Age-related changes in the gut microbiota and the core gut microbiome of healthy Thai humans

La-ongkham, Orawan; Nakphaichit, Massalin; Nakayama, Jiro; Keawsompong, Suttipun; Nitisinprasert, Sunee

doi:10.1007/s13205-020-02265-7

Cited by 40 publications

(24 citation statements)

References 63 publications

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“…Considering the majority of the samples from the 56 participants and the top ten bacterial families (see Table S4), we found two dominant families, namely, Lachnospiraceae and Ruminococcaceae in Thai adults. These two families are consistent with the findings of La-ongkham et al (2020) [22], whose study focused on the core taxonomic feature of the Thai gut microbiome.…”

Section: Assessment Of Taxonomic Profiles Of Gut Microbiome From Thaisupporting

confidence: 92%

“…For association analysis between demographic and clinical characteristics of the study cohort (i.e., age, gender and BMI), as well as the relative abundance of the dominant bacterial taxa across the 56 participants, the Spearman's rank correlation was applied using the "cor.test" command in the "stats" package version 4.0.2 in R. Using a threshold (p < 0.05), the strong, the moderate and the weak associations between the variables were defined by the absolute value of the correlation coefficient (r) ranges of 0.5-1.0, 0.3-0.49 and 0.1-0.29, respectively [22]. A scatter plot was used to display the correlation between the two variables (i.e., relative abundance and BMI), which was generated by using the "ggscatter" command in the "ggpubr" package version 0.4.0 in R.…”

Section: S Rrna Gene Sequencing Reads Processing and Microbial Commentioning

confidence: 99%

“…Loss of native gut microbiome has remarkably increased the risk of obesity and other chronic diseases in US immigrants compared to the Hmong people who currently live in Thailand [17]. Regarding Thai populations, the effects of different dietary habits and health conditions on the composition of gut microbiota have been previously investigated in different Thai cohorts of children, adults and elderly persons [18][19][20][21][22]. However, these analyses were only based on 16S rRNA gene sequencing data [23] and could not provide the full taxonomic information and metabolic function of gut microbiome.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults

Raethong

Nakphaichit

Suratannon

et al. 2021

Genes

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The gut microbiome plays a major role in the maintenance of human health. Characterizing the taxonomy and metabolic functions of the human gut microbiome is necessary for enhancing health. Here, we analyzed the metagenomic sequencing, assembly and construction of a meta-gene catalogue of the human gut microbiome with the overall aim of investigating the taxonomy and metabolic functions of the gut microbiome in Thai adults. As a result, the integrative analysis of 16S rRNA gene and whole metagenome shotgun (WMGS) sequencing data revealed that the dominant gut bacterial families were Lachnospiraceae and Ruminococcaceae of the Firmicutes phylum. Consistently, across 3.8 million (M) genes annotated from 163.5 gigabases (Gb) of WMGS sequencing data, a significant number of genes associated with carbohydrate metabolism of the dominant bacterial families were identified. Further identification of bacterial community-wide metabolic functions promisingly highlighted the importance of Roseburia and Faecalibacterium involvement in central carbon metabolism, sugar utilization and metabolism towards butyrate biosynthesis. This work presents an initial study of shotgun metagenomics in a Thai population-based cohort in a developing Southeast Asian country.

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Section: Assessment Of Taxonomic Profiles Of Gut Microbiome From Thaisupporting

confidence: 92%

Section: S Rrna Gene Sequencing Reads Processing and Microbial Commentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults

Raethong

Nakphaichit

Suratannon

et al. 2021

Genes

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“…Gut microorganisms, stress, and inflammation can modulate the permeability of the intestinal barrier and the BBB. Therefore, gut-brain bidirectional communication is variable depending on the state of the host and their lifestyle habits (Grenham et al, 2011;Claesson et al, 2012;Valle Gottlieb et al, 2018;La-Ongkham et al, 2020).…”

Section: The Gut-brain Axismentioning

confidence: 99%

Western Diet: Implications for Brain Function and Behavior

2020

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The Western diet (WD) pattern characterized by high daily intake of saturated fats and refined carbohydrates often leads to obesity and overweight, and it has been linked to cognitive impairment and emotional disorders in both animal models and humans. This dietary pattern alters the composition of gut microbiota, influencing brain function by different mechanisms involving the gut–brain axis. In addition, long-term exposure to highly palatable foods typical of WD could induce addictive-like eating behaviors and hypothalamic-pituitary-adrenal (HPA) axis dysregulation associated with chronic stress, anxiety, and depression. In turn, chronic stress modulates eating behavior, and it could have detrimental effects on different brain regions such as the hippocampus, hypothalamus, amygdala, and several cortical regions. Moreover, obesity and overweight induce neuroinflammation, causing neuronal dysfunction. In this review, we summarize the current scientific evidence about the mechanisms and factors relating WD consumption with altered brain function and behavior. Possible therapeutic interventions and limitations are also discussed, aiming to tackle and prevent this current pandemic.

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“…However, the microecological composition of each part is not uniform, and different parts of the gastrointestinal tract may have specific microecological communities [7]. Gender, obese body type, age, food, host genetic background, environment, antibacterial drugs, and other factors affect microbial structures [8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Comparative Study on the Esophageal Microflora of Different Tissue Types in Patients With Esophageal Squamous Cell Carcinoma

Liu¹,

Gao²,

Xu³

et al. 2021

Preprint

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Objective: This study aimed to compare the differences in microbiota between the postoperative tissues and esophageal mucosa tissues of patients with esophageal squamous cell carcinoma (ESCC). Methods: Seventy-two patients who had ESCC and diagnosed in Taihe Hospital were selected from July 2018 to July 2019 to participate in this work. Then, 27 postoperative tissues and 45 mucosa samples of ESCC were collected. The sequence V4 hypervariable region was amplified, and Illumina MiSeq sequencing was performed to analyze the differences between the two groups. Results: Results revealed that the Shannon and Chao1 indices of the postoperative esophageal cancer tissue group (Group A) were significantly higher (P<0.05) than those of the esophageal mucosa group (Group B). The Simpson index of Group A was higher than that of Group B, but the difference was not significant (P>0.05). The beta diversity of the two groups was also not significantly different (P>0.05). LEfSe analysis showed that the abundance of Megasphaera, Actinobacteria, Enterobacteriaceae, and Enterobacteriales in Group A was significantly higher than that in Group B, but the abundance of Mogibacteriaceae in Group B was significantly higher than that in Group A (P<0.05). At the phylum level, Actinobacteria and Verrucomicrobiae were more abundant in the postoperative tissue group than in the esophageal mucosa group. The abundance of Fusobacteriia, SR1, and Spirochaetes in the postoperative tissue group was significantly lower than that of the esophageal mucosa group(P<0.05). Conclusion: The source of the sample should be considered in studies on the esophageal flora. More relevant findings were obtained from postoperative tissues than from the normal mucosa.

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Age-related changes in the gut microbiota and the core gut microbiome of healthy Thai humans

Cited by 40 publications

References 63 publications

Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults

Analysis of Human Gut Microbiome: Taxonomy and Metabolic Functions in Thai Adults

Western Diet: Implications for Brain Function and Behavior

Comparative Study on the Esophageal Microflora of Different Tissue Types in Patients With Esophageal Squamous Cell Carcinoma

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