Age-related changes in the immunohistochemical localization pattern of α-smooth muscle actin and vimentin in rat testis

Moustafa, Amal M.

doi:10.1097/01.ehx.0000418134.14989.85

Cited by 4 publications

(7 citation statements)

References 32 publications

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“…Similarly, while Wang et al (2002) observed immunoreaction of vimentin in the Sertoli, Leydig and peritubular myoid cells of the Wistar rat testis. The immunolocalisation of vimentin in the vascular endothelium, Leydig and Sertoli cells in the African striped ground squirrel in the present study is also consistent with the findings in the testis of albino rats examined for vimentin by Moustafa (2012). However, Moustafa (2012) observed that the intensity of expression of vimentin in these testicular cells decreased with aging in the rat, and linked this to agerelated changes, such as reduction in testicular size, and decrease in Leydig cell steroidogenesis.…”

Section: Discussionsupporting

confidence: 91%

“…The immunolocalisation of vimentin in the vascular endothelium, Leydig and Sertoli cells in the African striped ground squirrel in the present study is also consistent with the findings in the testis of albino rats examined for vimentin by Moustafa (2012). However, Moustafa (2012) observed that the intensity of expression of vimentin in these testicular cells decreased with aging in the rat, and linked this to agerelated changes, such as reduction in testicular size, and decrease in Leydig cell steroidogenesis. In another study, Omirinde et al (2020) observed that the intensity of expression of vimentin in the epididymal cells decreased with age in the cane rat, and linked this to the presence of higher population of relatively undifferentiated cells in prepubertal cane rat relative to adult.…”

Section: Discussionsupporting

confidence: 91%

“…The present study has demonstrated the spatial distribution of some cytoskeletal proteins in the testis and epididymis in the African striped ground squirrel, as the first of its kind in this rodent. Research findings have demonstrated that cytoskeletal proteins such as desmin and vimentin compose the cytoskeleton of the mammalian testes, and are related to their structural support, among other functions (He et al, 2007;Sasaki et al, 2010;Moustafa, 2012). Peritubular myoid cells, surrounding the seminiferous tubules in the testis, have been found in all mammalian species, as a cytoskeletal layer of smooth muscle cells in rodents and stratified layer of smooth muscle in primates (Maekawa et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

“…Although vimentin was first described in a limited number of physiological and pathophysiological contexts, 109 more recent findings have suggested that it has diverse roles across a broad range of cell and tissue functions and is coupled to a large variety of human diseases (Satelli & Li,201). It has been expressed in different parts of the testis of rats, with evidence of decreased intensity as the rats advanced in age (Moustafa, 2012). The protein S-100 is so named because it is soluble in a 100% saturated solution of ammonium sulphate.…”

Section: Introductionmentioning

confidence: 99%

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Immunolocalization of Desmin, Vimentin and S-100 proteins in testis and epididymis of African striped ground squirrel (Xerus erythropus)

2023

JoSVAS

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The aim of the study was to immunolocalize desmin, vimentin and S-100 proteins in the testes and epididymis of African striped ground squirrel (Xerus erythropus) and establish their spatial distribution in the two organs. Formalin fixed-paraffin-embedded sections of the testis and epididymis obtained from ten apparently healthy adult male African striped ground squirrels were processed routinely for immunohistochemistry, using primary antibodies specific to desmin, vimentin and S-100. S-100The results showed that desmin reacted intensely in the myoid cells of the seminiferous tubules and smooth muscle cells of the epididymal ducts. It showed a moderate positive immunoreaction in the interstitial cells of Leydig, and the epididymal inter-ductal loose connective tissue. However, there was no immunoreaction of desmin in the spermatogonia or any other spermatogenic cell of the seminiferous tubule. Vimentin reacted intensely in the Leydig cells and spermatogonia. It showed moderate positive reaction in the myoid cells and the epididymal inter-ductal loose connective tissue. There was no immunoreaction of vimentin in the other spermatogenic cells (other than the spermatogonia). The S-100 proteins expressed a mild positive immunoreaction at the interstitial cells of Leydig, but negative immunoreaction in all other parts of testis and epididymis. Also, there was intense immunoreaction of desmin and vimentin and moderate immunoreaction of S-100 in the vascular endothelium in the testis and epididymis. In conclusion, the spatial distribution of desmin, vimentin and S-100 proteins in the testes and epididymis in the African striped ground squirrel showed some similarities and contrast with other mammals, giving insight into the functions of the proteins in these organs of the rodent.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Immunolocalization of Desmin, Vimentin and S-100 proteins in testis and epididymis of African striped ground squirrel (Xerus erythropus)

2023

JoSVAS

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“…Alpha smooth muscle actin (α-SMA) is a component of the cytoskeleton and a specific marker for myoepithelial and smooth muscle cells [ 81 , 82 ]. It affects nearly all tissues in the body, such as the liver, lung, testis, kidney, heart, and pancreas [ 83 ].…”

Section: Discussionmentioning

confidence: 99%

Olive Leaf Extract Attenuates Chlorpyrifos-Induced Neuro- and Reproductive Toxicity in Male Albino Rats

Hassan

Salah

Fahmy

et al. 2022

Life

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Chlorpyrifos (CPF) is a common organophosphorus insecticide. It is associated with negative consequences such as neurotoxicity and reproductive injury. This study aimed to observe the ability of olive leaf extract to attenuate chlorpyrifos toxicity, which induced neuro- and reproductive toxicity in male albino rats. Olive leaf extract (OLE) exhibits potent antioxidant and antiapoptotic properties. Twenty-two mature male rats were divided into four groups: control (saline), CPF (9 mg/kg), OLE (150 mg/kg), and CPF + OLE. Treatment was administered orally for 80 days. The CPF significantly reduced serum sex hormones, sperm counts and motility, high oxidants (MDA), and depleted antioxidants (GSH, SOD, TAC) in the brain and testes homogenate; additionally, it decreased serum AChE and brain neurotransmitters, increased Bax, decreased Bcl-2, and boosted caspase-3 immune expression in neural and testicular cells. Immunological expression of Ki 67 in the cerebrum, cerebellum, choroid plexus, and hippocampus was reduced, and α-SMA in testicular tissue also decreased. Histopathological findings were consistent with the above impacts. OLE co-administration significantly normalized all these abnormalities. OLE showed significant protection against neural and reproductive damage caused by CPF.

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Formalin Versus Bouin Solution for Rat Testicular Tissue Fixation: A Histochemical and Immunohistochemical Evaluation

Waleed Aziz,

Ghassan Saeed,

Turath Tawfeeq

2023

IJMTFM

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Background: An accurate histopathological assessment and reporting of testicular biopsies require an appropriate tissue fixative. We assessed the histological, histochemical, and immunohistochemical quality of testicular biopsies, comparing 10% formalin versus Bouin solution as tissue fixatives. Methods: This experimental study utilized 20 adult male albino rats equally divided into five cages for 30 days. By the end of the experiment, all animals were anesthetized, and both testes were removed and weighted; one testicle was fixed in 10% formalin and the other testicle in Bouin solution, offering 40 specimens and then subjected to histological, morphometric, histochemical, and immunohistochemical assessments. Results: Formalin revealed high-quality cytological details and better nuclear chromatin detail (P=0.03). At the architectural level, the Bouin solution showed better quality details with less cytoplasmic shrinkage of seminiferous tubule germ cells (P=0.001). Bouin’s fixed tissues were more suitable for staining by trichrome methods but unsuitable when subsequent immunohistochemistry was requested. The diagnostic concordance between the Bouin solution versus formalin-fixed biopsies was 91.7%. Conclusion: This study supports that the morphology of testicular tissue fixed with Bouin solution was nearly comparable to those fixed with 10% neutral buffered formalin. However, the Bouin solution cannot substitute formalin when subsequent immunohistochemistry is considered.

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Age-related changes in the immunohistochemical localization pattern of α-smooth muscle actin and vimentin in rat testis

Cited by 4 publications

References 32 publications

Immunolocalization of Desmin, Vimentin and S-100 proteins in testis and epididymis of African striped ground squirrel (Xerus erythropus)

Immunolocalization of Desmin, Vimentin and S-100 proteins in testis and epididymis of African striped ground squirrel (Xerus erythropus)

Olive Leaf Extract Attenuates Chlorpyrifos-Induced Neuro- and Reproductive Toxicity in Male Albino Rats

Formalin Versus Bouin Solution for Rat Testicular Tissue Fixation: A Histochemical and Immunohistochemical Evaluation

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