Age-Related Binding of Proximal Region of ApoE Promoter to Nuclear Proteins of Mouse Cerebral Cortex

Abstract

Mouse ApolipoproteinE (ApoE) gene has maximum promoter activity in the proximal region (-212 to +54) which includes different regulatory elements. These elements bind to specific protein factors and influence the expression of genes which are involved in key brain functions that decline with age. As there is no information on the binding of apoE promoter to nuclear proteins as a function of age, we have analyzed the binding of USF, AP1 and one negative element sequence present in ApoE proximal promoter to nuclear proteins of the cerebral cortex of mice of different ages. The findings show the formation of one complex with USF and two complexes with AP1 and negative element. The intensity of these complexes varies with age, indicating differential binding of protein factors to specific elements of apoE promoter, which reflect age-related regulation of apoE -mediated brain functions.

Methyl‐donor deficiency in adolescence affects memory and epigenetic status in the mouse hippocampus

“…We tried speculative transcription factor binding in this region using TFSERCH (http://www.cbrc.jp/research/db/TFSEARCH.html), and found one candidate gene, upstream stimulatory factor 1 (USF1) (data not shown). USF has been shown to be associated with the expression of ApoE declined with age in cerebral cortex (Singh & Thakur ). In addition, age‐related difference of number of GluR1‐positive neurons in frontal cortex has been reported (Kim et al ).…”