Age-related loss of DNA repair synthesis in isolated rat myocardial cells

Lampidis, T. J.; Schaiberger, George E.

doi:10.1016/0014-4827(75)90276-1

Cited by 47 publications

(9 citation statements)

References 11 publications

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“…2) might possibly be due to a decrease in DNA repair. DNA repair has been reported to decline with age in isolated (47). Whether this, in turn, is due to DNA or protein damage is unclear.…”

Section: Discussionmentioning

confidence: 99%

7-Methylguanine adducts in DNA are normally present at high levels and increase on aging: analysis by HPLC with electrochemical detection.

Park

Ames

1988

Proc. Natl. Acad. Sci. U.S.A.

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“…2) might possibly be due to a decrease in DNA repair. DNA repair has been reported to decline with age in isolated (47). Whether this, in turn, is due to DNA or protein damage is unclear.…”

Section: Discussionmentioning

confidence: 99%

7-Methylguanine adducts in DNA are normally present at high levels and increase on aging: analysis by HPLC with electrochemical detection.

Park

Ames

1988

Proc. Natl. Acad. Sci. U.S.A.

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“…Alexander [8] was the first to speculate that DNA repair is turned off during mammalian development as cells differentiate to the postmitotic state. There is evidence that the DNA repair capacity of muscle cells is lower in postmitotic cells than in proliferating cells from embryonic or newborn rats [9,10]. However, these studies have used a variety of DNA damaging agents and these agents give different results.…”

Section: Introductionmentioning

confidence: 99%

Elevated DNA Excision Repair Capacity in the Extraembryonic Mesoderm of the Midgestation Mouse Embryo

Latimer

Hultner

Cleaver

et al. 1996

Experimental Cell Research

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In order to determine whether there is differential cell-type-specific DNA repair we measured the nucleotide excision repair capacity of the four distinct cell lineages that comprise the extraembryonic yolk sac using the unscheduled DNA synthesis assay. Yolk sacs from mouse embryos at 11.5-12.5 days gestation were microdissected to yield purified trophoblast, parietal endoderm, mesoderm, and visceral endoderm, as well as fetal skin fibroblasts which were then grown as primary explants. At this midgestational stage of development, the yolk sac provides essential functions for the sustenance of the embryo while the complex process of organogenesis is proceeding in the liver, kidney, and gut. Trophoblast giant cells, parietal endoderm, and visceral endoderm all demonstrated low levels of unscheduled DNA synthesis consistent with levels measured in adult mouse skin fibroblasts. As has previously been documented, embryonic mouse skin fibroblasts were reproducibly 2-to 3-fold higher than adult mouse skin fibroblasts in levels of DNA excision repair. The extraembryonic mesoderm, however, displayed a statistically significant level of unscheduled DNA synthesis 10-fold higher than adult mouse skin fibroblasts or the other lineages of the midgestation yolk sac. Further, the S-indexes of these lineages were also determined to assess the possible relevance of differential repair to the proliferative status of the cells. These data demonstrate that DNA excision repair capacity is lineage-specific during embryogenesis in the mouse. These studies may begin to provide a context for understanding the perplexing developmental aspects such as the characteristic congenital abnormalities associated with the human heritable DNA repair deficiency diseases.

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“…Primary cultures derived from neonatal rat hearts consist of morphologically distinguishable cardiac muscle cells and nonmuscle cells (epithelial and fibroblastic) . The cardiac muscle cells are nondivid- ing and undergo rapid rhythmic contractions in culture (11). When exposed to potential-dependent probes, cardiac muscle cells show much higher levels of mitochondria-associated fluorescence than do the nonmuscle cells present in the same culture (Fig.…”

Section: Possible Relationship Between Mitochondrial Membrane Potentimentioning

confidence: 99%

“…Observed differences in the intensity of mitochondria-associated fluorescence may reflect differences in the functional state of mitochondria, more specifically, differences in the magnitude of mitochondrial trans-membrane potential . (17) and rat cardiac muscle cells (11) were also examined.…”

mentioning

confidence: 99%

Monitoring of relative mitochondrial membrane potential in living cells by fluorescence microscopy.

Johnson¹,

Walsh²,

Bockus³

et al. 1981

The Journal of Cell Biology

862

415

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Permeant cationic fluorescent probes are shown to be selectively accumulated by the mitochondria of living cells. Mitochondria-specific interaction of such molecules is apparently dependent on the high trans-membrane potential (inside negative) maintained by functional mitochondria. Dissipation of the mitochondrial trans-membrane and potential by ionophores or inhibitors of electron transport eliminates the selective mitochondrial association of these compounds. The application of such potential-dependent probes in conjunction with fluorescence microscopy allows the monitoring of mitochondrial membrane potential in individual living cells. Marked elevations in mitochondria-associated probe fluorescence have been observed in cells engaged in active movement. This approach to the analysis of mitochondrial membrane potential should be of value in future investigations of the control of energy metabolism and energy requirements of specific biological functions at the cellular level.

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Age-related loss of DNA repair synthesis in isolated rat myocardial cells

Cited by 47 publications

References 11 publications

7-Methylguanine adducts in DNA are normally present at high levels and increase on aging: analysis by HPLC with electrochemical detection.

7-Methylguanine adducts in DNA are normally present at high levels and increase on aging: analysis by HPLC with electrochemical detection.

Elevated DNA Excision Repair Capacity in the Extraembryonic Mesoderm of the Midgestation Mouse Embryo

Monitoring of relative mitochondrial membrane potential in living cells by fluorescence microscopy.

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