Age-related changes of aquaporin expression patterns in the postnatal rat retina

Ortak, Hüseyin; Çaylı, Sevil; Ocaklı, Seda; Söğüt, Erkan; Ekici, Filiz; Taş, Ufuk; Demir, Selim

doi:10.1016/j.acthis.2012.09.013

Cited by 15 publications

(14 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Second, a punctate granular pattern is observed in the OPL much of which co-localizes with AQP4 staining [117,122]. AQP6 expression was also detected in adult mice in the RPE, ONL, OPL, INL, GCL and ILM layers [137]. In addition to being a water channel, AQP6 also functions as an anion channel with a high permeability for nitrate [138,139].…”

Section: Retinamentioning

confidence: 99%

Aquaporins in the eye: Expression, function, and roles in ocular disease

Schey

Wang

Wenke

et al. 2014

Biochimica et Biophysica Acta (BBA) - General Subjects

113

100

View full text Add to dashboard Cite

Background All thirteen known mammalian aquaporins have been detected in the eye. Moreover, aquaporins have been identified as playing essential roles in ocular functions ranging from maintenance of lens and corneal transparency to production of aqueous humor to maintenance of cellular homeostasis and regulation of signal transduction in the retina. Scope of review This review summarizes the expression and known functions of ocular aquaporins and discusses their known and potential roles in ocular diseases. Major conclusions Aquaporins play essential roles in all ocular tissues. Remarkably, not all aquaporin function as a water permeable channel and the functions of many aquaporins in ocular tissues remain unknown. Given their vital roles in maintaining ocular function and their roles in disease, aquaporins represent potential targets for future therapeutic development. General significance Since aquaporins play key roles in ocular physiology, an understanding of these functions is important to improving ocular health and treating diseases of the eye. It is likely that future therapies for ocular diseases will rely on modulation of aquaporin expression and/or function. This article is part of a Special Issue entitled Aquaporins.

show abstract

Section: Retinamentioning

confidence: 99%

Aquaporins in the eye: Expression, function, and roles in ocular disease

Schey

Wang

Wenke

et al. 2014

Biochimica et Biophysica Acta (BBA) - General Subjects

113

100

View full text Add to dashboard Cite

show abstract

“…However, the finding of significant differences using a limited number of eyes supports that the results are valid. The finding that AQP1 expression is associated with drusen size suggests an important role for this water channel in the development of outer retinal disease, perhaps as a dynamic mechanism that can be turned on and off during the development of the disease [20] or trafficked to a different anatomical position, as has been observed for AQP2 in the renal tubules [21]. …”

Section: Discussionmentioning

confidence: 99%

“…However, it is unknown whether the movement of water in the outer retina is also facilitated by AQPs. Evidence from studies of embryonic development suggests that RPE cells express AQP1, but the expression of this isotype of water channel gradually decreases with age and disappears as the RPE cells mature [7]. Interestingly, AQP1 has not been found in normal RPE cells from the fully developed eye [8] but only in cultured RPE cells [9].…”

Section: Introductionmentioning

confidence: 99%

Aquaporin-1 Expression in Retinal Pigment Epithelial Cells Overlying Retinal Drusen

Tran¹,

Bek

Cour

et al. 2016

Ophthalmic Res

View full text Add to dashboard Cite

Purpose: In the outer retina, age-related macular degeneration (AMD) results in reduced hydraulic conductivity in Bruch's membrane, possibly leading to altered water transport in retinal pigment epithelial (RPE) cells. We hypothesize that RPE cells may express aquaporin-1 (AQP1) to compensate for these changes. Therefore, we wanted to investigate the expression of AQP1 in RPE cells of human eyes with age-related maculopathy (ARM) and AMD, and eyes with tumour-associated drusen. Methods: Nine human eyes with ARM, 6 eyes with AMD and 9 eyes with choroidal malignant melanoma were examined for immunoreactivity to AQP1. AQP1 labelling in the RPE cells was evaluated for each drusen and grouped according to size and AQP1 labelling. AQP1 labelling in the RPE outside drusen was also evaluated. Results: AQP1 labelling was observed in the apical membrane of the RPE cells situated above drusen in all three groups. There was a significant association between AQP1 labelling and drusen size (p < 0.001), and AQP1 labelling was more frequently observed in large drusen. Conclusion: AQP1 was expressed in RPE cells covering drusen but not in RPE cells outside drusen. We suggest that AQP1 expression is upregulated in the cell membranes of RPE cells above drusen in order to alleviate the increased need for fluid transport across the growing drusen.

show abstract

“…Expression of aquaporin in various tissues changes with age. As a result of aging, changing expressions of AQP2 and AQP3 in the renal medulla [25], AQP1 and AQP3 in the intervertebral disc tissue [12,19], AQP1 in skin and in the choroid plexus of brain ventricles [26], AQP4 in the brain and the cochlea [27], AQP1 and AQP5 in the lungs [28], AQP1, AQP3, AQP4, AQP6, and AQP9 in the retina [29] were demonstrated. In our study AQP1 and AQP3 expression decreased with aging.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Age-Related Changes in Expression of Aquaporin-1 and Aquaporin-3 in Rat Bone

Sapmaz¹

2016

Ann Clin Anal Med

View full text Add to dashboard Cite

ÖzetAmaç: Aquaporinler su ve iyon transportu sağlayan membran kanal proteinleridir. Yaşlanma ile dokulardaki aquaporinlerin miktarı değişmektedir. Aquaporin-1 (AQP1) ve aquaporin-3 (AQP3)'ün kıkırdak dokuda yaşlanma ile azaldığı gösterilmiştir. Ancak kemik dokuda yaşlanma ile aquaporinlerin miktarında değişim gösterilmemiştir. Bu çalışmada genç ve yaşlı hayvanların kemik dokularında AQP1, AQP3 ve tip I kollajen ekspresyonlarının immünohis-tokimyasal olarak araştırılması amaçlandı. Gereç ve Yöntem: Bu çalışmada 14 adet Wistar Albino cinsi sıçan kullanıldı. Grup I (n=7) iki aylık genç sıçan-lardan, Grup II (n=7) onsekiz aylık yaşlı sıçanlardan oluşmaktaydı. Kemik dokusu (femur) histopatolojik ve immunohistokimyasal değerlendirme için alın-dı. Rutin histolojik prosedürün ardından parafine gömülen dokulardan 4-5 µm kalınlığında kesitler alındı. İmmunohistokimyasal olarak AQP1, AQP3 ve tip I kollajen boyama ayrıca Hematoksilen-eozin boyama yapıldı. Bulgular: Genç ve yaşlı sıçanlarda immünohistokimyasal olarak AQP1, AQP3 ve tip I kollajen ekspresyonu gösterildi. Yaşlanma ile AQP1, AQP3 ve tip I kollajen miktarında azalma olduğu gözlendi. Tartışma: AQP1 ve AQP3 mik tarındaki azalmanın kemik dokuda yaşa bağlı değişikliklerle ilgili olabilece ği kanaatindeyiz. Anahtar KelimelerSıçan; Kemik Dokusu; Yaşlanma; Aquaporin 1; Aquaporin 3 Özet Aim: Aquaporins are membrane channel proteins that transport water and ions. The amount of aquaporins in tissue changes with age. The amount of aquaporin-1 (AQP1) and aquaporin-3 (AQP3) is considered to decrease in cartilage tissue with age. However, no age-related change has been reported regarding the amount of aquaporins in bone tissue. In this study, our aim was to examine expressions of AQP1, AQP3, and type I collagen immunohistochemically in the bone tissues of young and old rats. Material and Method: Fourteen Wistar Albino rats were included in this study. Group I (n=7) consisted of young rats that were two months old, while Group II (n=7) consisted of old rats that were eighteen months old. Bone tissue (femur) was dissected and examined histopathologically and immunohistochemically. After routine histological procedure, sections at 4-5 µm thickness were obtained from tissues embedded in paraffin. Sections were stained immunohistochemically for AQP1, AQP3, and type I collagen as well as with hematoxylin-eosin. Results: Immunohistochemically, expressions of AQP1, AQP3, and type I collagen were demonstrated in both young and old rats. AQP1, AQP3, and type I collagen amounts were found to decrease with aging. Discussion: Our findings suggest that reduced amounts of AQP1 and AQP3 may be related to agerelated changes in bone tissue.

show abstract

Age-related changes of aquaporin expression patterns in the postnatal rat retina

Cited by 15 publications

References 32 publications

Aquaporins in the eye: Expression, function, and roles in ocular disease

Aquaporins in the eye: Expression, function, and roles in ocular disease

Aquaporin-1 Expression in Retinal Pigment Epithelial Cells Overlying Retinal Drusen

Investigation of Age-Related Changes in Expression of Aquaporin-1 and Aquaporin-3 in Rat Bone

Contact Info

Product

Resources

About