Age-Related Changes in Microglia of the Rat Spinal Cord

Kolos, E. A.; Коржевский, Д. Э.

doi:10.1134/s0022093022040172

Cited by 3 publications

(1 citation statement)

References 47 publications

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“…There is some evidence demonstrating that the aged spinal cord exhibits axonal loss and demyelination, similar to the aging brain (25)(26)(27). This process is likely associated with significantly elevated microglia density, particularly microglia with morphological changes characteristic of a reactive phenotype, in the aged WM (28). One study found a number of cystic regions within the WM associated with elevated staining for glial fibrillary acidic protein (GFAP) and an increased prevalence of Marchi positive bodies in aged rats (29).…”

Section: Discussionmentioning

confidence: 99%

Contrast-enhanced ultrasound imaging detects anatomical and functional changes in rat cervical spine microvasculature with normal aging

Harmon,

Chandran,

Chandrasekaran

et al. 2024

Preprint

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Normal aging is associated with significant deleterious cerebrovascular changes; these have been implicated in disease pathogenesis and increased susceptibility to ischemic injury. While these changes are well documented in the brain, few studies have been conducted in the spinal cord. Here, we utilize specialized contrast-enhanced ultrasound (CEUS) imaging to investigate age-related changes in cervical spinal vascular anatomy and hemodynamics in male Fisher 344 rats, a common strain in aging research. Aged rats (24-26 mo., N=6) exhibited significant tortuosity in the anterior spinal artery and elevated vascular resistance compared to adults (4-6 mo., N=6; tortuosity index 2.20±0.15 vs 4.74±0.45, p<0.05). Baseline blood volume was lower in both larger vessels and the microcirculation in the aged cohort, specifically in white matter (4.44e14±1.37e13 vs 3.66e14±2.64e13 CEUS bolus AUC, p<0.05). To elucidate functional differences, animals were exposed to a hypoxia challenge; whereas adult rats exhibited significant functional hyperemia in both gray and white matter (GM: 1.13±0.10-fold change from normoxia, p<0.05; WM: 1.16±0.13, p<0.05), aged rats showed no response. Immunohistochemistry revealed reduced pericyte coverage and activated microglia behavior in aged rats, which may partially explain the lack of vascular response. This study provides the first in vivo description of age-related hemodynamic differences in the cervical spinal cord.

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Section: Discussionmentioning

confidence: 99%

Contrast-enhanced ultrasound imaging detects anatomical and functional changes in rat cervical spine microvasculature with normal aging

Harmon,

Chandran,

Chandrasekaran

et al. 2024

Preprint

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Reactive Changes in Rat Spinal Cord Microgliocytes under Acute Systemic Inflammation

Kolos,

Korzhevskii

2023

J Evol Biochem Phys

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Reactive Changes of Rat Spinal Cord Microgliocytes after Acute Systemic Inflammation

Kolos,

Korzhevskii

2023

Rossijskij fiziologičeskij žurnal im. I.M. Sečenova

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It is widely known that neuroinflammation is a key factor in the development of many neurological pathologies and neurodegenerative diseases. The dynamics of development and duration of neuroinflammatory responses are critical aspects in understanding the patterns of physiological, biochemical and behavioral consequences. The most common object of study is neuroinflammation that develops after experimental systemic inflammation. The effect of acute systemic inflammation on brain microgliocytes has been studied extensively, while spinal cord microglia have been studied less frequently. The purpose of this study was to assess the topographic and temporal features of morphofunctional changes in rat spinal cord microglial cells after experimental LPS-induced systemic inflammation. It has been established that in the early stages of neuroinflammation (24 hours after LPS administration), microgliocytes are activated in the ventral white and ventral gray matter of the spinal cord. At the same time, microgliocytes of the dorsal part of the spinal cord do not show morphological attribute of activation. An increase in the population density of microgliocytes in the ventral funiculus of the spinal cord was noted. Accumulations (aggregates) of reactive microgliocytes were also found in this area.

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Age-Related Changes in Microglia of the Rat Spinal Cord

Cited by 3 publications

References 47 publications

Contrast-enhanced ultrasound imaging detects anatomical and functional changes in rat cervical spine microvasculature with normal aging

Contrast-enhanced ultrasound imaging detects anatomical and functional changes in rat cervical spine microvasculature with normal aging

Reactive Changes in Rat Spinal Cord Microgliocytes under Acute Systemic Inflammation

Reactive Changes of Rat Spinal Cord Microgliocytes after Acute Systemic Inflammation

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