Age-dependent stability of nucleoli and global DNA methylation level in spermatocytes of the domestic horse (<i>Equus caballus</i>)

Andraszek, Katarzyna; Gryzińska, Magdalena; Danielewicz, A.; Batkowska, Justyna; Smalec, Elżbieta

doi:10.1139/cjas-2015-0076

Cited by 8 publications

(6 citation statements)

References 28 publications

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“…In the present study, an experimental technique of staining spermatozoa with silver nitrate colloid solution was used. As silver nitrate is an alkaline dye, it is mainly used to identify acidic chromatin proteins and the chromatin of nucleolus organizer regions in mitotic chromosomes [ 57 – 60 ] and nucleoli during meiosis [ 61 , 62 ] The basic methodology has been modified in our Department (Department of Animal Genetics and Horse Breeding, University of Natural Sciences and Humanities in Siedlce) and successfully used to identify morphological details of the spermatozoa either from fresh, frozen or fixed semen sample of mammals [ 42 – 44 , 63 , 64 ], birds [ 47 ] and insects. [ 36 ]…”

Section: Introductionmentioning

confidence: 99%

The effect of the staining technique on morphological and morphometric parameters of boar sperm

et al. 2019

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Sperm morphology and morphometry are important parameters in predicting fertility. Sperm are considered to be normal if the shape and size of the head, midpiece and tail fall within the classification for a given species. It is important to select the appropriate technique for staining the semen of a given species, because, as many authors have pointed out, some methods work well for one species but are not suitable for analysing another. The aim of the study was to assess the morphometric parameters of boar sperm following the use of different staining techniques and to verify the hypothesis that the staining technique affects the morphometric parameters of sperm. The staining method was found to significantly affect the dimensions of the boar sperm head. The semen stained by the SpermBlue technique had the closest morphometric sperm head parameters to those of the unstained sperm, so this technique, rather than the routinely used eosin and gentian complex, should be the leading technique in the evaluation of boar sperm morphometry. Silver nitrate staining reveals the structure of the sperm in the most detail; this method can be considered universal, and can be used independently or to supplement routine diagnostics. As the staining technique should interfere as little as possible with the structure of the sperm, while revealing its morphology in as much detail as possible, it is crucial to establish the natural dimensions of the unstained sperm head before determining the optimal technique and its reference values. The recommended or most commonly-used techniques are not always the best options for the staining and analysis of sperm of a given species.

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Section: Introductionmentioning

confidence: 99%

The effect of the staining technique on morphological and morphometric parameters of boar sperm

et al. 2019

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“…According to published data, the presence of a tetraploid karyotype leads to the cessation of division, acceleration of cellular aging, and apoptosis (1). Changes in the amount of genetic material during cultivation can lead to abnormal functioning of the cell genome and negative effects in the cell, including oncogenic transformation (5).…”

Section: Discussionmentioning

confidence: 99%

Immunophenotypic characterisation and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture

Mazurkevych

Malyuk

Bezdieniezhnykh

et al. 2016

Journal of Veterinary Research

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Introduction: The objective of the study was immunophenotypic and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture. Material and Methods: The mesenchymal stem cells were obtained from equine bone marrow of three horses and from foal umbilical cords of six foals. The cells were cultured in CO2 incubators by standard procedure. Quantitative abnormalities of chromosomes, i.e. aneuploidy and polyploidy, and structural aberrations, i.e. breaks in chromosomes and chromatid, were taken into account during the study. Results: The results of cytogenetic analysis of equine bone marrow mesenchymal stem cells at the third and fourth passages indicated that the level of karyotype variability of these cells corresponded to the spontaneous level of karyotype variability typical of the peripheral blood lymphocytes of this species. Equine bone marrow contained several clones of stem cells that differed in the expression of specific nuclear markers characteristic of proliferating cells. Conclusion: Mesenchymal stem cells from foal umbilical cords during in vitro cultivation are characterised by quantitative abnormalities of the chromosomal apparatus.

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“…In addition to the main livestock species discussed above, some epigenetics-related data have been reported in other livestock species. For instance, DNA methylation was predicted to be an age-dependent process in domestic horses ( Andraszek et al, 2016 ). Different from the generally methylated mammalian genomes, honeybees have unique methylation patterns that concentrate in gene bodies and are associated with gene expression ( Wedd and Maleszka, 2016 ; Harris et al, 2019 ).…”

Section: Evidence Of the Impacts Of Epigenetic Processes On Livestockmentioning

confidence: 99%

Impacts of Epigenetic Processes on the Health and Productivity of Livestock

Wang

Ibeagha‐Awemu

2021

Front. Genet.

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The dynamic changes in the epigenome resulting from the intricate interactions of genetic and environmental factors play crucial roles in individual growth and development. Numerous studies in plants, rodents, and humans have provided evidence of the regulatory roles of epigenetic processes in health and disease. There is increasing pressure to increase livestock production in light of increasing food needs of an expanding human population and environment challenges, but there is limited related epigenetic data on livestock to complement genomic information and support advances in improvement breeding and health management. This review examines the recent discoveries on epigenetic processes due to DNA methylation, histone modification, and chromatin remodeling and their impacts on health and production traits in farm animals, including bovine, swine, sheep, goat, and poultry species. Most of the reports focused on epigenome profiling at the genome-wide or specific genic regions in response to developmental processes, environmental stressors, nutrition, and disease pathogens. The bulk of available data mainly characterized the epigenetic markers in tissues/organs or in relation to traits and detection of epigenetic regulatory mechanisms underlying livestock phenotype diversity. However, available data is inadequate to support gainful exploitation of epigenetic processes for improved animal health and productivity management. Increased research effort, which is vital to elucidate how epigenetic mechanisms affect the health and productivity of livestock, is currently limited due to several factors including lack of adequate analytical tools. In this review, we (1) summarize available evidence of the impacts of epigenetic processes on livestock production and health traits, (2) discuss the application of epigenetics data in livestock production, and (3) present gaps in livestock epigenetics research. Knowledge of the epigenetic factors influencing livestock health and productivity is vital for the management and improvement of livestock productivity.

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Age-dependent stability of nucleoli and global DNA methylation level in spermatocytes of the domestic horse (Equus caballus)

Cited by 8 publications

References 28 publications

The effect of the staining technique on morphological and morphometric parameters of boar sperm

The effect of the staining technique on morphological and morphometric parameters of boar sperm

Immunophenotypic characterisation and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture

Impacts of Epigenetic Processes on the Health and Productivity of Livestock

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