Age-Related Changes of the Somatotropic Axis in Cloned Holstein Calves1

Govoni, Kristen E; Tian, X.C.; Kazmer, G.W.; Taneja, M.; Enright, Brian; Rivard, Amber L.; Yang, Xiangzhong; Zinn, S. A.

doi:10.1095/biolreprod66.5.1293

Cited by 38 publications

(24 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The prepubertal concentrations of metabolic hormones and metabolites in these same animals measured at 6 months of age (GH, 3-47 ng/ml; IGF-I, 110-284 ng/ ml; insulin, fasted 0·2-0·97 ng/ml, fed 0·2-2·39 ng/ml; glucose, fasted 3·8-6·3 mmol/l, fed 4·0-6·1 mmol/l; NEFA, fasted 29-447 µmol/l, fed 20-85 µmol/l) were within the ranges reported for Holstein calves of a similar age in previous studies (Lapierre et al 1992, Govoni et al 2002. No significant associations were found between the physical parameters measured at 6 months of age (weight, height, girth, body condition score (BCS), liveweight gain from birth) and the hormone or metabolite concentrations measured at the same time.…”

Section: Prepubertysupporting

confidence: 85%

“…Following birth, the GH-IGF axis in dairy calves is functional but feedback mechanisms are still developing. GH concentrations decrease post-puberty (Govoni et al 2002) and the response to GH-releasing factor (GRF) decreases (Lapierre et al 1992). A further complication is that the relationship between GH and IGF-I uncouples in physiological states of compromised nutrition or disease so that concentrations of IGF-I do not increase, as expected, despite increases in GH (GH resistance;Thissen et al 1994, Van den Berghe et al 1998).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

First lactation ovarian function in dairy heifers in relation to prepubertal metabolic profiles

Taylor¹,

Beever²,

Bryant³

et al. 2004

Journal of Endocrinology

View full text Add to dashboard Cite

The aim of this study was to determine whether any differences in the GH-IGF-I axis in juvenile calves were predictive of fertility problems as adult cows. Endogenous metabolic hormone profiles before and after feeding and the response to a GH-releasing factor (GRF) challenge were measured in prepubertal (6 month) dairy calves. These metabolic parameters were subsequently related to physical characteristics at puberty and to ovarian function during the first lactation. Milk progesterone analysis was used to categorize the animals into those with normal progesterone profiles following calving (n=17) and those that developed delayed ovulation (DOV1, n=9) or persistent corpus luteum (PCL1, n=6) profiles. There were associations between prepubertal GH parameters, glucose and non-esterified fatty acid (NEFA) concentrations and the body condition score at which the animals attained puberty. The calves which subsequently developed DOV1 profiles as cows tended to have a higher GH pulse amplitude during fasting than normal profile animals, they did not show the anticipated decrease in circulating glucose concentrations following a postprandial rise in insulin and they also had the lowest IGF-I concentrations. The calves that later developed PCL1 had a significantly larger GH pulse amplitude and pulse area than normal profile animals in the fed period and had the highest IGF-I concentrations. There were no differences in prepubertal insulin or NEFA concentrations or in the GH response to a GRF challenge between the different progesterone profile categories. Plasma IGF-I concentrations in prepubertal animals were positively correlated with their post-calving concentrations, whereas glucose concentrations had a negative correlation between these time-periods. These results suggested that the different juvenile endocrine profiles of the DOV1 cows may predispose them to a higher rate of tissue mobilization during lactation and a consequent reduction in fertility, while altered GH and IGF-I levels in PCL1 cows may later contribute to the maintenance of the persistent corpus luteum. Therefore metabolic differences in prepubertal calves were later reflected by altered reproductive function during the first lactation.

show abstract

Section: Prepubertysupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

First lactation ovarian function in dairy heifers in relation to prepubertal metabolic profiles

Taylor¹,

Beever²,

Bryant³

et al. 2004

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…In canids, SCNT has been applied not only to the cloning of companion dogs [12,13,16] but also to the preservation of endangered canine species [14,18]. In some species, cloned offspring produced by SCNT have been reported to show normal reproductive characteristics and successfully produce progeny by natural mating [1,2,6,7,21]. To our knowledge, however, there are no studies on the reproductive characteristics of cloned dogs.…”

mentioning

confidence: 99%

Serum Levels of Reproductive Hormones and Ultrasonographic Monitoring of Ovarian Follicles in Female Cloned Dogs

Hong

Park

et al. 2010

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. This study investigated the reproductive characteristics in 2 cloned female beagle dogs (Clones A and B) during proestrus and early estrus. The pre-ovulatory estradiol peak occurred 2 days before the pre-ovulatory luteinizing hormone (LH) surge, while the follicle stimulating hormone surge started concomitantly with the LH surge in both cloned dogs. Serum progesterone levels increased during proestrus and estrus and its concentration on the day of the LH surge was 3.59 and 2.71 ng/ml in Clones A and B, respectively. Gradual follicular growth was observed by ultrasonography during proestrus. Although the numbers are limited, these cloned female dogs showed normal ranges of reproductive hormone levels and follicular changes during proestrous and early estrous stages of the cycle.KEY WORDS: cloned dog, estrous cycle, puberty, reproductive endocrinology/hormone, ultrasonography.J. Vet. Med. Sci. 72(1): 89-92, 2010 Cloning technology using somatic cell nuclear transfer (SCNT) provides valuable opportunities for the fields of biomedical science, agriculture and preservation of endangered animals [2,6,17]. In canids, SCNT has been applied not only to the cloning of companion dogs [12,13,16] but also to the preservation of endangered canine species [14,18]. In some species, cloned offspring produced by SCNT have been reported to show normal reproductive characteristics and successfully produce progeny by natural mating [1,2,6,7,21]. To our knowledge, however, there are no studies on the reproductive characteristics of cloned dogs. Accordingly, this study was designed to investigate systemic reproductive characteristics of cloned beagles, specifically their hormonal profiles and ovarian follicular development.Recently, we produced two cloned beagles (designated as Clones A and B) derived from canine fetal fibroblasts [10]. For this study, they were monitored regularly from six months of age for the presence of vulvar swelling and serosanguinous vaginal discharge, which were used as indications of the onset of proestrus. From the first day of proestrus, jugular venous blood samples were collected twice a day for hormonal analysis. Serum was prepared by centrifuging at 3,000 rpm for 20 min and stored at -30C until use for hormone measurement. Additionally, to gain more detailed information regarding the changes of hormones around the time of ovulation, blood samples were collected three times a day after the serum progesterone concentration exceeded 1 ng/ml. Previous studies demonstrated that progesterone can be used to accurately predict ovulation in canines [11,14].For analyzing reproductive hormones, progesterone, estradiol-17 (E 2 ), follicle stimulating hormone (FSH), and luteinizing hormone (LH), the assays were performed with the assistance of the Neodin Veterinary Laboratory, Seoul, South Korea (http://www.vetlab.co.kr). Briefly, serum progesterone concentrations were measured by radioimmunoassay (RIA) using a Coat-a-Count Progesterone Kit (Siemens Medical Solutions Diagnostics, U.S.A.). The kit cont...

show abstract

“…Despite the higher rates of embryonic, fetal, perinatal, and neonatal deaths, as well as offspring with various abnormalities (Edwards et al 2003;Hwang et al 2009), many of the somatic cell clones survived beyond puberty, and they were physiologically, immunologically, and behaviorally normal (Govoni et al 2002;Savage et al 2003). However, due to certain errors such as epigenetic reprogramming or physiological abnormality (Kang et al 2001;Jaenisch and Bird 2003;, food products Note: ALT ¼ alanine aminotransaminase; AST ¼ aspartate aminotransferase; ASP ¼ acylation stimulating protein;CK ¼ creatine kinase; BUN = blood urea nitrogen; T-Bili ¼ total bilirubin; T-Chol ¼ total cholesterol; TG ¼ triglycerladehyde; TP ¼ total protein; A/G ratio ¼ Albumin/Globulin ratio; LDH ¼ lactate dehydrogenase.…”

Section: Discussionmentioning

confidence: 99%

No Long-term Feeding Toxicities on the Health Status in Rats Fed with Cloned Korean Native Beef Cattle (Hanwoo) Meat

Lee

Yang

et al. 2012

Toxicol Pathol

View full text Add to dashboard Cite

This study was designed to undertake a risk assessment to identify the health status of rats fed with somatic cell nuclear transfer (SCNT)-cloned Korean native beef cattle (Hanwoo) meat for 26 weeks. The rats were randomly divided into 5 groups, each consisting of 12 male (142.6 + 5.23 g) and 12 female (113.7 + 6.31 g) rats each. The animals were fed commercial pellets (control), pellets containing 5% (N-5) and 10% (N-10) of normal cattle meat, and diets containing 5% (C-5) and 10% (C-10) of cloned cattle meat. The mortality; clinical signs; body weight; food consumption; urinary, hematology, blood biochemistry, and histopathological analyses; and absolute and relative organ weights were analyzed and compared. During the 26-week test period, health status-related factors of the rats fed on cloned Hanwoo meat were found to have no test substancerelated toxicities. The only difference was the increased uterus weight in female C-10 rats as compared to their counterparts counterparts (p < .05). On the basis of these health status results, it can be postulated that no food consumption risks might arise from the long-term feeding of cloned cattle meat in rats.

show abstract

Age-Related Changes of the Somatotropic Axis in Cloned Holstein Calves1

Cited by 38 publications

References 38 publications

First lactation ovarian function in dairy heifers in relation to prepubertal metabolic profiles

First lactation ovarian function in dairy heifers in relation to prepubertal metabolic profiles

Serum Levels of Reproductive Hormones and Ultrasonographic Monitoring of Ovarian Follicles in Female Cloned Dogs

No Long-term Feeding Toxicities on the Health Status in Rats Fed with Cloned Korean Native Beef Cattle (Hanwoo) Meat

Contact Info

Product

Resources

About