Age-related changes in protein metabolism of beech (Fagus sylvatica L.) seeds during alleviation of dormancy and in the early stage of germination

Ratajczak, Ewelina; Kalemba, Ewa Marzena; Pukacka, Stanisława

doi:10.1016/j.plaphy.2015.06.003

Cited by 6 publications

(5 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Last but not least, proteomics has been employed to analyze the proteome of seeds and changes associated to seed maturation and germination in an attempt to characterize and differentiate, at the molecular level, orthodox and non-orthodox species and zygotic and somatic embryos ([18,19,86,87,88,89,90,91,92,93]; this study is of great importance for propagation and seed conservation programs.…”

Section: How Quercus Ilex Is Seen By Proteomicsmentioning

confidence: 99%

Proteomics, Holm Oak (Quercus ilex L.) and Other Recalcitrant and Orphan Forest Tree Species: How do They See Each Other?

Rey

Castillejo

Sánchez-Lucas

et al. 2019

IJMS

View full text Add to dashboard Cite

Proteomics has had a big impact on plant biology, considered as a valuable tool for several forest species, such as Quercus, Pines, Poplars, and Eucalyptus. This review assesses the potential and limitations of the proteomics approaches and is focused on Quercus ilex as a model species and other forest tree species. Proteomics has been used with Q. ilex since 2003 with the main aim of examining natural variability, developmental processes, and responses to biotic and abiotic stresses as in other species of the genus Quercus or Pinus. As with the progress in techniques in proteomics in other plant species, the research in Q. ilex moved from 2-DE based strategy to the latest gel-free shotgun workflows. Experimental design, protein extraction, mass spectrometric analysis, confidence levels of qualitative and quantitative proteomics data, and their interpretation are a true challenge with relation to forest tree species due to their extreme orphan and recalcitrant (non-orthodox) nature. Implementing a systems biology approach, it is time to validate proteomics data using complementary techniques and integrate it with the -omics and classical approaches. The full potential of the protein field in plant research is quite far from being entirely exploited. However, despite the methodological limitations present in proteomics, there is no doubt that this discipline has contributed to deeper knowledge of plant biology and, currently, is increasingly employed for translational purposes.

show abstract

Section: How Quercus Ilex Is Seen By Proteomicsmentioning

confidence: 99%

Proteomics, Holm Oak (Quercus ilex L.) and Other Recalcitrant and Orphan Forest Tree Species: How do They See Each Other?

Rey

Castillejo

Sánchez-Lucas

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…Based on our previous studies of beech seed aging, the QC, an initial area of the apical meristem, was found to be a region accumulating ROS during seed storage and aging [14]. Moreover, higher ROS levels were associated with lower seed germinability [14]. Thus, we hypothesize that dehydration-initiated excessively low O [67].…”

Section: Discussionmentioning

confidence: 84%

“…H 2 O 2 diffuses across cell membranes through the aquaporin channels [66] and spreads freely through disintegrated membranes in damaged tissue. Based on our previous studies of beech seed aging, the QC, an initial area of the apical meristem, was found to be a region accumulating ROS during seed storage and aging [14]. Moreover, higher ROS levels were associated with lower seed germinability [14].…”

Section: Discussionmentioning

confidence: 97%

“…A dehydration-induced similar phenomenon was observed in beech elongating embryonic axes. The accumulation of ROS above a specific threshold level switches the ROS function from a useful signaling molecule to a deleterious one [14,30,63]. H 2 O 2 overproduction detected in radicles of elongating embryonic axes at the II-V early seedling establishment stages is probably linked to oxidative stress that occurs in dehydration-sensitive seed tissues [35] because enhanced H 2 O 2 production is not the element of the radicle elongation program [63].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the accumulation of storage materials has important protective benefits during dehydration and desiccation [12]. Actually, the germination capacity of beech seeds is related to lipid composition [13] and protein metabolism [14]. The above data encouraged us to investigate the level of lipids in beech during dormancy breaking and the three germination phases when growth processes consume this energy source, desiccation tolerance is gradually lost and dehydration-sensitive seedlings are produced [10,15] to examine whether the germination program is executed properly.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dehydration Sensitivity at the Early Seedling Establishment Stages of the European Beech (Fagus sylvatica L.)

Kalemba

Bagniewska‐Zadworna

Suszka

et al. 2019

Forests

View full text Add to dashboard Cite

Shortage of water is a limiting factor for the growth and development of plants, particularly at early developmental stages. We focused on the European beech (Fagus sylvatica L.), which produces seeds and further seedlings in large intervals of up to ten years. To explore the beech seedling establishment process, six stages referring to embryo expansion were studied to determine sensitivity to dehydration. The characterization of the response of elongating embryonic axes and cotyledons included a viability test before and after dehydration and measurement of the amounts of electrolyte leakage, concentration, and arrangement of storage materials, changes in chaperone proteins related to water deficit, and accumulation of hydrogen peroxide and superoxide anion radicals. Elongating embryonic axes and cotyledons differed in water content, dehydration rates, membrane permeability before and after dehydration, protein, and lipid decomposition pattern, and amount of 44-kDa dehydrin and 22-kDa small heat shock protein (sHSP). Protruding embryonic axes were more sensitive to dehydration than cotyledons, although dehydration caused transient reinduction of three dehydrin-like proteins and sHSP synthesis, which accompany desiccation tolerance. Extended deterioration, including overproduction of hydrogen peroxide and depletion of superoxide anion radicals, was reported in dehydrated embryonic axes longer than 10 mm characterized by highly elevated cellular leakage. The apical part elongating embryonic axes consisting of the radicles was the most sensitive part of the seed to dehydration, and the root apical meristem area was the first to become inviable. The effects of severe dehydration involving ROS imbalance and reduced viability in beech seedlings with embryonic axes longer than 10 mm might help to explain the difficulties in beech seedling establishment observed in drought-affected environments. The conversion of environmental drought into climate-originated oxidative stress affecting beech seedling performance is discussed in this report.

show abstract

Effects of storage on seed germination and viability for three native tree species of Ecuador

Palomeque

Uyaguari

Marín

et al. 2020

Trees

View full text Add to dashboard Cite

Age-related changes in protein metabolism of beech (Fagus sylvatica L.) seeds during alleviation of dormancy and in the early stage of germination

Cited by 6 publications

References 36 publications

Proteomics, Holm Oak (Quercus ilex L.) and Other Recalcitrant and Orphan Forest Tree Species: How do They See Each Other?

Proteomics, Holm Oak (Quercus ilex L.) and Other Recalcitrant and Orphan Forest Tree Species: How do They See Each Other?

Dehydration Sensitivity at the Early Seedling Establishment Stages of the European Beech (Fagus sylvatica L.)

Effects of storage on seed germination and viability for three native tree species of Ecuador

Contact Info

Product

Resources

About