Age-Related Change in Rat Testicular ATPase Activities in Response to HCH Treatment

Abstract

1 HCH (Y-hexachlorocyclohexane), an organochlorine insecticide is used widely for agricultural and hygienic purposes in most of the developing countries including India. Like other organochlorine, HCH can also enter into the body by various means such as through food chain, through inhalation and through dermal contact. It has been reported that HCH preferably accumulates in adipose tissues and in membrane lipid layer (Zhu et al. 1986; Lopez-Aparicio et al.1 988). Besides exhibiting neurological (Woolley et al. 1985;Tusell et al. 1987) and hepatological (Junqueira et al.1988) toxicities, HCH is also reported to produce adverse effect on male reproductive systems of rats. HCH-induced toxicity in male reproductive system includes degeneration of seminiferous tubules (Dikshith et al.1978 ; Srinivasan et a1.1988) and inhibition of stimulatory activity of adrenergic agonist upon CAMP accumulation in prostatic epithelial cells (Carrero et al. 1990). Mechanism of action of HCH on male reproductive system particularly in the testis is poorly understood. Mitochondrial fraction from rat testis has been shown to metaboiize ATP at a faster rate than that from liver or kidney (Hollinger 1971). Although testis from immature and mature rats catabolized ATP at a very high rate (Hollinger 1971), by means of Ca +2 and Mg +2 ATPase, there is a marked change in the enzyme activity during maturation (DelhumeauOngay et al.1973). It is understood that pesticide-induced testicular damage is more pronounced in immature rats in comparison to adult rats (Sjoberg et al.1985 Jinna et al.1989. Keeping this in view, an effort has been made in the present study to examine the effect of chronic exposure of HCH on testicular Ca +2 and Mg +2 -ATPase and Mg +2 ATPase in immature (30 days old) and adult (90 days old) rats since no information on this subject is yet available. MATERIALS AND METHODSMale wistar rats of 30 day old (weighing 60-80 g.) and 90 day old (weighing 300-350 g ) were purchased from National Institute of Nutrition, Hyderabad, India. They were acclimated to laboratory conditions for about 10 days before any experimentation. Rats were maintained with artificial illumination for 12 h followed by a dark period of 12 h. They were fed gram (Cicer arietinum) and freshly prepared diet containing flour and vitaminized powered milk. Tap water was supplied ad libitum.Rats of each age group were divided into three groups containing 5 animals per group. The second and third group were fed 10 mg and 20 mg HCH per kg. body weight respectively by oral intubation for 7, 15 and 30 days. The first group which served as control, was fed only olive oil in the similar manner. The technical grade HCH, obtained

Possible activation of NRF2 by Vitamin E/Curcumin against altered thyroid hormone induced oxidative stress via NFĸB/AKT/mTOR/KEAP1 signalling in rat heart

“…The activity of Ca 2+ ATPase was assayed in the membrane fraction (10,000 × g pellet of the crude homogenate dissolved in the homogenizing buffer) according to the method of Roy and Chainy 20 . The samples (40 µg protein) were incubated in standard incubation medium consisting of 50 mM Tris-HCl, pH 7.4, 0.5 mM EGTA and 5 mM Ca 2+ .…”

An Environmentally Relevant Organochlorine Mixture Impairs Sperm Function and Embryo Development in the Porcine Model1

“…It has been shown that methoxychlor and dichlorodiphenyltrichloroethane (DDT), but not lindane, induce sublethal changes in transmembrane potential, ROS production, and ATP-induced intracellular Ca 2ϩ homeostasis in bovine oviductal cells [43]. However, lindane and dieldrin stimulate ROS production in neutrophils by activating phospholipase A 2 [54], and lindane alters rat testicular Ca 2ϩ -and Mg 2ϩ -ATPase activities [55]. Perturbations in intracellular Ca 2ϩ homeostasis and in Ca 2ϩ uptake by mitochondria was also observed in rat cerebellar granule cells following PCB exposure [42,56].…”

Changes in rat testicular antioxidant defence profile as a function of age and its impairment by hexachlorocyclohexane during critical stages of maturation

“…Animals kom each group were sacrificed on the 46th day (onset of reproductive capacity) of age by decapitation (n = 5 for each parameter studied) and the testes were quickly dissected out, cleaned in normal saline (0.9%, w/v) solution and processed immediately for estimation of various oxidative stress parameters (Samanta & Chainy, 1997). Testicular mitochondria1 Ca2+-Mgz+-ATPase activity was estimated as described earlier (Roy & Chainy, 1996).…”

“…Earlier it was reported that hexachlorocyclohexane (HCH), an organochlorine pesticide, induces oxidative stress in the testis and the magnitude of induction is higher in the case of immature rats than the mature ones (Samanta & Chainy, 1997). Similarly, alteration in the mitochondrial ATPases of the rat testis to HCH is more pronounced in immature rats than their mature counterparts (Roy & Chainy, 1996). In the present study, in order to understand the impact of HCH exposure during critical stages of testicular development, rats were exposed to HCH during critical stages of sexual maturation (from 6 to 30 days of age) and changes in oxidative stress parameters and mitochondrial Ca2 +-Mg*+-ATPases in the testis were recorded on the 46th day (onset of reproductive capacity) of age.…”