Age-Related Downregulation of CCN2 Is Regulated by Cell Size in a YAP/TAZ-Dependent Manner in Human Dermal Fibroblasts: Impact on Dermal Aging

Qin, Zhaoping; Torebjörk, H. E.; Guo, Chunfang; Quan, Taihao

doi:10.1016/j.xjidi.2022.100111

Cited by 13 publications

(31 citation statements)

References 59 publications

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“…The co-cultured scaffolds showed an average of ∼140

20 cells (both HaCaT and HDFa) in a visualized area of 581.2 × 581.2 μ m, consisting of 88

16 HaCaT cells and 52

4 HDFa cells. This results in 13000

1500 cells in the well (culturable surface area of 0.32 × 10 8 μ m 2 ; n = 2, two biological replicates with two fields of view per well, error represents SEM), which based on the average cell area for HaCaT and HDFa cells (3000 μ m 2 and 4000 μ m 2 ) respectively [ [76] , [77] , [78] , [79] ], corresponds to a coverage of close to 100% of the well surface [ 80 , 81 ].…”

Section: Resultsmentioning

confidence: 99%

Adding a new dimension: Multi-level structure and organization of mixed-species Pseudomonas aeruginosa and Staphylococcus aureus biofilms in a 4-D wound microenvironment

Dhekane

Mhade²,

Kaushik

2022

Biofilm

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“…The co-cultured scaffolds showed an average of ∼140

20 cells (both HaCaT and HDFa) in a visualized area of 581.2 × 581.2 μ m, consisting of 88

16 HaCaT cells and 52

4 HDFa cells. This results in 13000

Section: Resultsmentioning

confidence: 99%

Adding a new dimension: Multi-level structure and organization of mixed-species Pseudomonas aeruginosa and Staphylococcus aureus biofilms in a 4-D wound microenvironment

Dhekane

Mhade²,

Kaushik

2022

Biofilm

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“…The co-cultured scaffolds showed an average of 140+16 cells (both HaCaT and HDFa) in a visualized area of 581.2 X 581.2 μ m, consisting of 88±16 HaCaT cells and 52±4 HDFa cells. This results in 13000±1500 cells in the well (culturable surface area of 0.32 X 10 8 μ m 2 ; n=2, two biological replicates with two fields of view per well, error represents SEM), which based on the average cell area for HaCaT and HDFa cells, 3000 μ m 2 [54–57] and 4000 μ m 2 [54–57] respectively, corresponds to a coverage of close to 100% of the well surface [58,59]. It is important to note that in the composite 4-D wound microenvironment, the IVWM would provide matrix elements (collagen, fibrinogen, fibronectin), which would be present between, and in close association, with the host cells.…”

Section: Resultsmentioning

confidence: 99%

Adding a new dimension: Multi-level structure and organization of mixed-species Pseudomonas aeruginosa and Staphylococcus aureus biofilms in a 4-D wound microenvironment

Dhekane

Mhade²,

Kaushik

2022

Preprint

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Biofilms in wounds typically consist of aggregates of bacteria, most often Pseudomonas aeruginosa and Staphylococcus aureus, in close association with each other and the host microenvironment. Given this, the interplay across host and microbial elements, including the biochemical and nutrient profile of the microenvironment, likely influences the structure and organization of wound biofilms. While clinical studies, in vivo and ex vivo model systems have provided insights into the distribution of P. aeruginosa and S. aureus in wounds, they are limited in their ability to provide a detailed characterization of biofilm structure and organization across the host-microbial interface. On the other hand, biomimetic in vitro systems, such as host cell surfaces and simulant media conditions, albeit reductionist, have been shown to support the co-existence of P. aeruginosa and S. aureus biofilms, with species-dependent localization patterns and interspecies interactions. Therefore, composite in vitro models that bring together key features of the wound microenvironment could provide unprecedented insights into the structure and organization of mixed-species biofilms. We have built a four-dimensional (4-D) wound microenvironment consisting of a 3-D host cell scaffold of co-cultured human epidermal keratinocytes and dermal fibroblasts, and an in vitro wound milieu (IVWM); the IVWM provides the fourth dimension that represents the biochemical and nutrient profile of the wound infection state. We leveraged this composite 4-D wound microenvironment to probe the structure of mixed-species P. aeruginosa and S. aureus biofilms across multiple levels of organization such as aggregate dimensions and biomass thickness, species co-localization and organization within the biomass, overall biomass composition and interspecies interactions. In doing so, the composite 4-D wound microenvironment platform provides multi-level insights into the structure of mixed-species biofilms, which we incorporate into the current understanding of P. aeruginosa and S. aureus organization in the wound bed.

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“…Matricellular proteins such as the CCN family can bind to TGFβ, Notch, and the Wnt co-receptor LRP6, thus affecting signaling outcomes [ 148 , 219 , 220 , 221 ], although their roles in sebaceous glands aging have not been reported. In line with this, CCN protein expressions are changed during aging, with CCN1 upregulated and CCN2/CTGF (connective tissue growth factor) downregulated in the dermis of aged human skin [ 169 , 170 , 171 ].…”

Section: Matricellular Proteins In Stem Cell Regulation During Homeos...mentioning

confidence: 93%

Matricellular Proteins in the Homeostasis, Regeneration, and Aging of Skin

Raja,

Clarin,

Yanagisawa

2023

IJMS

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Matricellular proteins are secreted extracellular proteins that bear no primary structural functions but play crucial roles in tissue remodeling during development, homeostasis, and aging. Despite their low expression after birth, matricellular proteins within skin compartments support the structural function of many extracellular matrix proteins, such as collagens. In this review, we summarize the function of matricellular proteins in skin stem cell niches that influence stem cells’ fate and self-renewal ability. In the epidermal stem cell niche, fibulin 7 promotes epidermal stem cells’ heterogeneity and fitness into old age, and the transforming growth factor-β—induced protein ig-h3 (TGFBI)—enhances epidermal stem cell growth and wound healing. In the hair follicle stem cell niche, matricellular proteins such as periostin, tenascin C, SPARC, fibulin 1, CCN2, and R-Spondin 2 and 3 modulate stem cell activity during the hair cycle and may stabilize arrector pili muscle attachment to the hair follicle during piloerections (goosebumps). In skin wound healing, matricellular proteins are upregulated, and their functions have been examined in various gain-and-loss-of-function studies. However, much remains unknown concerning whether these proteins modulate skin stem cell behavior, plasticity, or cell–cell communications during wound healing and aging, leaving a new avenue for future studies.

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Age-Related Downregulation of CCN2 Is Regulated by Cell Size in a YAP/TAZ-Dependent Manner in Human Dermal Fibroblasts: Impact on Dermal Aging

Cited by 13 publications

References 59 publications

Adding a new dimension: Multi-level structure and organization of mixed-species Pseudomonas aeruginosa and Staphylococcus aureus biofilms in a 4-D wound microenvironment

Adding a new dimension: Multi-level structure and organization of mixed-species Pseudomonas aeruginosa and Staphylococcus aureus biofilms in a 4-D wound microenvironment

Adding a new dimension: Multi-level structure and organization of mixed-species Pseudomonas aeruginosa and Staphylococcus aureus biofilms in a 4-D wound microenvironment

Matricellular Proteins in the Homeostasis, Regeneration, and Aging of Skin

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