Age-related decrease in aromatase and estrogen receptor (ERα and ERβ) expression in rat testes: protective effect of low caloric diets

Hamden, Khaled; Silandre, Dorothèe; Delalande, Christelle; Feki, Amal; Carreau, Serge

doi:10.1111/j.1745-7262.2008.00343.x

Cited by 17 publications

(14 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Interestingly, our results suggest that ESR1 protein expression was reduced during aging but that ESR2 expression was unaltered. Our results for ESR1 are consistent with previous observations that ESR1 mRNA in the testis is reduced during aging [Hamden et al 2008], but to our knowledge, we are the first to report a change in protein expression in rats. Decreases in ESR1 expression during aging have also been reported in other tissues such as the kidney [Pereira-Simon et al 2012; Sharma and Thakur 2004].…”

Section: Discussionsupporting

confidence: 95%

Alterations in the estrogen environment of the testis contribute to declining sperm production in aging rats

Clarke

Pearl

2014

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

Testicular function, specifically, production of testosterone by Leydig cells, diminishes during aging. Estradiol is also produced by the testis and potentially acts in an autocrine or paracrine manner to help regulate spermatogenesis. However, changes in estradiol concentration or receptor expression within the testis during aging remain unclear. This study was designed to test the hypothesis that the estrogen environment of the testis is altered during aging and that these changes are associated with declining sperm production. Sprague Dawley rats were examined at three, 15, 18, and 21 months of age to detail changes in sperm production and testicular concentration of testosterone and estradiol; five rats were used at three and 21 months and three rats were used at 15 and 18 months. Daily sperm production declined 49% from 15 to 21 months of age. Testicular concentrations of estradiol declined 53% from 15 to 21 months of age; testosterone concentrations were not significantly different. These results suggest that declines in intra-testicular estradiol may contribute to declining sperm production. We further tested our hypothesis by treating rats once every third day with a subcutaneous injection of estradiol valerate (1 µg/kg) from 15 to 18 months of age. Estradiol was increased 54% in treated animals while testosterone was unaffected. Estrogen receptor alpha (ESR1) expression was significantly reduced from 15 to 18 months and expression in estrogen-treated animals was significantly higher than age-matched controls. Additionally, ESR1 expression in 18 month treated animals was not different from 15 months of age. Importantly, daily sperm production in 18 month treated animals was 22% higher than age-matched controls; thus, treatment prevented approximately half of the decline observed in control animals. Collectively, our results suggest that estrogen is involved in maintaining optimum spermatogenesis in adult rats and that estrogen treatment may attenuate the age-associated loss in sperm production.

show abstract

Section: Discussionsupporting

confidence: 95%

Alterations in the estrogen environment of the testis contribute to declining sperm production in aging rats

Clarke

Pearl

2014

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

show abstract

“…The HFCR animals gained less body weight, accumulated lower body fat, hepatic triglyceride, and cholesterol than HF animals, which is a similar pattern of previous studies [21, 28]. HFCR decreased the levels of lipid peroxidation demonstrated by MDA, a generally accepted biomarkers of lipid peroxidation [29].…”

Section: Discussionsupporting

confidence: 86%

Calorie Restriction with a High-Fat Diet Effectively Attenuated Inflammatory Response and Oxidative Stress-Related Markers in Obese Tissues of the High Diet Fed Rats

Park

Valacchi

et al. 2012

Mediators of Inflammation

View full text Add to dashboard Cite

Obesity characterized by increased mass of adipose tissue leads to systemic inflammation. Calorie restriction (CR) improves parameters associated with immune response and antioxidant defense. We hypothesized that CR with a high fat diet (HFCR) regulates local and systemic inflammation and oxidative stress damage in a high fat diet induced obesity (HF group). We investigated effect of HFCR on inflammation and oxidative stress-related markers in liver and adipose tissues as well as adipokines in plasma. HFCR lowered liver triglyceride levels, total cholesterol levels, and the plasma leptin/adiponectin ratio to normal levels and improved glucose tolerance. HFCR also improved fatty liver and normalized adipocyte size and morphology. HFCR reduced lipid peroxidation and decreased the expression levels of inducible nitric oxide synthetase, cyclooxygenase-2, NF-E2-related factor, and heme oxygenase-1 in the liver. Moreover, HFCR suppressed the expression levels of C- reactive protein and manganese superoxide dismutase in the adipose tissue in the HF group. These results suggest that HFCR may have beneficial effects on inflammation and oxidative stress as well as lipid profiles in the HF diet induced obesity. Moreover, HFCR may be a good way to increase compliance in obese patients and to prevent obesity induced complications without changes in dietary pattern.

show abstract

“…All these changes can alter the testicular cells, including spermatozoa and, therefore, the sperm production leading to an alteration of the male fertility. Although we did not perform quantitative analyses of the germ cells, it was clear that the spermatogenesis was altered in the untreated 18-monthold rat testes, which likely accounts for the decrease in testicular weight reported by Henkel et al [36] and in the agreement with our previous study in which a diminution of the number of spermatozoa has been observed in aged rats [37].…”

Section: Tel: +86-21-5492-2824; Fax: +86-21-5492-2825; Shanghai Chinasupporting

confidence: 89%

“…However, estrogens enhance the antioxidant enzyme activities, such as SOD, CAT and GPx [12] and, therefore, could protect the testicular cells against damage and death produced by free radicals. Moreover, this suggests that the beneficial effect of a caloric restriction diet could be in part mediated by estrogens via a parallel increase of ERs expression, as reported elsewhere [37]. Indeed, lower estrogen levels are responsible for enhanced-free radical generation [40,41], leading to an increase in lipid peroxidation, and a concommittant reduction of the antioxidant barrier activity, especially in the testis [12].…”

Section: Tel: +86-21-5492-2824; Fax: +86-21-5492-2825; Shanghai Chinasupporting

confidence: 58%

Protective effects of estrogens and caloric restriction during aging on various rat testis parameters

Hamden¹,

Silandre²,

Delalande³

et al. 2008

Asian Journal of Andrology

Self Cite

View full text Add to dashboard Cite

show abstract

Age-related decrease in aromatase and estrogen receptor (ERα and ERβ) expression in rat testes: protective effect of low caloric diets

Abstract: These results indicate that during aging, a low caloric diet (not undernutrition) is beneficial for spermatogenesis and likely improves the protection of the cells via an increase of the cellular antioxidant defense system in which aromatase/ER could play a role.

Cited by 17 publications

References 27 publications

Alterations in the estrogen environment of the testis contribute to declining sperm production in aging rats

Alterations in the estrogen environment of the testis contribute to declining sperm production in aging rats

Calorie Restriction with a High-Fat Diet Effectively Attenuated Inflammatory Response and Oxidative Stress-Related Markers in Obese Tissues of the High Diet Fed Rats

Protective effects of estrogens and caloric restriction during aging on various rat testis parameters

Contact Info

Product

Resources

About