Aged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia model

Orozco-Ibarra, Marisol; Muñoz‐Sánchez, Jorge; Zavala-Medina, Martín E.; Pineda, Benjamín; Magaña-Maldonado, Roxana; Vázquez‐Contreras, Edgar; Maldonado, Perla D.; Pedraza‐Chaverrí, José; Chánez‐Cárdenas, María Elena

doi:10.1186/s40659-016-0067-6

Cited by 25 publications

(19 citation statements)

References 40 publications

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“…19 More recently, Orozco-Ibarra and his colleagues reported that AGE and its main constituent S-allylcysteine (SAC) are natural antioxidants that have protective effects against cerebral ischemia. 20 As aforementioned, the enhancing CNS excitability effect of PTZ may be owed to reduction of GABA-ergic inhibition, the present work illustrated how administrations of AGE prior to induction of convulsions by PTZ; alter the cellular brain levels of the major inhibitory neurotransmitter GABA. Results have shown significant increase in GABA levels (P < 0.05) in rats given AGE 5 minutes prior to 60 mg/kg PTZ compared with control group and PTZtreated group.…”

Section: Effect Of Age Pretreatment On Cd50 Of Ptzsupporting

confidence: 55%

Protective Effect of Garlic Extract Against Pentylenetetrazole Induced Seizures

Thabet¹,

Alshaibany²,

Alenezi³

et al. 2016

IJMRP

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Background and Aims: Previous studies in literature reported that compared with fresh preparations of garlic and aged garlic extract (AGE) contain high amount of antioxidants and protect against cancer, aging and many cardiovascular diseases. The present work investigates effect of AGE against pentylenetetrazole (PTZ)-induced convulsions and brain levels of Gamma amino butyric acid (GABA). Methods: The least and median convulsive (CD50) doses of PTZ were determined either alone or after pretreatment with AGE. The forebrain levels of the neurotransmitter GABA have been measured in rats given PTZ, alone and after pretreatment with AGE. Results: The present study showed that AGE pretreatment elicited a dose dependent protection of rats against PTZinduced seizures. AGE in a dose of 400 mg 60 minutes prior to 60mg/kg PTZ produced 80% decrease in incidence of seizures. AGE pretreatment produced significant elevation of the median convulsive dose (CD50) of PTZ from 47 mg/kg to 55 mg/kg. Results have shown that cellular brain GABA concentrations decreased significantly (P < 0.05) in rats given 400 mg/kg AGE 60 minutes prior to 60 mg/kg PTZ compared with control and PTZ-treated groups. Conclusion: The present work suggests a potential neuroprotective effect of AGE against PTZ-induced seizures that may be explained by increasing brain levels of the major central inhibitory neurotransmitter GABA.

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Section: Effect Of Age Pretreatment On Cd50 Of Ptzsupporting

confidence: 55%

Protective Effect of Garlic Extract Against Pentylenetetrazole Induced Seizures

Thabet¹,

Alshaibany²,

Alenezi³

et al. 2016

IJMRP

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“…However, further increase in CoCl 2 concentration triggers apoptotic cell death and population of cells in G0/G1 phase increases significantly in both cells which may due to increase in extent of hypoxia by further increase in CoCl 2 concentration that drags cells towards apoptosis [26]. Morphological study also confirms the cell proliferation that occurs in a dose dependent manner up to certain concentration in both the cell lines (MCF-7, 50–150 µM; MDA-MB-231, 10–25 µM) and further increase in CoCl 2 concentration disturbs the cellular morphology which ultimately leads to cell death [27]. It was reported that CoCl 2 artificially induce the hypoxia by blocking the degradation of HIF-1α [18, 19] and when HIF-1α gets activated it further stimulates transcription of hypoxia associated genes like VEGF which promotes angiogenesis [20].…”

Section: Discussionmentioning

confidence: 77%

CoCl2 simulated hypoxia induce cell proliferation and alter the expression pattern of hypoxia associated genes involved in angiogenesis and apoptosis

2019

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Background/aimsHypoxia microenvironment plays a crucial role during tumor progression and it tends to exhibit poor prognosis and make resistant to various conventional therapies. HIF-1α acts as an important transcriptional regulator directly or indirectly associated with genes involved in cell proliferation, angiogenesis, apoptosis and energy metabolism during tumor progression in hypoxic microenvironment. This study was aimed to investigate the expression pattern of the hypoxia associated genes and their association during breast cancer progression under hypoxic microenvironment in breast cancer cells.MethodsCell proliferation in MCF-7 and MDA-MB-231 cell lines treated with different concentration of CoCl2 was analyzed by MTT assay. Flow cytometry was performed to check cell cycle distribution, whereas cell morphology was examined by phase contrast microscopy in both the cells during hypoxia induction. Expression of hypoxia associated genes HIF-1α, VEGF, p53 and BAX were determined by semiquantitative RT-PCR and real-time PCR. Western blotting was performed to detect the expression at protein level.ResultsOur study revealed that cell proliferation in CoCl2 treated breast cancer cells were concentration dependent and varies with different cell types, further increase in CoCl2 concentration leads to apoptotic cell death. Further, accumulation of p53 protein in response to hypoxia as compare to normoxia showed that induction of p53 in breast cancer cells is HIF-1α dependent. HIF-1α dependent BAX expression during hypoxia revealed that after certain extent of hypoxia induction, over expression of BAX conquers the effect of anti-apoptotic proteins and ultimately leads to apoptosis in breast cancer cells.ConclusionIn conclusion our results clearly indicate that CoCl2 simulated hypoxia induce the accumulation of HIF-1α protein and alter the expression of hypoxia associated genes involved in angiogenesis and apoptosis.Electronic supplementary materialThe online version of this article (10.1186/s40659-019-0221-z) contains supplementary material, which is available to authorized users.

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“…Aged garlic is a processed garlic product that has been ripened under controlled temperature and humidity conditions for more than 1 month, and it contains a greater amount of SAC than fresh garlic [14]. Aged garlic has been reported to protect the small intestine tissue from chemical-induced damage [15] and prevent apoptotic cell death [16], which is believed to be a result of SAC activity.…”

Section: Introductionmentioning

confidence: 99%

Oral Administration of (S)-Allyl-l-Cysteine and Aged Garlic Extract to Rats: Determination of Metabolites and Their Pharmacokinetics

Park

Lee

et al. 2017

Planta Med

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()-Allyl-l-cysteine is the major bioactive compound in garlic. ()-Allyl-l-cysteine is metabolized to ()-allyl-l-cysteine sulfoxide, -acetyl-()-allyl-l-cysteine, and -acetyl-()-allyl-l-cysteine sulfoxide after oral administration. An accurate LC-MS/MS method was developed and validated for the simultaneous quantification of ()-allyl-l-cysteine and its metabolites in rat plasma, and the feasibility of using it in pharmacokinetic studies was tested. The analytes were quantified by multiple reaction monitoring using an atmospheric pressure ionization mass spectrometer. Because significant quantitative interference was observed between ()-allyl-l-cysteine and -acetyl-()-allyl-l-cysteine as a result of the decomposition of -acetyl-()-allyl-l-cysteine at the detector source, chromatographic separation was required to discriminate ()-allyl-l-cysteine and its metabolites on a reversed-phase C analytical column with a gradient mobile phase consisting of 0.1% formic acid and acetonitrile. The calibration curves of ()-allyl-l-cysteine, ()-allyl-l-cysteine sulfoxide, -acetyl-()-allyl-l-cysteine, and -acetyl-()-allyl-l-cysteine sulfoxide were linear over each concentration range, and the lower limits of quantification were 0.1 µg/mL [()-allyl-l-cysteine and -acetyl-()-allyl-l-cysteine] and 0.25 µg/mL [()-allyl-l-cysteine sulfoxide and -acetyl-()-allyl-l-cysteine sulfoxide]. Acceptable intraday and inter-day precisions and accuracies were obtained at three concentration levels. The method satisfied the regulatory requirements for matrix effects, recovery, and stability. The validated LC-MS/MS method was successfully used to determine the concentration of ()-allyl-l-cysteine and its metabolites in rat plasma samples after the administration of ()-allyl-l-cysteine or aged garlic extract.

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Aged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia model

Cited by 25 publications

References 40 publications

Protective Effect of Garlic Extract Against Pentylenetetrazole Induced Seizures

Protective Effect of Garlic Extract Against Pentylenetetrazole Induced Seizures

CoCl2 simulated hypoxia induce cell proliferation and alter the expression pattern of hypoxia associated genes involved in angiogenesis and apoptosis

Oral Administration of (S)-Allyl-l-Cysteine and Aged Garlic Extract to Rats: Determination of Metabolites and Their Pharmacokinetics

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