Age‐related SUMOylation of PLK1 is essential to meiosis progression in mouse oocytes

Mo, Xiao-Long; Liu, Feng; Shan, Meng-Meng; Yao, Bo; Sun, Qiqi; Zou, Yuan-Jing; Zhang, Kun-Huan; Tan, Jun; Sun, Shao‐Chen; Ren, Yan-Ping

doi:10.1002/jcp.30910

Cited by 2 publications

(1 citation statement)

References 41 publications

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“…One contributor to the poor spindle formation in Ube2i Zp3- cre+ maybe be decreased expression and/or activity of polo-like kinase 1 ( Plk1 ). a SUMO target and overexpression of PLK1 SUMOylation site mutants in oocytes causes increases in the number of abnormal spindles ( 21, 45, 46 ). In oocytes, PLK1 coordinates key events during meiotic resumption and progression, including regulation of chromosome architecture and spindle assembly.…”

Section: Discussionmentioning

confidence: 99%

The E2 SUMO-conjugating enzyme UBE2I coordinates the oocyte and zygotic transcriptional programs

Briley

Ahmed

Jiang

et al. 2022

Preprint

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In mammals, meiotically competent oocytes develop cyclically during ovarian folliculogenesis. During folliculogenesis, prophase I arrested oocytes are transcriptionally active, producing and storing transcripts required for their growth and for early stages of embryogenesis prior to the maternal to zygotic transition. Defective oocyte development during folliculogenesis leads to meiotic defects, aneuploidy, follicular atresia, or non-viable embryos. Here we generated a novel oocyte-specific knockout of the SUMO E2 ligase, Ube2i, using Zp3-cre to test its function during folliculogenesis. Ube2i Zp3-cre+ female mice are sterile with oocytes that arrest in meiosis I with defective spindles and chromosome alignment. Fully grown mutant oocytes abnormally maintain transcription but downregulate maternal effect genes and prematurely activate the zygotic transcriptional program. Thus, this work uncovers UBE2i as a novel orchestrator of chromatin and transcriptional regulation in mouse oocytes.

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Section: Discussionmentioning

confidence: 99%

The E2 SUMO-conjugating enzyme UBE2I coordinates the oocyte and zygotic transcriptional programs

Briley

Ahmed

Jiang

et al. 2022

Preprint

View full text Add to dashboard Cite

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Mutation of the SUMOylation site of Aurora-B disrupts spindle formation and chromosome alignment in oocytes

Chen,

Li,

Yao

et al. 2024

Cell Death Discov.

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Aurora-B is a kinase that regulates spindle assembly and kinetochore-microtubule (KT-MT) attachment during mitosis and meiosis. SUMOylation is involved in the oocyte meiosis regulation through promoting spindle assembly and chromosome segregation, but its substrates to support this function is still unknown. It is reported that Aurora-B is SUMOylated in somatic cells, and SUMOylated Aurora-B contributes the process of mitosis. However, whether Aurora-B is SUMOylated in oocytes and how SUMOylation of Aurora-B impacts its function in oocyte meiosis remain poorly understood. In this study, we report that Aurora-B is modified by SUMOylation in mouse oocytes. The results show that Aurora-B colocalized and interacted with SUMO-2/3 in mouse oocytes, confirming that Aurora-B is modified by SUMO-2/3 in this system. Compared with that in young mice, the protein expression of SUMO-2/3 decreased in the oocytes of aged mice, indicating that SUMOylation might be related to mouse aging. Overexpression of Aurora-B SUMOylation site mutants, Aurora-BK207R and Aurora-BK292R, inhibited Aurora-B recruitment and first polar body extrusion, disrupting localization of gamma tubulin, spindle formation and chromosome alignment in oocytes. The results show that it was related to decreased recruitment of p-HDAC6 which induces the high stability of whole spindle microtubules including the microtubules of both correct and wrong KT-MT attachments though increased acetylation of microtubules. Therefore, our results corroborate the notion that Aurora-B activity is regulated by SUMO-2/3 in oocytes, and that SUMOylated Aurora B plays an important role in spindle formation and chromosome alignment.

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Age‐related SUMOylation of PLK1 is essential to meiosis progression in mouse oocytes

Cited by 2 publications

References 41 publications

The E2 SUMO-conjugating enzyme UBE2I coordinates the oocyte and zygotic transcriptional programs

The E2 SUMO-conjugating enzyme UBE2I coordinates the oocyte and zygotic transcriptional programs

Mutation of the SUMOylation site of Aurora-B disrupts spindle formation and chromosome alignment in oocytes

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