Age-specific alterations in muscarinic stimulation of K+-evoked dopamine release from striatal slices by cholesterol andS-adenosyl-l-methionine

Joseph, James A.; Villalobos‐Molina, Rafael; Yamagami, Keiji; Roth, George S.; Kelly, Jeremiah F.

doi:10.1016/0006-8993(94)01311-5

Cited by 22 publications

(8 citation statements)

References 44 publications

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“…A recent study found that incubation of striatal slices of 6-month-old rats with cholesterol produced a similar effect on potassium-evoked dopamine release as observed in 24-month-old rats (Joseph et al, 1995). Addition of cholesterol to membranes would certainly alter the cholesterol distribution of the exofacial and cytofacial leaflets and change the cholesterol-to-phospholipid molar ratio of each individual leaflet.…”

Section: Discussionmentioning

confidence: 86%

Increasing Age Alters Transbilayer Fluidity and Cholesterol Asymmetry in Synaptic Plasma Membranes of Mice

Igbavboa

Avdulov

Schroeder

et al. 1996

Journal of Neurochemistry

154

110

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Previous studies examining age differences in membrane fluidity and cholesterol content have reported on the average or total change in membrane structure, respectively. However, a membrane consists of an exofacial leaflet and a cytofacial leaflet that differ in fluidity and cholesterol distribution. The purpose of the present experiments was to determine fluidity and cholesterol distribution of the exofacial and cytofacial leaflets of brain synaptic plasma membranes (SPMs) from 3-4-, 14-15-, and 24-25-month-old C57BL/6NNIA mice by using trinitrobenzenesulfonic acid (TNBS)-quenching techniques and fluorescent probes. The exofacial leaflet of SPMs from young mice was significantly more fluid compared with the cytofacial leaflet. The large difference in fluidity between the two leaflets was abolished in SPMs of the oldest age group. Total SPM cholesterol and the cholesterol-to-phospholipid molar ratio did not differ among the three different age groups of mice. However, considerable differences were observed in the distribution of cholesterol in the two SPM leaflets. The exofacial leaflet contained substantially less cholesterol than did the cytofacial leaflet (13 vs. 87%, respectively) in SPMs of young mice. This asymmetric distribution of cholesterol was significantly modified with increasing age. There was an approximately twofold increase in exofacial leaflet cholesterol in the oldest group compared with the youngest age group. Transbilayer fluidity and cholesterol asymmetry were altered in SPMs of older mice. This approach is a new and different way of viewing how aging modifies membrane structure. Age differences in SPM leaflet structure may be an important factor regulating activity of certain membrane proteins. Key Words: Fluidity-Cholesterol-Brain-Synaptic plasma membrane-Lipid asymmetry-Aging-Mice.

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Section: Discussionmentioning

confidence: 86%

Increasing Age Alters Transbilayer Fluidity and Cholesterol Asymmetry in Synaptic Plasma Membranes of Mice

Igbavboa

Avdulov

Schroeder

et al. 1996

Journal of Neurochemistry

154

110

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“…There may also be properties of the phytochemicals contained in spinach, strawberries and blueberries that may produce eects other than antioxidant protection. There is evidence that¯avonoid compounds can increase membrane¯uidity [44,119,144], and a previous experiment has shown [59] that experimental decreases in membrane rigidity (via s-adenosyll-methionine) can ameliorate de®cits in striatal signal transduction in old animals. Phytonutrients (e.g., anthocyanins and other¯avonoids) contained in the fruits and vegetable have also been shown to antagonize arachidonic acid transport [64] and suppress the 5lipoxygenase pathway [86] and thus reduce in¯ammatory responses [11,36].…”

Section: Fruits and Vegetablesmentioning

confidence: 99%

Neurobehavioral aspects of antioxidants in aging

Cantuti-Castelvetri¹,

Shukitt‐Hale²,

Joseph³

2000

Intl J of Devlp Neuroscience

205

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Both aging and age-associated neurodegenerative diseases are associated with various degrees of behavioral impairments, and among the prime candidates responsible for producing the neuronal changes mediating these behavioral deficits appear to be free radicals and the oxidative stress they generate. Therefore, there have been a number of studies which have examined the putative positive benefits of antioxidants in altering, reversing, or forestalling these neuronal/behavioral decrements, with varying degrees of success. Additional experiments have examined the effects of diets rich in fruits and vegetables or herbal extracts in reducing certain types of cancer and cardiovascular diseases, and evidence emerging from such experiments suggests that these kinds of dietary modifications may be beneficial in altering neuronal/behavioral deficits in aging, as well. These kinds of diets are particularly rich in antioxidants such as vitamins A, C, E, and bioflavonoids (such as flavones, tannins, and anthocyanins), and thus, there may be synergistic effects among them. The present paper will review studies concerning the influence of dietary and synthetic antioxidants on normal, pathological age-related, and reactive oxygen species-induced behavioral changes in human and animal subjects. The antioxidants reviewed are vitamin E, alpha-lipoic acid, and the phytochemicals contained in herbals, fruits and vegetables.

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“…Loss of MAChRs has also been observed in neurodegenerative AD [154,155] and we have shown that it is exacerbated by oxidative stress [156]. There is also strong evidence linking MAChRs to various aspects of amyloid precursor protein processing and vascular functioning [157,158].…”

Section: Muscarinic Receptors In Cos-7 Cellsmentioning

confidence: 62%