Age-Dependent Expression of Apolipoprotein E in Mouse Cerebral Cortex

Singh, Surendra Pal; Thakur, M. K.

doi:10.1007/s12031-010-9412-3

Cited by 8 publications

(5 citation statements)

References 35 publications

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“…In this study the density levels of APOE-positive capillaries are slightly elevated in AD cases relative to those found in CG cases. A recent study in mice indicates that in the normal aging brain in mice APOE levels remain constant as a function of age [35]. There is no reported human study regarding this issue.…”

Section: Discussionmentioning

confidence: 98%

Correlation Analysis of Capillary APOE, VEGF and eNOS Expression in Alzheimer Brains

Provias

Jeynes²

2011

CAR

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Vasoactive regulatory and transport proteins can modify the transendothelial blood-brain barrier (BBB) transport of beta-amyloid. Dysfunction of one or more of these proteins is hypothesized to contribute to the pathogenesis of Alzheimer's disease (AD). In this study we investigated superior temporal and occipital cortical sections from ten AD and ten control group brains (CG). They were examined using immunohistochemical techniques staining for β(42) amyloid, APOE, VEGF and eNOS. The densities of senile plaques (SPs) and APOE, VEGF and eNOS positive capillaries in each region and in each AD and CG condition were compared using nonparametric statistical analysis. In the AD cases, there were significant negative correlations between APOE positive capillaries and β(42) amyloid SPs, and positive correlations between APOE positive capillaries and VEGF and eNOS positive capillaries. These results demonstrate the increased presence of APOE activity in AD brain capillaries, and that there is a positive correlation between the expression of APOE and each of VEGF and eNOS in the capillaries of AD brains. It is possible, therefore, that the down regulation of APOE in AD brains may contribute significantly to the pathogenesis of SP lesion development by modulating brain β-amyloid burden. The specific interrelationship between APOE, VEGF and eNOS activity at the BBB requires further investigation.

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Section: Discussionmentioning

confidence: 98%

Correlation Analysis of Capillary APOE, VEGF and eNOS Expression in Alzheimer Brains

Provias

Jeynes²

2011

CAR

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“…We reported that ApoE expression decreased from young to adult and then remained unchanged in old mouse cerebral cortex. Certainly, such expression profile suggested the involvement of other factors in maintaining ApoE level and dependent brain functions in old [ 28 ].…”

Section: Ad Associated Genesmentioning

confidence: 99%

Age-associated Cognitive Decline: Insights into Molecular Switches and Recovery Avenues

Konar¹,

Singh²,

Thakur³

2016

Aging and disease

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Age-associated cognitive decline is an inevitable phenomenon that predisposes individuals for neurological and psychiatric disorders eventually affecting the quality of life. Scientists have endeavored to identify the key molecular switches that drive cognitive decline with advancing age. These newly identified molecules are then targeted as recovery of cognitive aging and related disorders. Cognitive decline during aging is multi-factorial and amongst several factors influencing this trajectory, gene expression changes are pivotal. Identifying these genes would elucidate the neurobiological underpinnings as well as offer clues that make certain individuals resilient to withstand the inevitable age-related deteriorations. Our laboratory has focused on this aspect and investigated a wide spectrum of genes involved in crucial brain functions that attribute to senescence induced cognitive deficits. We have recently identified master switches in the epigenome regulating gene expression alteration during brain aging. Interestingly, these factors when manipulated by chemical or genetic strategies successfully reverse the age-related cognitive impairments. In the present article, we review findings from our laboratory and others combined with supporting literary evidences on molecular switches of brain aging and their potential as recovery targets.

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“…Also, some studies have shown that there is no age-related alteration in apoE protein level (Gee et al 2006;Ramaswamy et al 2005). Recently, our own study has shown that there is no age-related alteration in apoE level in adult and old male mice (Singh and Thakur 2010). So if there is no difference in apoE level due to age, it is likely that sex hormones have no effect on it.…”

Section: Western Blot Analysis Of Apoe In Gonadectomized and Hormone-mentioning

confidence: 91%

Gonadal Steroids do not Affect Apolipoprotein E Expression in Aging Mouse Cerebral Cortex

Singh

Thakur

2010

Cell Mol Neurobiol

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The allelic variant of apolipoprotein (Apo) E4 is a known risk factor for the development of most common late onset form of Alzheimer's disease (AD). As aging is associated with reduced circulating level of gonadal steroid hormones, hormone replacement therapies have been used for the possible treatment of AD. Both estrogen and testosterone have beneficial effects on brain due to interaction with apoE, but the underlying mechanism is still not clear. In this article, we report the effects of gonadectomy and hormone supplementation on apoE protein level in male and female mouse cerebral cortex during normal aging. We could not get any effect of gonadectomy and estradiol or testosterone treatment in adult and old mice of either sex. This suggests that during normal aging apoE protein level is not affected due to steroid hormone withdrawal or supplementation in the mouse cerebral cortex.

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Age-Dependent Expression of Apolipoprotein E in Mouse Cerebral Cortex

Cited by 8 publications

References 35 publications

Correlation Analysis of Capillary APOE, VEGF and eNOS Expression in Alzheimer Brains

Correlation Analysis of Capillary APOE, VEGF and eNOS Expression in Alzheimer Brains

Age-associated Cognitive Decline: Insights into Molecular Switches and Recovery Avenues

Gonadal Steroids do not Affect Apolipoprotein E Expression in Aging Mouse Cerebral Cortex

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