Age-Related Changes in Serum/Plasma Biochemical Parameters of WHHLMI Rabbits.

Abstract

Abstract:We developed myocardial infarction-prone rabbits (WHHLMI rabbits) by selectively breeding coronary atherosclerosis-prone WHHL rabbits. To examine the serum/ plasma biochemical parameters of this animal model, we assayed the lipid and glucose levels, and enzyme activities of WHHLMI rabbits from 2 to 26 months of age using solid phase analysis. The results showed a good correlation with those measured with a conventional method. The serum enzyme activities and lipid levels varied with aging despite almost no change in the plasma glucose levels. Gender differences were observed in the total cholesterol, triglyceride, and lactate dehydrogenase activity levels. The data on these serum/plasma biochemical parameters will be useful in studies of myocardial infarction or pharmacological studies using this model. Key words: biochemical parameters, dry chemistry instrument, WHHLMI rabbitsWe have carried out pre-clinical studies of several compounds using WHHL rabbits, which show spontaneous hypercholesterolemia, hyperinsulinemia and atherosclerosis [4,12,15,16,21], to examine whether such compounds can reduce serum lipid levels, ameliorate insulin responses, or suppress atherosclerosis [13-15, 17-19, 22]. Although assays of serum/plasma biochemical parameters are important, there have been no reports on changes in these parameters with aging in this animal model or any other rabbit strain. Recently, we developed an animal model of spontaneous myocardial infarction (WHHLMI rabbit) by selectively breeding coronary atherosclerosis-prone WHHL rabbits [20]. This animal model suffers from myocardial infarction without any interventions and the findings of

Normal values of haematological and some biochemical parameters in serum and urine of New Zealand White rabbits

“…It is hypothesised that this difference may be due to the variations in some factors such as stress, blood collection methods and housing conditions. However, in a study (Yamada et al, 2004) with similar conditions to our work, no statistically significant difference for serum glucose levels was determined between male and female animals.…”

Análise da biocompatibilidade de cones de biovidro e biovitrocerâmico (Biosilicato®) em cavidade eviscerada de coelho

“…For biochemical tests, the values at M0 were used as a baseline and were then compared to the values at the time of sacrifice. Most biochemical results were in agreement with those found by other researchers, (9,17,18,21,22) with only physiological variations without clinical significance, as the final values were lower than the initial ones.…”

“…These changes characterise a healing process (23) , with replacement of damaged intraocular tissue by connective tissue. (9,22,23) The formation of a pseudocapsule was observed, and a reduction of its thickness was shown through morphometry, probably due to a reduction in oedema and in the number of inflammatory cells during the experiment. Comparison of the pseudocapsule's thickness with different biomaterials showed that the bioglass-ceramic FII group had a thicker pseudocapsule both 7 and 180 days after implantation, indicating that bioglass-ceramic FII caused a greater inflammatory reaction throughout the experiment.…”

Effect of Aging on Fatty Streak Formation in a Diet-Induced Mouse Model of Atherosclerosis

“…On both chow and atherogenic diets, old mice exhibited a significant reduction in plasma triglyceride levels relative to young mice. An age-related reduction in triglyceride levels has also been observed in male rabbits [16]. In contrast, in humans, aging is accompanied by increased levels of triglycerides, total cholesterol and non-HDL and decreased levels of HDL [17, 18].…”