Age Stratification in Genetic Variation of Lipoprotein Lipase in Metabolic Syndrome Javanese Ethnics of Indonesia

Abstract

BACKGROUND: Metabolic syndrome (Met-S) that caused by heredity and Lipoprotein Lipase (LPL). LPL is involved in the metabolism of serum lipids. Variations in LPL alter enzyme activity, and the most common variations are LPL +495 T > G and LPL Pvu II C > T.
 AIM: This study aimed to identify the role of LPL +495 T > G and LPL PvuII C > T gene variations in subjects with Met-S in Javanese ethnic based on age stratification.
 METHODS: We recruited 160 participants of Javanese ethnicity consisting of 80 cases and 80 control subjects. Met-S was diagnosed according to the criteria of NCEP ATP III. Peripheral blood samples were collected to determine biochemical parameters. Screening for both polymorphisms was made by PCR-RFLP.
 RESULTS: Results found that genotype and allele frequencies for LPL +495 T > G were not significantly different between Met-S and controls with and without age stratification. In LPL PvuII C > T based on age stratification, there were significant differences between TT vs CC, recessive and dominant models in Met-S and control. In the age group > 45 years CC genotypes and TC+CC had increased risk of Met-S compared to TT genotypes. In summary, there was no significant association between LPL +495 T > G gene variation with Met-S.
 CONCLUSION: In LPL PvuII gene variation, TC + CC is the risk genotype of Met-S in the age group > 45 years.

Risk factor of metabolic syndrome in Javanese population based on determinants of anthropometry and metabolic measurement

“…As for the Met-S criteria as a combination of three or more components based on the following components: increased waist circumference (WC), increased triglycerides (TG), reduced high-density lipoproteins -cholesterol (HDL-C-Chol), increased blood pressure (BP), and increase fasting plasma glucose (FPG). 10 Waist to height ratio (WHtR) was obtained based on the measurement of WC against height; central obesity based on WC > 80 cm in female and WC > 90 cm in male; and total cholesterol/ HDL-C ratio was obtained based on total cholesterol to HDL-C and categorized into two categories <5 and >5.…”