Age-related and clinicopathological features of colorectal cancer associated with K-ras gene status

Belyaeva, A.; Yanus, Grigoriy A.; Суспицын, Е. Н.; Zaitseva, Olga A.; Yatsuk, Olga S.; Moiseenko, A.; Gulyaev, A. V.; Imyanitov, Evgeny N.

doi:10.1134/s2079057012040030

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…Genic mutation of K-ras could be concerned as early molecular events (Onozato et al, 2011;Beliaeva et al, 2012); However, koinonia of HPV infection and gene K-ras might play a certain part in formation of rectal cancer. However, is HPV infection one of Initiating factors or accompanied infection after rectal cancer formation?…”

Section: Discussionmentioning

confidence: 99%

Significance of HPV Infection and Genic Mutation of APC and K-ras in Patients with Rectal Cancer

Sun

Wang²,

Zhao³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Background: Significance of HPV infection and genic mutation of APC and K-ras in rectal cancer has been investigated but not clarified. The objective of our study was to investigate these parameters in patients with rectal cancer to analyze correlations with biological behaviour, to determine relationships among the three, and also to demonstrate survival prognosis effects. Methods: From December 2007 to September 2008, 75 rectal cancer cases confirmed by histopathology in the Tumor Hospital of Xinjiang Medical University were enrolled. The control group consisted of normal rectal mucous membrane taken simultaneously, a least 10 cm distant from the carcinoma fringe. HPV DNA, the MCR of APC and exon-1 of K-ras were detected by PCR and PCR-SSCP. All results were analyzed in relation to clinical pathological material, using chi-square and correlation analysis via SPSS.13 and Fisher's Exact Probability via STATA. 9.0. All 75 patients were followed up for survival analysis using Kaplan-Meier and Log-rank tests. Results: 55 out of 75 cases demonstrated gene HPV L1 while it was notdetected in normal rectal mucosa tissue. HPV infection was correlated with age and lymphatic metastasis (P<0.05) but not other characteristics, such as ethnicity, tumor size, histological type, tumor type, Duke's stage and infiltration depth. Some 43 cases exhibited APC genic mutation (57.3%) and 34 K-ras genic mutation (45.3%). APC genic mutation was correlated with gender( P<0.05), but not age, histological type, infiltration depth, lymphatic metastasis and Duke's stage. In 55 cases of rectal cancer with HPV infection, there were 31 cases with genic mutation of APC (56.4%) and 24 with genic mutation of K-ras (43.6%). For the 20 cases of rectal cancer with non-HPV infection, the figures were 12 cases (60%) and 10 (50.0%), respectively, with no significant relation. Survival analysis showed no statistical significance for K-ras genic mutation, APC genic mutation or HPV infection (P>0.05). However, the survival time of the patients with HPV infection was a little shorter than in cases without HPV infection. Conclusions: Our results suggest that HPV infection might be an important factor to bring about malignant phenotype of rectal cancer and influence prognosis. Genic mutation of APC and K-ras might be common early molecular events of rectal cancer, but without prognostic effects on medium-term or early stage patients with rectal cancer.

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Section: Discussionmentioning

confidence: 99%

Significance of HPV Infection and Genic Mutation of APC and K-ras in Patients with Rectal Cancer

Sun

Wang²,

Zhao³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Clinical Characteristics of Colorectal Cancer in Patients With Egfr-Signaling Pathway Gene Mutations

Богомолова

Антонеева

Долгова

2019

Ulyanovsk Medico-Biological Journal

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KRAS, NRAS, BRAF mutations are associated with an unfavorable prognosis for colorectal cancer (CRC). At the same time, there is no single point of view on disease development during adjuvant chemotherapy (ACT). Objective. The authors aimed at studying KRAS, NRAS and BRAF mutations in the tumor and their influence on the clinical characteristics of colorectal cancer development. Materials and Methods. Paraffin blocks of the primary CRC tumor (n=37) were used as the material for the study. Using genomic DNA, isolated from the primary tumor, real-time PCR was used to determine the most common mutations in CRC: KRAS gene (exon 2, codon region 12–13), NRAS gene (exon 3, codon region 61), B6F V600E gene. Results. The results of genotyping of DNA samples isolated from the primary CRC tumor paraffin blocks showed that BRAF gene mutations were detected in 8.2 % of cases, NRAS gene mutations were detected in 5.4 % of cases, and KRAS gene mutations were detected in 37.8 % of cases. The authors didn’t reveal any dependencies of the mutation distribution on patients’ gender and age. The examined mutations were more common in adenocarcinomas of high and moderate degrees of differentiation. The relapse-free period after ACT in patients with identified KRAS, NRAS, BRAF gene mutations is significantly less than in those without mutations. Conclusion. The findings suggest that EGFR signaling pathway mutations (KRAS, NRAS and BRAF) increase the risk of disease recurrence and are an unfavorable prognostic factor. Keywords: colorectal cancer, NRAS, KRAS, BRAF mutations, adjuvant chemotherapy.

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Age-related and clinicopathological features of colorectal cancer associated with K-ras gene status

Cited by 2 publications

References 15 publications

Significance of HPV Infection and Genic Mutation of APC and K-ras in Patients with Rectal Cancer

Significance of HPV Infection and Genic Mutation of APC and K-ras in Patients with Rectal Cancer

Clinical Characteristics of Colorectal Cancer in Patients With Egfr-Signaling Pathway Gene Mutations

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