Age-related changes of cell outgrowth from rat calvarial and mandibular bone in vitro

Cei, Silvia; Mair, Birgit; Kandler, Barbara; Gabriele, Mario; Watzek, Georg; Gruber, Reinhard

doi:10.1016/j.jcms.2006.07.856

Cited by 7 publications

(18 citation statements)

References 27 publications

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“…and temperatures. The peak melting temperature of PLLA blocks shows a small decrease upon increasing PCL content within the block copolymers and with respect to neat PLLA 24 . Glass transitions of PLLA blocks can also be seen in Figure 1, where they can easily be recognized as an endothermic step with a small enthalpic relaxation peak (which can, in some cases, overlap with PCL melting endotherms), and they decreased as the content of PCL increased, indicating a degree of miscibility between the components.…”

Section: Resultsmentioning

confidence: 92%

“…The degree of crystallinity was calculated and presented in Table I. Remarkable differences in thermal behavior can be seen for the two PLLA homopolymers employed here. PLLA 86 has lower T g and T m values (see Table I) than PLLA 24 suggesting that the stereoregularity in PLLA 86 is not as high as in PLLA 24 as far as the content of the L stereoisomer is concerned (a fraction of D isomer is probably present in the sample). This can also explain why PLLA 86 has a much lower crystallization rate than PLLA 24 .…”

Section: Lists the Observable Transition Enthalpiesmentioning

confidence: 98%

“…Calvarias were treated as described previously and cell migration from bone explants was measured as described previously. 24 Briefly, extracted calvaria were cut into pieces of approximately (2 3 2) mm 2 . Bone pieces were placed on the bottom of a 96 wells tissue culture dish in which the different test substrates were previously placed.…”

Section: Explants Culturementioning

confidence: 99%

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Evaluation of cell affinity on poly(L‐lactide) and poly(ε‐caprolactone) blends and on PLLA‐b‐PCL diblock copolymer surfaces

Ajami-Henriquez

Rodríguez

Sabino

et al. 2008

J Biomedical Materials Res

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An evaluation of cell proliferation and adhesion on biocompatible film supports was performed. A series of films were compression molded from commercially available poly (L-lactide), PLLA, and poly(epsilon-caprolactone), PCL, and from their melt mixed blends (PLLA/PCL blends). These were compared with compression molded films of PLLA-b-PCL model diblock copolymers. The samples were analyzed by differential scanning calorimetry (DSC), contact angle measurements, and scanning force microscopy (SFM). Cell adhesion and proliferation were performed with monkey derived fibroblasts (VERO) and with osteoblastic cells obtained either enzymatically or from explants cultures of Sprague-Dawley rat calvaria. Migration studies were performed with bone explants of the same origin. The results obtained indicate that although all materials tested were suitable for the support of cellular growth, a PLLA-b-PCL diblock copolymer sample with 93% PLLA was significantly more efficient. This sample exhibited a unique surface morphology with long range ordered domains (of the order of 2-3 mum) of edge-on PLLA lamellae that can promote "cell contact guidance." The influence of other factors such as chemical composition, degree of crystallinity, and surface roughness did not play a major role in determining cell preference toward a specific surface for the materials employed in this work.

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Section: Resultsmentioning

confidence: 92%

Section: Lists the Observable Transition Enthalpiesmentioning

confidence: 98%

Section: Explants Culturementioning

confidence: 99%

See 1 more Smart Citation

Evaluation of cell affinity on poly(L‐lactide) and poly(ε‐caprolactone) blends and on PLLA‐b‐PCL diblock copolymer surfaces

Ajami-Henriquez

Rodríguez

Sabino

et al. 2008

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…Therefore, the combination of the osteoconductive porous scaffold and the osteoinductive molecules with appropriate carriers such as BMP and HAP can enhance bone induction (Spector et al, 2001), which was the original hypothesis of this study (Yoshida et al, 1999). Age plays a role in cytokine release in both humans and animals where young individuals are known to heal at a rapid rate compared to an adult organism (Aalami et al, 2004;Cei et al, 2006). Rats are considered to be mature at 6e8 weeks, but, at 20 weeks, they show a delay in healing.…”

Section: Discussionmentioning

confidence: 95%

Bone regeneration with BMP-2 and hydroxyapatite in critical-size calvarial defects in rats

Notodihardjo

Kakudo

Kushida

et al. 2012

Journal of Cranio-Maxillofacial Surgery

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“…The difficulties with bony regeneration in the adolescent and adult skull [1, 2] have prompted surgeons to seek new biomaterials to hasten the ossification process. A number of biomaterials such as demineralized bone, hydroxyapatite, polymer composites and acrylic resins have been utilized but with only limited success.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Reversibly Immortalized Calvarial Mesenchymal Progenitor Cells

Shenaq

Teven

Seitz

et al. 2015

Journal of Craniofacial Surgery

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Background Bone morphogenetic proteins (BMPs) play a sentinel role in osteoblastic differentiation, and their implementation into clinical practice can revolutionize cranial reconstruction. Preliminary data suggest a therapeutic role of adenoviral gene delivery of BMPs in murine calvarial defect healing. Poor transgene expression inherent in direct adenoviral therapy prompted investigation of cell-based strategies. Objective To isolate and immortalize calvarial cells as a potential progenitor source for osseous tissue engineering. Materials & Methods Cells were isolated from murine skulls, cultured, and transduced with a retroviral vector bearing the loxP-flanked SV40 large T antigen. Immortalized calvarial cells (iCALs) were evaluated via light microscopy, immunohistochemistry, and flow cytometry to determine whether the immortalization process altered cell morphology or progenitor cell profile. iCALs were then infected with adenoviral vectors encoding BMP-2 or GFP and assessed for early and late stages of osteogenic differentiation. Results Immortalization of calvarial cells did not alter cell morphology as demonstrated by phase contrast microscopy. Mesenchymal progenitor cell markers CD166, CD73, CD44, and CD105 were detected at varying levels in both primary cells and iCALs. Significant elevations in alkaline phosphatase activity, osteocalcin mRNA transcription, and matrix mineralization were detected in BMP-2 treated iCALs compared to GFP treated cells. Gross and histological analyses revealed ectopic bone production from treated cells compared to controls in an in vivo stem cell implantation assay. Conclusion We have established an immortalized osteoprogenitor cell line from juvenile calvarial cells that retain a progenitor cell phenotype and can successfully undergo osteogenic differentiation upon BMP-2 stimulation. These cells provide a valuable platform to investigate the molecular mechanisms underlying intramembranous bone formation and to screen for factors/small molecules that can facilitate the healing of osseous defects in the craniofacial skeleton.

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Age-related changes of cell outgrowth from rat calvarial and mandibular bone in vitro

Cited by 7 publications

References 27 publications

Evaluation of cell affinity on poly(L‐lactide) and poly(ε‐caprolactone) blends and on PLLA‐b‐PCL diblock copolymer surfaces

Evaluation of cell affinity on poly(L‐lactide) and poly(ε‐caprolactone) blends and on PLLA‐b‐PCL diblock copolymer surfaces

Bone regeneration with BMP-2 and hydroxyapatite in critical-size calvarial defects in rats

Characterization of Reversibly Immortalized Calvarial Mesenchymal Progenitor Cells

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