Age-related changes in renal AQP3 and AQP4 expression in Sprague Dawley rats

Abstract

ABSTRACT. Aquaporin (AQP) 3 and AQP4 are important in urine concentrating mechanisms and in other physiological functions such as brain water balance, cell migration, cell proliferation, fat metabolism, and epidermal hydration. The results of studies investigating AQP3 and AQP4 expression in the kidneys are inconsistent, and systematic research is rare. This study aimed to obtain a better understanding of the changes in renal AQP3 and AQP4 mRNA expression that take place with age. The expression of AQP3 and AQP4 mRNA, during prenatal and postnatal development, and during aging, was investigated in kidneys from Sprague-Dawley rats. The pattern of AQP3 expression was similar to that of AQP4 expression during development, and both were detected at gestational day 19 in the rat kidney where they maintained a stable level to postnatal day 14. Subsequently, a significant increase in expression was observed from day 21 to day 35, with peak expression occurring at day 35. No significant change in AQP3 or AQP4 mRNA expression was observed after day 35, apart from AQP4, which increased at day 540. Moreover, the expression of both AQP3 and AQP4 on day 850 was higher than on day -2, and lower than on days 28 and 35. The expression of AQP3 and AQP4 was similar on days 1, 7, 14, and 21. These findings indicate that mRNA expression of AQP3 and AQP4 varies with age, which should be considered when treating kidney disease in pediatric and elderly patients.

LncRNA-miRNA-mRNA regulatory networks in skin aging and therapeutic potentials

“…They employed in silico analysis to identify photoaging-related genes, followed by the screening of differentially expressed lncRNAs and miRNAs to establish ceRNA interaction networks. Among the genes examined, AQP3 emerged as a noteworthy candidate ( Jing et al, 2016 ), exhibiting a negative correlation with aging in HDFs within one of the datasets. Subsequent experiments validated the role of AQP3 in enhancing HDF viability and mitigating senescence, primarily by impeding the ERK/p38 MAPK signaling pathway.…”

“…As a consequential outcome of this analysis, the study spotlighted ten hub genes, which include AQP4, TRPM8, TBR1, NTSR2, MPPED1, BARHL2, PAX9, CPN1, CES3, and CHGB. These hub genes were determined to play pivotal roles in orchestrating protein-protein interactions (PPIs) relevant to the progression or potential reversal of skin aging ( Jing et al, 2016 ; Bicakci et al, 2017 ; Ikarashi et al, 2017 ; Owasil et al, 2020 ; Thapa et al, 2021 ; Chen et al, 2022a ; Chen et al, 2022b ), underscoring their significance in the intricate biological processes involved.…”