Age-related change in serotonin-mediated prejunctional inhibition of rat vas deferens

Moritoki, Hideki; Iwamoto, Takeshi; Kanaya, Jun; Ishida, Yoshiteru; Fukuda, Hiroshi

doi:10.1016/0014-2999(86)90006-3

Cited by 10 publications

(2 citation statements)

References 13 publications

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“…Ageing has been shown to increase the risk of ureteral calculus development (Hess, 2003) and this could be related to changes in ureteral function, although the effects of age on ureteral responses is unknown. Age-related changes in 5-HT contractile mechanism have been observed in other systems such as the gastrointestinal system (Keating, Nocchi, Yu, Donovan, & Grundy, 2015) and the inhibitory effect of 5-HT2 receptor stimulation in the rat vas deferens has been found to decrease with increasing age (Moritoki, Iwamoto, Kanaya, Ishida, & Fukuda, 1986). The aim of this study was to identify the receptor subtype mediating ureteral response to 5-HT and to determine whether age has any effect on these responses.…”

Section: Introductionmentioning

confidence: 96%

5-HT2A receptor is the predominant receptor mediating contraction of the isolated porcine distal ureter to 5-HT in young and old animals

Lim

Chess-Williams

Sellers

2018

European Journal of Pharmacology

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Isolated ureteral strips develop spontaneous phasic contractile activity which is enhanced by 5-hydroytryptamine (5-HT). The aim of this study was to identify the receptor subtype mediating these responses and to determine whether responses to 5-HT change with age. The frequency of contractions and the overall contractile activity (measured as the area under the curve, AUC) were recorded in strips of porcine distal ureter isolated from young (3 months) and old (2 years) pigs. Responses to 5-HT were examined in the absence and presence of selective 5-HT receptor subtype antagonists. Tissues from the younger animals elicited larger contractile responses to 5-HT (5885 ± 335g s) than tissues from the older animals (2787 ± 317g s, P < 0.001). The 5-HT receptor antagonist ketanserin (10-100nM) elicited rightward shifts of 5-HT concentration-response curves, antagonising AUC and frequency responses with high affinity in tissues from both age groups (pK values 8.4-8.8). The slopes of the corresponding Schild plots were not significantly different from unity, suggesting a competitive antagonism at a single receptor, except for frequency responses to 5-HT in the older animals. Antagonists selective for other 5-HT receptor subtypes: methiothepin (non-selective), RS-10221 (5-HT), ondansetron (5-HT), GR-113808 (5-HT), SB699551 (5-HT), SB39988 (5-HT), SB269970 (5-HT)) had no effect on 5-HT-induced responses. The results suggest that the 5-HT receptor subtype is the predominant receptor mediating 5-HT responses in ureteral tissues, being the sole mediator of responses in tissues from young animals, but with another receptor subtype also playing a minor role in the older animals.

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Section: Introductionmentioning

confidence: 96%

5-HT2A receptor is the predominant receptor mediating contraction of the isolated porcine distal ureter to 5-HT in young and old animals

Lim

Chess-Williams

Sellers

2018

European Journal of Pharmacology

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“…Studies on conduit arteries have demonstrated changes in endothelium-dependent control with postnatal growth, although there are inconsistent findings. For example, endothelium-dependent dilator responses to histamine are similar in large mesenteric arteries from 2-and 8-week-old rats, but then decline to smaller responses in arteries from 13-week-old rats (Moritoki et al, 1986). In contrast, the endothelium-dependent relaxation of porcine pulmonary arteries to ACh, BK and the calcium ionophore A23187…”

Section: B Conduit Arteries I Endothelium-dependent Controlmentioning

confidence: 97%

Factors regulating arteriolar tone during microvascular growth

Samora¹

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Growth of the arteriolar network is accompanied by progressive changes in pressure and flow, and in the metabolic environment to which the arterioles are exposed. This dissertation was carried out to investigate the extent to which mechanisms involved in local blood flow regulation may also change during this growth. We isolated gracilis muscle arterioles from weanling (age 25-26 days) and juvenile (44-66 days) rats, and studied the responsiveness of these vessels to both myogenic and endothelium-dependent stimuli. Overall arteriolar responses to the endothelium-dependent agonists acetylcholine (ACh), A23187, VEGF and simvastatin were not different between age groups. Responses of juvenile arterioles to ACh and simvastatin were significantly reduced by inhibition of nitric oxide synthase or cyclooxygenase, by the hydrogen peroxide scavenger catalase, and by potassium channel inhibition. Responses of weanling arterioles to ACh and simvastatin were unaffected by these treatments, but significantly reduced by heme oxygenase inhibition. Arteriolar growth during juvenile maturation is accompanied by an increase in myogenic responsiveness, possibly because endotheliumderived PGH 2 or TXA 2 assumes a role in reinforcing myogenic activity over this period. In conclusion, mediators of endothelium-dependent dilation change during growth, with carbon monoxide contributing largely to these responses in young animals, and a combination of nitric oxide, prostanoids and hydrogen peroxide contributing to these responses in older animals. These data suggest that age-dependent differences in the control of vascular tone exist and may have significant implications for the regulation of tissue perfusion. DEDICATION I would like to dedicate this work to my parents, Pamela and Patrick Balch, who have offered unwavering encouragement and support, instilled in me the self-confidence to fulfill my dreams, and who have stood by my side through every decision, accomplishment, and failure. I love you both so much. Thanks for being the amazing parents you are. iii Jefferson C. Frisbee for their support and faith in my abilities. They have been a great team-two completely different personalities and research interests, but both providing extremely valuable insight and support. Wherever they may have differed in their overall objectives for me, they were always cooperative and accommodating, and had my best interests in mind. Dr. Frisbee-you have an intense ambition and motivation that inspire and amaze me. Dr. Boegehold-I greatly appreciate all of your efforts during these last few months not only working with me to understand some difficult data and helping to get the last few manuscripts into shape, but also in assisting me with the completion of this work. As an aside, I hope I may someday become as skilled in the art of diplomacy as you! Thank you both for taking me under your wings. I have learned a great deal under your mentorship.

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Pre-synaptic 5-HT Receptors Mediating Inhibition of Transmitter Release from Peripheral Cholinergic and Noradrenergic Nerves

Clarke¹,

Bond

Charlton

et al. 1989

Serotonin

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Age-related change in serotonin-mediated prejunctional inhibition of rat vas deferens

Cited by 10 publications

References 13 publications

5-HT2A receptor is the predominant receptor mediating contraction of the isolated porcine distal ureter to 5-HT in young and old animals

5-HT2A receptor is the predominant receptor mediating contraction of the isolated porcine distal ureter to 5-HT in young and old animals

Factors regulating arteriolar tone during microvascular growth

Pre-synaptic 5-HT Receptors Mediating Inhibition of Transmitter Release from Peripheral Cholinergic and Noradrenergic Nerves

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