To examine the mode of transmission of BK polyomavirus (BKV), urine samples were collected from Japanese-Americans in Los Angeles and from other southern Californians. Subtype I was the main subtype found in samples from both groups. The subtype I subgroup Ib-2, which is predominant in Europe, was the primary subgroup detected in second-generation Japanese-Americans and in southern Californians; however, the Ic subgroup prevalent in native Japanese was rare in these populations. Since the European subgroup (Ib-2) predominated in the studied geographic area, the findings demonstrate that transmission outside the family is common in the spread of BKV, unlike previous findings for JC polyomavirus.BK polyomavirus (BKV) is ubiquitous in human populations, infecting children asymptomatically and then persisting in the kidneys (11). Renal BKV in immunocompetent individuals is not latent but replicates frequently, and progeny viruses are excreted in urine (27). In immunosuppressed patients (particularly renal transplant recipients), BKV may cause renal dysfunction such as BKV-associated nephropathy (6).BKV isolates worldwide are classified into four subtypes (I to IV) by serological and genotyping methods (11). Subtype I (the most prevalent subtype) is further divided into four subgroups (Ia, Ib-1, Ib-2, and Ic) based on DNA sequence variations (7,19,25). Each of these subgroups has a unique geographic distribution pattern: Ia is prevalent in Africa, Ib-1 in Southeast Asia, Ib-2 in Europe, and Ic in Northeast Asia, including Japan (7,19,25). Similar to JC polyomavirus (JCV), a human polyomavirus closely related to BKV (23), it appears that subtype I BKV evolved in association with the division of human populations. However, recent studies (25, 26) have also revealed certain unique aspects of the relationship between BKV and humans, and further understanding of this relationship requires more information about BKV, including its mode of transmission.The mode of JCV transmission has been studied using urinary JCV DNA, and it has been established that JCV is usually transmitted from parents to children during long-term cohabitation (8,12,18,24). Since BKV is also frequently detected in the urine of immunocompetent individuals (27), the methods used to clarify the mode of JCV transmission should be applicable to BKV. To test the hypothesis that, similar to JCV, BKV is transmitted within the family, here we used urine samples previously collected from Japanese-Americans for the study of JCV transmission (18).Urine samples were collected from Japanese-Americans (second and third generations) and from other southern Californians (see reference 18 for details). The Japanese-American urine donors were patients at the Nikkei Medical Center in the Little Tokyo neighborhood of Los Angeles. Their parents and grandparents were all Japanese. The urine samples from other southern Californians were collected from the general patient population at the Scripps Clinic, La Jolla, CA. This study was approved by the Human Subjects Committee o...