Age-related impaired Th1 responses to PRV vaccine in vivo in aged pigs

Ma, Miaopeng; Wang, Lingling; Yang, Jun; Cai, Hao; Shi, Juqing; Zhang, Shuiqin; Huang, Zhifeng; Zhang, Linghua

doi:10.1016/j.molimm.2012.05.016

Cited by 6 publications

(9 citation statements)

References 36 publications

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“…This finding was consistent with our previous studies reporting that aged pigs had a decreased capacity to respond effectively with protein antigens [4]. However, when young and aged animals were immunized with a PRV vaccine formulation containing CpG ODN, the levels of anti-PRV antibodies present in collected serum samples were increased by approximately 3-fold in the young pigs (at day 14) and by 3-fold (at day 14) in aged mice, over the levels of antibodies present in the serum of young or aged pigs immunized with PRV alone (Table 2).…”

Section: Resultssupporting

confidence: 94%

“…Landrace×Yorkshire×Durok Pigs (Swine Seed Breeding Center of Guangzhou) of different ages were used in this study, and 5.5 year-old pigs were seen as aged pigs 4,18. The conventional pigs were kept under standard farming conditions.…”

Section: Methodsmentioning

confidence: 99%

“…Pseudorabies virus, the causative agent of Aujeszky’s disease, is a neurotropic alphaherpesvirus that produces fatal encephalitis in newborn pigs and a milder syndrome in older animals [15]. We [4], [12], [14] had reported good immune responses induced by a attenuated pseudorabies virus (PRV) vaccine in seronegative young and aged pigs, the aim of this study was therefore to evaluate the effectiveness of PRV vaccine/CpG-ODN intramuscular (IM) administration to induce an PRV-specific immune responses in aged pigs. Herein we showed that CpG ODN formulated with PRV vaccine could restore the impaired Th1 responses in aged pigs, which were associated with enhanced PRV-specific CD4+ T cells priming.…”

Section: Introductionmentioning

confidence: 99%

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Immunization of Aged Pigs with Attenuated Pseudorabies Virus Vaccine Combined with CpG Oligodeoxynucleotide Restores Defective Th1 Immune Responses

et al. 2013

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Background and AimsAttempts to immunize aged subjects often result in the failure to elicit a protective immune response. Murine model studies have shown that oligonucleotides containing CpG motifs (CpG ODN) can stimulate immune system in aged mice as effectively as in young mice. Since many physiological and pathophysiological data of pigs can be transferred to humans, research in pigs is important to confirm murine data. Here we investigated whether immunization of aged pig model with attenuated pseudorabies virus vaccine (PRV vaccine) formulated with CpG ODN could promote a successful development of immune responses that were comparable to those induced in young pigs in a similar manner.MethodologyYoung and aged pigs were immunized IM with PRV vaccine alone, or in combination with CpG ODN respectively. At days 3, 7, 14 post immunization sera were assayed by ELISA for IgG titres, at day 7 for IgG1 and IgG2 subtypes titres. All blood samples collected in evacuated test tubes with K-EDTA at day 7 were analyzed for flow cytometer assay. Blood samples at day 7 collected in evacuated test tubes with heparin were analysed for antigen-specific cytokines production and peripheral blood mononuclear cells (PBMCs) proliferative responses.ResultsCpG ODN could enhance Th1 responses (PRV-specific IgG2/IgG1 ratio, proliferative responses, Th1 cytokines production) when used as an adjuvant for the vaccination of aged pigs, which were correlated with enhanced CD4+ T cells percentage, decreased CD4+CD8+CD45RO+ T cells percentage and improved PRV-specific CD4+ T cells activation.ConclusionsOur results demonstrate a utility for CpG ODN, as a safe vaccine adjuvant for promoting effective systemic immune responses in aged pig model. This agent could have important clinical uses in overcoming some of age-associated depressions in immune function that occur in response to vaccination.

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Section: Resultssupporting

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immunization of Aged Pigs with Attenuated Pseudorabies Virus Vaccine Combined with CpG Oligodeoxynucleotide Restores Defective Th1 Immune Responses

et al. 2013

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“…Interestingly, CQ treatment during chronic SIV infection demonstrated a reduction of immune activation and a better recovery of CD4 + T cells but this did not affect virus levels. 22 Although our study was limited by the small number of control animals, the data suggest that treatment with CQ during the acute onset of infection may not sufficiently reduce chronic immune activation to have a beneficial effect in the long term.…”

mentioning

confidence: 86%

“…3,[19][20][21] While in vivo studies in macaques chronically infected with SIV mac239 confirmed a reduction in pDC activation following CQ administration, no effects were seen in the viral load or cellular composition in the treated animals. 22 Because HIV activates pDC through TLR, including TLR7, 19,21 CQ may interfere with endosomal maturation of TLR7 and with the HIV effect on TLR7 signaling. 23 In the clinical setting, treatment with hydroxychloroquine (HCQ) or with chloroquine of antiretroviral-treated (ART) 24 as well as ART-naive patients 25 resulted in the reduction of immune activation but had little or no effect on CD4 + T cell recovery and viral load reduction, possibly due to the limited number of subjects enrolled in these studies.…”

mentioning

confidence: 99%

Transient Increase of Interferon-Stimulated Genes and No Clinical Benefit by Chloroquine Treatment During Acute Simian Immunodeficiency Virus Infection of Macaques

Vaccari

Fenizia

et al. 2014

AIDS Research and Human Retroviruses

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Simian immunodeficiency virus (SIV) infection leads to AIDS in experimentally infected Rhesus macaques similarly to HIV-infected humans. In contrast, SIV infection of natural hosts is characterized by a down-regulation of innate acute responses to the virus within a few weeks of infection and results in limited pathology. Chloroquine (CQ) has been used in the treatment or prevention of malaria and has recently been shown to cause a decrease of immune activation and CD4 cell loss in HIV-infected individuals treated with antiretroviral therapy. Here, we treated Rhesus macaques with CQ during the acute phase of SIV mac251 infection with the intent to decrease viral-induced immune activation and possibly limit disease progression. Contrary to what was expected, CQ treatment resulted in a temporary increased expression of interferon (IFN)-stimulating genes and it worsened the recovery of CD4 + T cells in the blood. Our findings confirm recent results observed in asymptomatic HIVinfected patients and suggest that CQ does not provide an obvious benefit in the absence of antiretroviral therapy.

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Oil-based adjuvants delivered intradermally induce high primary IgG2 immune response in swine

Oreskovic

Kudláčková

Krejcí

et al. 2017

Research in Veterinary Science

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Age-related impaired Th1 responses to PRV vaccine in vivo in aged pigs

Cited by 6 publications

References 36 publications

Immunization of Aged Pigs with Attenuated Pseudorabies Virus Vaccine Combined with CpG Oligodeoxynucleotide Restores Defective Th1 Immune Responses

Immunization of Aged Pigs with Attenuated Pseudorabies Virus Vaccine Combined with CpG Oligodeoxynucleotide Restores Defective Th1 Immune Responses

Transient Increase of Interferon-Stimulated Genes and No Clinical Benefit by Chloroquine Treatment During Acute Simian Immunodeficiency Virus Infection of Macaques

Oil-based adjuvants delivered intradermally induce high primary IgG2 immune response in swine

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