Age related changes of neuropeptide Y-ergic system in the rat duodenum

Budnik, A. F.; Vyshnyakova, Polina; Maslyukov, P. M.

doi:10.1016/j.npep.2019.101982

Cited by 6 publications

(8 citation statements)

References 45 publications

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“…In our work, only a small percentage of MP nNOS-IR neurons expressed NPY regardless of age. In accordance with previous works, the percentage of NPY-IR neurons is small in the MP during postnatal ontogenesis (Budnik et al, 2020).…”

Section: Discussionsupporting

confidence: 93%

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Postnatal development of the enteric neurons expressing neuronal nitric oxide synthase

Budnik

Maslyukov

2022

The Anatomical Record

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Neurons, expressing neuronal nitric oxide synthase (nNOS) in the enteric ganglia are inhibitory motor neurons or interneurons. The aim of the study was to identify the percentage, cross‐sectional area of nNOS‐immunoreactive (IR) neurons and their colocalization with choline acetyltransferase (ChAT), vasoactive intestinal polypeptide (VIP), and neuropeptide Y in the intramural ganglia of the myenteric (MP) and submucous plexus (SP) of the small intestine (SI) and large intestine (LI) of rats of different age groups using immunohistochemical methods. In the intramural ganglia of the MP, the largest percentage of nNOS‐IR neurons was detected in newborn rats in the LI (81 ± 0.9%) and SI (48 ± 4.1%). Subsequently, it decreased in ontogenesis up to 60 days of life (26 ± 0.9% LI, 29 ± 3.2% SI), and did not change until senescence. In the SP, abundant nNOS‐IR neurons were also detected in newborns (82 ± 7.0% SI, 85 ± 3.2% LI), while their percentage decreased significantly in the next 20 days. Furthermore, a very small number of nNOS‐IR neurons was detected in 30‐day‐ and 2‐month‐old animals, but they again appeared in large numbers in aged rats. In the MP, the highest percentage of nNOS+/ChAT+ neurons was in 1‐day‐old, 10‐day‐old, and 2‐year‐old rats. In the SP, the largest number of nNOS‐IR neurons colocalized ChAT regardless of age. In the MP of all rats, many nNOS‐IR neurons colocalized VIP, and the maximal percentage of nNOS+/VIP+ neurons was found in 2‐year‐old rats, minimal—in newborns. In conclusion, nNOS expression in neurons of the gut is decreased in early postnatal ontogenesis and subsequently increased in aged rats.

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Section: Discussionsupporting

confidence: 93%

“…In contrast to the MP, a high percentage of SP nNOS-IR neurons colocalized NPY. Previous studies showed that SP contained a large number of neurons expressing NPY and VIP (Budnik et al, 2020;Furness, 2006).…”

Section: Discussionmentioning

confidence: 99%

Postnatal development of the enteric neurons expressing neuronal nitric oxide synthase

Budnik

Maslyukov

2022

The Anatomical Record

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“…These data suggest a similar age-related gastro-intestinal regulation of NPY in vertebrates and could be relevant for translational studies. In addition, we observed that NPY is also localized in the fibers of the myenteric plexus of both species without any age-related changes, accordingly to recent data reported in mammals [ 43 ]. NPY immunoreactive neurons in the myenteric plexus did not change significantly in the duodenum of rats at diverse ages (1, 10, 20, 30, 60-days-old and 2-years-old), which is different from what happens in the submucosal plexus where they clearly decreased from young (30 days old) to old (2-years) rats [ 43 ].…”

Section: Discussionsupporting

confidence: 90%

“…In addition, we observed that NPY is also localized in the fibers of the myenteric plexus of both species without any age-related changes, accordingly to recent data reported in mammals [ 43 ]. NPY immunoreactive neurons in the myenteric plexus did not change significantly in the duodenum of rats at diverse ages (1, 10, 20, 30, 60-days-old and 2-years-old), which is different from what happens in the submucosal plexus where they clearly decreased from young (30 days old) to old (2-years) rats [ 43 ]. It is noteworthy that we did not observe immunostaining in the submucous plexus either in turquoise killifish or in zebrafish.…”

Section: Discussionsupporting

confidence: 90%

Central and Peripheral NPY Age-Related Regulation: A Comparative Analysis in Fish Translational Models

Giaquinto

Felice

Attanasio

et al. 2022

IJMS

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NPY is among the most abundant neuropeptides in vertebrate brain and is primarily involved in the regulation of food intake. The NPY system is also associated with the aging process showing beneficial effects on neuronal survival via autophagy modulation. Here, we explore the age-related regulation of NPY in the brain and foregut of the shortest- and longest-lived fish species, Nothobranchius furzeri and Danio rerio, respectively. These two research models, despite some similarities, display profound biological differences making them attractive vertebrates to elucidate the mechanisms underlying the regulation of neuropeptide synthesis and function. It is noteworthy that in both fish species only Npya has been identified, while in the other teleosts two classes of NPY (Npya and Npyb) have been annotated. Our findings document that in both species: i) NPY is centrally regulated; ii) NPY levels increase in the brain during aging; iii) NPY is localized in the enteroendocrine cells as well as in the myenteric plexus and drastically decreases in old animals. According to our data, the age-related regulation in the gut resembles that described in other vertebrate species while the increased levels in the brain offer the unique possibility to explore the role of NPY in model organisms to develop future experimental and translatable approaches.

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“…The number and localization of enteric plexuses depend on the mammal species in question. In rodent intestines, two types of enteric plexuses are present, i.e., the myenteric plexus (MP) in the intestinal muscular layer and submucous plexus, near the lamina propria of the mucosa [ 14 ]. In turn, in large mammal species and humans, the submucous plexus is divided into two plexuses: the inner submucous plexus, located in the same place as the submucous plexus in rodents, and the outer submucous plexus, located near the side of the circular muscle layer [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

The Influence of Bisphenol A (BPA) on the Occurrence of Selected Active Substances in Neuregulin 1 (NRG1)-Positive Enteric Neurons in the Porcine Large Intestine

Makowska

Szymańska

Całka

et al. 2021

IJMS

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Bisphenol A (BPA) is a substance used in the manufacture of plastics which shows multidirectional adverse effects on living organisms. Since the main path of intoxication with BPA is via the gastrointestinal (GI) tract, the stomach and intestine are especially vulnerable to the impact of this substance. One of the main factors participating in the regulation of intestinal functions is the enteric nervous system (ENS), which is characterized by high neurochemical diversity. Neuregulin 1 (NRG1) is one of the lesser-known active substances in the ENS. During the present study (performed using the double immunofluorescence method), the co-localization of NRG1 with other neuronal substances in the ENS of the caecum and the ascending and descending colon has been investigated under physiological conditions and after the administration of BPA. The obtained results indicate that NRG1-positive neurons also contain substance P, vasoactive intestinal polypeptide, a neuronal isoform of nitric oxide synthase and galanin and the degree of each co-localization depend on the type of enteric plexus and the particular fragment of the intestine. Moreover, it has been shown that BPA generally increases the degree of co-localization of NRG1 with other substances.

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Age related changes of neuropeptide Y-ergic system in the rat duodenum

Cited by 6 publications

References 45 publications

Postnatal development of the enteric neurons expressing neuronal nitric oxide synthase

Postnatal development of the enteric neurons expressing neuronal nitric oxide synthase

Central and Peripheral NPY Age-Related Regulation: A Comparative Analysis in Fish Translational Models

The Influence of Bisphenol A (BPA) on the Occurrence of Selected Active Substances in Neuregulin 1 (NRG1)-Positive Enteric Neurons in the Porcine Large Intestine

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