Aged garlic extract attenuates gentamicin induced renal damage and oxidative stress in rats

Maldonado, Perla D.; Barrera, Diana; Medina-Campos, Omar Noel; Hernández-Pando, Rogelio; Ibarra-Rubio, María Elena; Pedraza‐Chaverrí, José

doi:10.1016/s0024-3205(03)00609-x

Cited by 127 publications

(104 citation statements)

References 40 publications

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“…These results could be in accord with several other researches, which reported that, compounds with antioxidant properties like S-allylmercaptocysteine 40) and diallyl sulfide 41) inhibited the increased urinary excretion of total protein induced by GM in rats. Other compounds like resveratrol, 22) carnosine 23) or garlic extract, 8) partially prevented the increase in BUN and serum creatinine levels induced by GM. In the present study, the role of ROS in GM-induced nephrotoxicity was assessed by the usage of antioxidant agent, quercetin, and further evaluation of alterations in the biochemical indicators of oxidative stress mainly GSH, TBARS levels, SOD and CAT activities beside histological changes.…”

Section: Discussionmentioning

confidence: 96%

“…However, GM has been shown to enhance the generation of reactive oxygen species (ROS) 6,7) causing deficiency in intrinsic antioxidant enzymes. 8) ROS have been suggested as a cause of death for many cells in different pathological states including various models of renal and cardiac diseases. [9][10][11] Accordingly, the use of several compounds with antioxidant activity has been successfully used to prevent or ameliorate GM-induced nephrotoxicity.…”

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confidence: 99%

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Protective Effect of Quercetin against Gentamicin-Induced Nephrotoxicity in Rats

Abdel-Raheem

Abdelghany

Mohamed

2009

Biological & Pharmaceutical Bulletin

156

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Gentamicin (GM) is an antibiotic widely used in treating severe gram-negative infections. However, its clinical use is limited by its nephrotoxicity. Several lines of evidence indicate that free radicals are important mediators of gentamicin nephrotoxicity. Therefore, the aim of this work was to investigate the possible protective effect of the flavonoid quercetin, an antioxidant, on gentamicin-induced nephrotoxicity. For this purpose, rats were divided into four groups. First group served as a control and injected with the normal saline, second group was injected with quercetin (50 mg/kg/d, per os) for 7 d, third group was injected with gentamicin (80 mg/kg/d, intraperitoneally) for 7 d and the fourth group of animals was injected with quercetin plus gentamicin simultaneously for 7 d. Total protein levels were estimated in 24-h urine samples to assess kidney dysfunction. The rats were sacrificed on the seventh day and kidneys were collected for histopathological studies. Blood urea nitrogen (BUN) and creatinine levels were measured in the blood. Moreover, glutathione (GSH), lipid peroxide (TBARS) levels, superoxide dismutase (SOD) and catalase (CAT) activities were determined in renal tissues. GM-treated rats showed early kidney dysfunction as urinary total protein, BUN and serum creatinine levels were significantly increased. The significant decrease in GSH levels, SOD, CAT activities and increase in TBARS levels, indicated that GMinduced nephrotoxicity was mediated through oxidative stress reactions. Histopathological examination of GM-treated rats revealed degenerative changes in glomeruli and tubules. On the other hand, simultaneous administration of quercetin plus gentamicin protected kidney tissues against nephrotoxic effects of gentamicin as evidenced from amelioration of histopathological changes and normalization of kidney biochemical parameters.

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Section: Discussionmentioning

confidence: 96%

mentioning

confidence: 99%

Protective Effect of Quercetin against Gentamicin-Induced Nephrotoxicity in Rats

Abdel-Raheem

Abdelghany

Mohamed

2009

Biological & Pharmaceutical Bulletin

156

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“…A slight increase in GM blood concentration is accompanied by a significant, incommensurate increase in the amount of the drug-bound renal cortex. [16,34,35] Several investigators [36,37] reported that treatments with GM produces nephrotoxicity, as is evident by the reduction in renal function, which is characterized by an increase in serum creatinine and BUN level accompanied by impairment in glomerular functions. Serum creatinine level is more significant than the BUN levels in the earlier phase of the renal damage.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Pentoxifylline Treatment on Gentamicin-Induced Nephrotoxicity in Rats

et al. 2009

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Gentamicin (GM) is a widely used antibiotic against serious and life-threatening infections, but its usefulness is limited by the development of nephrotoxicity. Thus, the present study was undertaken to determine if pentoxifylline could protect the kidney in this experimental model. Thirty male Wistar rats were used. The animals were divided into three groups, each with 10 animals. The GM group of animals was treated daily with gentamicin in a dose of 100 mg/kg for eight days. The GMP group of animals was treated daily with pentoxifylline in a dose of 45 mg/kg and the same dose of gentamicin as the GM group for eight days. The control group received 1 mL/day saline intraperitoneally. For histological analysis, 5 μm-thick sections were stained with hematoxylin and eosin (HE), periodic acid Schiff (PAS), and Jones methenamine silver. The morphometric parameters included were glomerular area, major and minor axis, perimeter, diameter, roundness, and mean optical density. Biochemical analyses were used to determine concentrations of blood urea, serum creatinine, sodium, and potassium. In the GM group of rats, glomerular basement membrane was diffusely and unequally thickened with polymorphonuclear leukocyte infiltration, and coagulation-type necrosis and vacuolization of cytoplasm of proximal tubules epithelial cells were observed. In the GMP group of rats, glomeruli were slightly enlarged with thickened basement membrane in some segments but without coagulation-type necrosis of proximal tubules epithelial cells. Blood urea and serum creatinine concentration in the GM group were significantly elevated in comparison with the GMP group, while the potassium level was decreased. The present study indicated that pentoxifylline could provide a marked protective effect against gentamicin-induced acute renal failure, most likely mediated by vascular decongestion.

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“…Pedraza-Chaverri et al have described that the renal damage caused by gentamicin could be reduced with a garlic diet (7). Maldonado et al have fed aged garlic extracts to rats administered with gentamicin, and determined that these extracts' antioxidant effects prevented the increase of carbonyl and nitrotyrosine levels in the renal cortex (8).…”

Section: Introductionmentioning

confidence: 99%

Garlic supplemented diet attenuates gentamicin nephrotoxicity ın rats

et al. 2014

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ARTICLE INFO ______________________________________________________________ ______________________Purpose: To demonstrate the effect of a 4% pulverized garlic supplemented diet on the nephrotoxicity induced by gentamicin in rats. Materials and Methods: Twenty four healthy male Wistar rats, weighing between 220 -260grams, were divided into three groups. The rats were randomly assigned to either the gentamicin injection without garlic supplementation group (Group I, n = 8), gentamicin injection with garlic supplementation group (Group II, n = 8), and control group (Group III, n = 8). Urine from the rats was collected and the volume (mLand pH values were measured. Then urea (mg/dL), creatinine (mg/dL), total protein (g/dL) and cystatin (mg/L) values were measured for the blood samples obtained from tail veins. Results:The median NAG value for the control group (52.050 U/L) was similar to value for Group II (56.400 U/L), which received gentamicin and the garlic diet. However, the median NAG value for Group I (77.030 U/L), which received gentamicin without garlic supplementation, was determined to be statistically significantly higher (p = 0.010) than the value for the control group. In addition, the mean cystatin value for Group II (1.360 U/L) was found to be statistically significantly lower than the value for the Group I (2.240 U/L) (p = 0.015).Conclusions: In this study we showed the effect of 4% pulverized garlic supplemented diet for preventing nephrotoxicity induced by gentamicin in rats by using as parameters NAG in urine samples and cystatin C in serum samples.

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Aged garlic extract attenuates gentamicin induced renal damage and oxidative stress in rats

Cited by 127 publications

References 40 publications

Protective Effect of Quercetin against Gentamicin-Induced Nephrotoxicity in Rats

Protective Effect of Quercetin against Gentamicin-Induced Nephrotoxicity in Rats

Protective Effects of Pentoxifylline Treatment on Gentamicin-Induced Nephrotoxicity in Rats

Garlic supplemented diet attenuates gentamicin nephrotoxicity ın rats

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