Age-Dependent Comparative Study of 4 Hz and 8 Hz EMF Exposure on Heart Muscle Tissue Hydration of Rats

Abstract

Previously we have shown that 4 Hz and 8 Hz EMF exposures have depressing effect on the thermodynamic activity of water, which decreases peroxide formation. It has also been shown that 4 Hz EMF-treated physiological solution modulates the growth and development of microbes and heart muscle contractility, but 8 Hz EMF has pronounced inhibitory effect on bacterial growth and development. Therefore, in order to elucidate the possible mechanism of 4 Hz and 8 Hz EMF effects on heart muscle function, in the present work the effects of 4 Hz and 8 Hz EMF exposures on heart muscle tissue hydration, the sensitivity of 4 Hz and 8 Hz EMF-induced tissue hydration to 10 −4 M ouabain (Na + /K + pump inhibition) and 10 −9 M ouabain (activation of intracellular signaling system) as well as the effects of 4 Hz and 8 Hz EMF exposures on the number of Na + /K + pump units in the membrane of both young and old rats have been studied. The obtained data allow us to suggest that 8 Hz EMF exposure has more pronounced age-dependent modulation effect on tissue hydration of heart muscle than 4 Hz EMF and this effect is sensitive to Na + /K + pump activity and intracellular signaling system.

The Quantum-Mechanical Sensitive Na/K Pump Is a Key Mechanism for the Metabolic Control of Neuronal Membrane Function

“…It is worth noting that in young animals the activation of both F Na/Ca and R Na/Ca exchanges stimulates water efflux from the cells, leading to the decrease of membrane permeability for Na, and Na efflux from the cells leads to the increase of Na gradient on membrane serving as energy sources for different secondary exchange systems. These data indicate that the Na/Ca exchange in both directions is a key protective cell mechanism, the dysfunction of which brings to cell pathology, including aging [65] [68].…”