagrillo

et al. 2017

BioMed Research International

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Essential oil of Eucalyptus globulus presents several pharmacological properties. However, their therapeutic efficacy may be affected by limitations due to several conditions, rendering it difficult to obtain stable and effective pharmaceutical formulations. The use of nanotechnology is an alternative to improve their characteristics aiming to ensure their stability and effectiveness. Furthermore, studies about the possible toxic effects of nanostructures are necessary to evaluate safety when the formulation comes into contact with human cells. Hence, in this paper, we evaluate for the first time the stability and in vitro cytogenotoxicity of nanoemulsions containing Eucalyptus globulus in peripheral blood mononuclear cells. As a result, the stability study found that the best condition for storage up to 90 days was refrigeration (4°C); it was the condition that best preserved the nanometric features. The content of the major compounds of oil was maintained after nanoencapsulation and preserved over time. In tests to evaluate the safety of this formulation, we can conclude that, at a low concentration (approximately 0.1%), Eucalyptus globulus nanoemulsion did not cause toxicity in peripheral blood mononuclear cells and also showed a protective effect in cells against possible damage when compared to oil in free form.

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Section: Methodsmentioning

confidence: 99%

Evaluation of Stability and In Vitro Security of Nanoemulsions Containing Eucalyptus globulus Oil

Godoi

Quatrin

Sagrillo

et al. 2017

BioMed Research International

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“…brain ( 70 ), kidney ( 71 , 72 ), liver ( 73 ) or heart ( 74 ). Increased formation of ROS ( 75 ), lipid peroxidation products and reactive aldehydic molecules (one of which would be malondialdehyde) has indeed been observed during aging ( 76–78 ). In addition, lipid peroxidation products have been suggested as one parameter in a possible set of clinical aging markers ( 79 ).…”

Section: Does Endogenous Formation Of Icls Increase With Age?mentioning

confidence: 99%

Contributions of DNA interstrand cross-links to aging of cells and organisms

Grillari‐Voglauer

Katinger

Voglauer

2007

Nucleic Acids Research

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Impaired DNA damage repair, especially deficient transcription-coupled nucleotide excision repair, leads to segmental progeroid syndromes in human patients as well as in rodent models. Furthermore, DNA double-strand break signalling has been pinpointed as a key inducer of cellular senescence. Several recent findings suggest that another DNA repair pathway, interstrand cross-link (ICL) repair, might also contribute to cell and organism aging. Therefore, we summarize and discuss here that (i) systemic administration of anti-cancer chemotherapeutics, in many cases DNA cross-linking drugs, induces premature progeroid frailty in long-term survivors; (ii) that ICL-inducing 8-methoxy-psoralen/UVA phototherapy leads to signs of premature skin aging as prominent long-term side effect and (iii) that mutated factors involved in ICL repair like ERCC1/XPF, the Fanconi anaemia proteins, WRN and SNEV lead to reduced replicative life span in vitro and segmental progeroid syndromes in vivo. However, since ICL-inducing drugs cause damage different from ICL and since all currently known ICL repair factors work in more than one pathway, further work will be needed to dissect the actual contribution of ICL damage to aging.

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“…Jedním z důvodů využívání anaerobní glykolýzy je nejspíše fakt, že ATP je tak získána podstatně rychleji než při aerobní glykolýze. 19 Jedna endotelová buňka v klidovém stavu spotřebuje za den asi 0,9 ng glukózy k výrobě 1× 10 11 mmol ATP, s poločasem ATP 8 s. 8,20 "Aerobní glykolýzu" můžeme jindy pozorovat u proliferujících buněk nebo u erytrocytů. Erytrocyty však na rozdíl od endotelových buněk nemají mitochondrie, a tak oxidativní metabolismus ani nemohou využít.…”

Section: Energetický Metabolismus Endotelové Buňky V Klidovém Stavuunclassified

(Energetic metabolism of endothelial cell)

Prokopcová¹

2019

Cor Vasa

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Endotelové buňky tvoří aktivní výstelku cévního systému, jsou metabolicky velmi aktivní a mají vysoké energetické požadavky. Hlavním zdrojem energie je glukóza spotřebovávaná anaerobní glykolýzou. Ostatní zdroje jsou využívány k tvorbě metabolických intermediátů potřebných v klidovém stavu k udržení buněčné struktury a k signalizaci, v aktivovaném stavu k cévní proliferaci. V klidovém stavu nejsou endotelové buňky příliš citlivé k hypoxii. Pokud jsou však endotelie současně vystaveny nedostatku glukózy a dodávka energetických substrátů a kyslíku není včas obnovena, dochází po čase k energetickému kolapsu a nekróze buňky.

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Age-dependent changes and interrelations of number of cells and biochemical parameters (glucose, triglycerides, TBARS, calcium, phosphorus) in cultured human vein endothelial cells

Cited by 11 publications

References 22 publications

Evaluation of Stability and In Vitro Security of Nanoemulsions Containing Eucalyptus globulus Oil

Evaluation of Stability and In Vitro Security of Nanoemulsions Containing Eucalyptus globulus Oil

Contributions of DNA interstrand cross-links to aging of cells and organisms

(Energetic metabolism of endothelial cell)

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