Age validation of the growth lamellae in the cuttlebone from cultured Sepia pharaonis at different stages

Determination of squid age using upper beak rostrum sections: technique improvement and comparison with the statolith

“…In addition, D. gigas from warm waters (Ecuador) have wider increments than those from cold waters (Chile) ( Table 2), as found in beaks of O. vulgaris LWS (Canali et al 2011). Such a pattern is also congruent with the findings in statolith (Villanueva 2000) and in cuttlebone (Chung and Wang 2013).…”

“…In recent years, laboratory culture and marking have been the most popular validation methods for examining the periodicity of increment formation in sepioid cuttlebone (Bettencourt and Guerra 2001;Chung and Wang 2013) and octopod stylet (Doubleday et al 2006;Hermosilla et al 2010), lenses (Rodríguez-Domínguez et al 2013) and beaks (Oosthuizen 2003;Bárcenas et al 2014). By comparing the increment number with the actual age and elapsed days after marking or between two marks, daily deposition of the growth increments in beaks was validated for two octopods, Octopus vulgaris (Oosthuizen 2003;Canali et al 2011;Perales-Raya et al 2014a, b) and Octopus maya (Rodríguez-Domínguez et al 2013;Bárcenas et al 2014).…”

Elemental Ratios in Cuttlebone Indicate Growth Rates in the Cuttlefish Sepia pharaonis

“…The components of lamellae are based on an organic framework filled with calcium carbonate in aragonite form (Florek et al, 2009). Previous studies on cuttlebone structure have focused on the number of lamellae, each of which is formed over approximately 1.75 days; thus, they offer a mechanism for estimating the age of cuttlefish (Bettencourt and Guerra, 2001;Chung and Wang, 2013). Cuttlebone growth during ontogenetic development is through continuous lamellar formation.…”

“…Cuttlebone growth during ontogenetic development is through continuous lamellar formation. Although one layer does not follow the 1-day rule (Bettencourt and Guerra, 2001;Chung and Wang, 2013), the cuttlebone still records environmental and internal physiological signals over its lifetime.…”

Cuttlebone morphometrics and sex identification of Sepia bertheloti (d’Orbigny, 1835) from the central-east Atlantic

“…The cuttlebone shows a structure finely laminated (lamellas). According to Bettencourt and Guerra [9] and Chung and Wang [10], the periodicity of lamellar deposition depends on a combination of physiological and environmental factors (i.e., water temperature).…”