Age-related features in expression of calcium-binding proteins in autonomic ganglionic neurons

Maslyukov, P. M.; Nozdrachev, A. D.; Emanuilov, A. I.

doi:10.1134/s207905701604010x

Cited by 4 publications

(1 citation statement)

References 61 publications

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“…CB and CR are found in separate groups of neurons in the central and peripheral nervous systems (Hof et al, 1999; Masliukov, Nozdrachev, & Emanuilov, 2016; Schmidt, 2012; Schwaller, 2014). In the hypothalamus, CB and CR are observed in the medial preoptic area, paraventricular, suprachiasmatic, supraoptic, infundibular, arcuate, ventromedial, dorsomedial nucleus, lateral hypothalamus and the mammillary bodies (Belekhova, Kenigfest, Chernigovskaya, & Veselkin, 2017; Bogus‐Nowakowska, 2019; Celio, 1990; Eghlidi et al, 2018; Foo, Hellysaz, & Broberger, 2014; Fortin & Parent, 1997; Montelli, Graïc, Ruiz, Stelletta, & Peruffo, 2020).…”

Section: Introductionmentioning

confidence: 99%

Expression of calbindin and calretinin in the dorsomedial and ventromedial hypothalamic nuclei during aging

Vishnyakova

Moiseev

Spirichev

et al. 2020

The Anatomical Record

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The hypothalamus is involved in the regulation of rhythms, autonomic, endocrine, and behavioral functions and may also participate in aging development and control. The aim of this work was to study the expression of calbindin (CB) and calretinin (CR) in the ventromedial (VMH) and dorsomedial (DMH) hypothalamic nuclei in young and old rats of both sexes by immunohistochemistry and western blotting. In young animals, the largest number of CB‐immunoreactive (IR) neurons was detected in the ventral part of DMH (DMHv) and smaller percentage was found in its dorsal part (DMHd), in the dorsomedial part of the VMH (VMHdm) and in the ventrolateral part of the VMH (VMHvl). In aged animals, the percentage of CB‐IR neurons significantly decreased in all studied nuclei, including DMHv, DMHd, VMHdm and VMHvl. CR‐IR neurons were found in moderate number in the DMHv, DMHd, VMHdm and VMHvl of young rats. In aged rats, the percentage of CR‐IR neurons significantly increased in the DMHv, DMHd, VMHdm and VMHvl. Less than one third of IR neurons colocalized CB and CR in young and aged rats. The expression of CB significantly decreased, and the expression of CR significantly increased in the DMH and VMH during aging by western blot analysis. Thus, there are opposite changes of the calcium‐binding proteins expression in the hypothalamic nuclei involved in the metabolic and sexual regulation during aging.

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Section: Introductionmentioning

confidence: 99%

Expression of calbindin and calretinin in the dorsomedial and ventromedial hypothalamic nuclei during aging

Vishnyakova

Moiseev

Spirichev

et al. 2020

The Anatomical Record

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Development of Calbindin- and Calretinin-Immunopositive Neurons in the Enteric Ganglia of Rats

et al. 2016

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Calbindin D28 K (CB) and calretinin (CR) are the members of the EF-hand family of calcium-binding proteins that are expressed in neurons and nerve fibers of the enteric nervous system. CB and CR are expressed differentially in neuronal subpopulations throughout the central and peripheral nervous systems and their expression has been used to selectively target specific cell types and isolate neuronal networks. The present study presents an immunohistochemical analysis of CB and CR in the enteric ganglia of small intestine in rats of different ages (newborn, 10-day-old, 20-day-old, 30-day-old, 60-day-old, 1-year-old, and 2-year-old). The data obtained suggest a number of age-dependent changes in CB and CR expression in the myenteric and submucous plexuses. In the myenteric plexus, the lowest percentage of CB-immunoreactive (IR) and CR-IR neurons was observed at birth, after which the number of IR cells increased in the first 10 days of life. In the submucous plexus, CB-IR and CR-IR neurons were observed from 10-day-old onwards. The percentage of CR-IR and CB-IR neurons increased in the first 2 months and in the first 20 days, respectively. In all animals, the majority of the IR neurons colocalized CR and CB. From the moment of birth, the mean of the cross-sectional area of the CB-IR and CR-IR neuronal profiles was larger than that of CB- and CR-negative cells.

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Neurochemical Features of Metasympathetic System Ganglia in the Course of Ontogenesis

2017

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Age-related features in expression of calcium-binding proteins in autonomic ganglionic neurons

Cited by 4 publications

References 61 publications

Expression of calbindin and calretinin in the dorsomedial and ventromedial hypothalamic nuclei during aging

Expression of calbindin and calretinin in the dorsomedial and ventromedial hypothalamic nuclei during aging

Development of Calbindin- and Calretinin-Immunopositive Neurons in the Enteric Ganglia of Rats

Neurochemical Features of Metasympathetic System Ganglia in the Course of Ontogenesis

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