Age-Related Differences in Percentages of Regulatory and Effector T Lymphocytes and Their Subsets in Healthy Individuals and Characteristic STAT1/STAT5 Signalling Response in Helper T Lymphocytes

Holcar, Marija; Goropevšek, Aleš; Ihan, Alojz; Avčin, Tadej

doi:10.1155/2015/352934

Cited by 31 publications

(19 citation statements)

References 55 publications

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“…In agreement with the literature for the frequency of total circulating Tregs in peripheral blood of control children , Tregs were found at a frequency of 5.8% ± 1.9 in the control group; the frequency of Tregs in cases was significantly lower (4.0% ± 1.3, p ≤ 0.001) (Fig. A).…”

Section: Resultssupporting

confidence: 91%

T‐cell responses against rhinovirus species A and C in asthmatic and healthy children

Gaido

Granland

Laing

et al. 2017

Immunity Inflam & Disease

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BackgroundInfections by rhinovirus (RV) species A and C are the most common causes of exacerbations of asthma and a major cause of exacerbations of other acute and chronic respiratory diseases. Infections by both species are prevalent in pre‐school and school‐aged children and, particularly for RV‐C, can cause severe symptoms and a need for hospitalization. While associations between RV infection and asthma are well established, the adaptive immune‐mechanisms by which RV infections influence asthma exacerbations are yet to be defined.ObjectiveThe aim of this study was to characterize and compare T‐cell responses between RV‐A and RV‐C and to test the hypothesis that T‐cell responses would differ between asthmatic children and healthy controls.MethodsA multi‐parameter flow cytometry assay was used to characterize the in vitro recall T‐cell response against RV‐A and RV‐C in PBMCs from children with acute asthma (n = 22) and controls (n = 26). The responses were induced by pools of peptides containing species‐specific VP1 epitopes of RV‐A and RV‐C.ResultsRegardless of children's clinical status, all children that responded to the in vitro stimulation (>90%) had a similar magnitude of CD4+ T‐cell responses to RV‐A and RV‐C. However, asthmatic children had a significantly lower number of circulating regulatory T cells (Tregs), and healthy controls had significantly more Tregs induced by RV‐A than RV‐C.Conclusions and Clinical RelevanceThe comparable recall memory T‐cell responses in asthmatic and control children to both RV‐A and RV‐C show that differences in the antibody and inflammatory responses previously described are likely to be due to regulation, with a demonstrated candidate being reduced regulatory T‐cells. The reduced Treg numbers demonstrated here could explain the asthmatic's inability to appropriately control immunopathological responses to RV infections.

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Section: Resultssupporting

confidence: 91%

T‐cell responses against rhinovirus species A and C in asthmatic and healthy children

Gaido

Granland

Laing

et al. 2017

Immunity Inflam & Disease

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“…Interestingly, age at the time of primary EBV infection was also significantly younger in CHL groups while the timings of primary EBV infection after Tx were not different between groups. Given the inefficient development of effector T cell in younger age, it is plausible EBV infection in younger age by itself is an additional risk factor for CHL carrier state . The kinetics of BKV and CMV were analyzed by EBV viral load group with the assumption that the intensity of immunosuppression is a key factor promoting the development of uncontrolled chronic viral infection after KTx .…”

Section: Discussionmentioning

confidence: 99%

Epidemiology and outcome of chronic high Epstein‐Barr viral load carriage in pediatric kidney transplant recipients

Yamada

Nguyen

Fadakar

et al. 2018

Pediatric Transplantation

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The development of EBV infection and PTLD is normally associated with a high EBV viral load in peripheral blood. Observations have previously identified existence of a CHL carrier state that demonstrated variable outcomes based upon the organ which was transplanted. Data defining the incidence and outcome of CHL in pediatric KTx are not well described. The charts of children undergoing isolated KTx at Children's Hospital of Pittsburgh between January 2000 and December 2014 were retrospectively reviewed. EBV loads in the peripheral blood were routinely measured as part of surveillance protocols at our center. CHL was defined as the presence of high load for >50% of samples for ≥6 months. PTLD was defined histologically using WHO definitions. Of 188 isolated KTx recipients, we identified a total of 16 (8%) children who developed CHL carrier state. No patient developed EBV-driven late-onset PTLD. Age at the time of KTx was significantly lower in the CHL group (median 3.9 years, interquartile range: IQR 2.9-6.6, P = .0004). Children in the CHL group were more likely to be EBV-seronegative prior to KTx (94%, 15/16), compared to the UVL and LVL groups (55% and 50%, respectively, P < .002). The median duration of CHL carrier state was 20 months (IQR 10.7-35.8). Fifteen of the 16 CHL carriers experienced spontaneous resolution of CHL carrier state. Children in the CHL group were younger at the time of primary EBV infection (P = .023). Finally, antiviral medication was not effective in either preventing or decreasing the EBV viral load in blood (P = .84). Overall incidence of late-onset PTLD is very low compared to heart and intestinal transplant, even though KTx recipients can develop CHL carrier state. The CHL carriers in KTx recipients were EBV-seronegative prior to transplant and were younger both at the time of KTx and at the time of primary EBV infection compared to those in the UVL and HVL groups. Antivirals did not prevent EBV infection or decrease EBV viral loads.

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“…1). These cells are involved in the development of tissue inflammation, are less susceptible to suppression by FoxP3+ Treg-lymphocytes and are present in rather high numbers in healthy adult persons [39,40]. Both Th17-and Th1/Th17-lymphocytes are characterized by high plasticity and can be polarized into different T-helper subpopulations if proper micro-environmental milieu is created [25,39,41].…”

Section: Discussionmentioning

confidence: 99%

T-helper-1, T-helper-17, T-regulatory lymphocytes in hypertensive patients with diabetes mellitus type 2 or impaired glucose tolerance: association with clinical and metabolic parameters in a case control study

et al. 2016

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Age-Related Differences in Percentages of Regulatory and Effector T Lymphocytes and Their Subsets in Healthy Individuals and Characteristic STAT1/STAT5 Signalling Response in Helper T Lymphocytes

Cited by 31 publications

References 55 publications

T‐cell responses against rhinovirus species A and C in asthmatic and healthy children

T‐cell responses against rhinovirus species A and C in asthmatic and healthy children

Epidemiology and outcome of chronic high Epstein‐Barr viral load carriage in pediatric kidney transplant recipients

T-helper-1, T-helper-17, T-regulatory lymphocytes in hypertensive patients with diabetes mellitus type 2 or impaired glucose tolerance: association with clinical and metabolic parameters in a case control study

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